Barley Cultivar Identification by SDS Gradient Page Analysis of Hordein

Marchylo, B. A.

doi:10.4141/cjps87-131

Cited by 20 publications

(16 citation statements)

References 8 publications

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“…Samples were of three hulless barley cultivars registered in western Canada, CDC Buck, Condor and Falcon. The cultivars were confirmed using SDS PAGE analysis of hordeins (Marchylo 1987).…”

Section: Methodsmentioning

confidence: 99%

The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley

Clear¹,

Patrick²,

Nowicki³

et al. 1997

Can. J. Plant Sci.

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. 1997. The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley. Can. J. Plant Sci. 77: 161-166. Fourteen samples consisting of three cultivars of hulless barley (Hordeum vulgare L.), grown commercially in southern Manitoba in 1994, were examined for levels of Fusarium species and seven fusarium trichothecene mycotoxins: deoxynivalenol (DON), diacetoxyscirpenol, 15-acetyldeoxynivalenol (15ADON), fusarenone-X, HT-2 toxin, neosolaniol and T-2 toxin. Four fractions from each sample were analysed. These consisted of kernels with the hulls still attached, kernels whose hulls had been removed at harvest, kernels that had been dehulled in the laboratory using a deawner, and kernels that had been pearled in the laboratory to 60% of their original weight. A composite sample of the hulls, obtained from the laboratory dehulling, was also tested. Mycotoxins were determined by gas chromatography-mass spectrometry. DON and 15ADON were the only trichothecenes detected in this study. The greatest numbers of propagules of Fusarium spp. and the highest concentrations of DON and 15ADON were found in the hulls. The average concentrations of F. graminearum Schwabe and DON in barley were reduced: 90 and 49%, respectively, as a consequence of laboratory dehulling; 95 and 59%, respectively, as a result of dehulling during harvest; and 99 and 86%, respectively, as a result of pearling. Results suggest that growing hulless cultivars shows promise for management of mycotoxin problems associated with FHB.

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“…Samples were of three hulless barley cultivars registered in western Canada, CDC Buck, Condor and Falcon. The cultivars were confirmed using SDS PAGE analysis of hordeins (Marchylo 1987).…”

Section: Methodsmentioning

confidence: 99%

The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley

Clear¹,

Patrick²,

Nowicki³

et al. 1997

Can. J. Plant Sci.

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“…Characteristics of importance for malting quality, which can differ considerably among barley cultivars, include grain size, grain protein concentration, amount of extract obtained from the malt, diastatic power and seed nitrogen (Morgan et al, 1981;Henry, 1990;Weston et al, 1993;Eagles et al, 1995). phoresis is particularly attractive as a screening test to differentiate cultivars (Shewry et al, 1980a;Marchylo, 1987) and to determine malting quality (Baxter & Wainwright, 1979;Shewry et al, 1980a). Hordein has also been investigated through different approaches including RFLP analysis (Shewry et al, 1985;Molnar & McKay, 1991), DNA sequencing (Brandt et al, 1985;Entwistle, 1988;Chernyshev et al, 1989;Vicente-Carbajosa et al, 1992;Kanazin et al, 1993), analysis of the regulatory control of hordein genes , and determination of the mRNA encoded by each locus (Kreis et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties

Echart-Almeida

Cavalli-Molina

2001

Pesq. agropec. bras.

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-Since there is evidence that malting quality is related to the storage protein (hordein) fraction, in the present work the hordein polypeptide patterns from 13 barley (Hordeum vulgare L.) varieties of different malting quality were analysed in order to explore the feasibility of using hordein electrophoresis to assist in the selection of malting barleys. The formation of clusters separating the varieties with higher malting quality from the others with lower quality suggests that there is a relationship between the general hordein polypeptide pattern and malting quality in the varieties analysed. By the Sperman's correlation test three hordein bands correlated negatively with malting quality in the germplasm studied.Index terms: Hordeum vulgare, storage proteins, electrophoresis. Padrões de polipeptídios de hordeína em relação à qualidade de malteação em variedades brasileiras de cevadaResumo -No presente trabalho foi analisado o padrão de polipeptídios de hordeína de 13 variedades de cevada (Hordeum vulgare L.) que apresentam diferentes qualidades de malteação, com o objetivo de verificar a viabilidade de se usar eletroforese de hordeínas na seleção de cevadas usadas para malteação. A formação de agrupamentos separando as variedades que apresentam altos níveis de malteação daquelas com baixa qualidade sugere haver uma clara associação entre a qualidade de malteação e os padrões de hordeína, pelo menos com relação às variedades analisadas. Pelo teste de correlação de Sperman, três bandas apresentaram correlação negativa com a qualidade de malteação.Termos para indexação: Hordeum vulgare, proteínas de reserva, eletroforese.

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“…The resultant HMW and LMW glutenin subunits are soluble in 50% l-propanol (Shewry et al 1986). Although it has been demonstrated that the addition of l% acetic acid slightly increases protein extraction (Byers et al 1983 (Marchylo 1987). The LMW standard proteins were solubilized, reduced and alkylated essentially as before (Marchylo 1987) (LKB, Bromma, Sweden) with cell dimensions of 16 x 18 x 0.075 cm.…”

mentioning

confidence: 99%

“…Although it has been demonstrated that the addition of l% acetic acid slightly increases protein extraction (Byers et al 1983 (Marchylo 1987). The LMW standard proteins were solubilized, reduced and alkylated essentially as before (Marchylo 1987) (LKB, Bromma, Sweden) with cell dimensions of 16 x 18 x 0.075 cm. Low-density (LD), highdensity (HD) and stacking gel solutions (6% T + 2% Q were prepared according to the recipes described previously (Marchylo 1987) with the exception that 6.50 mL of 50% acrylamide and 12.7 5 mL of water were used in preparation of the low density solution.…”

mentioning

confidence: 99%

“…The LMW standard proteins were solubilized, reduced and alkylated essentially as before (Marchylo 1987) (LKB, Bromma, Sweden) with cell dimensions of 16 x 18 x 0.075 cm. Low-density (LD), highdensity (HD) and stacking gel solutions (6% T + 2% Q were prepared according to the recipes described previously (Marchylo 1987) with the exception that 6.50 mL of 50% acrylamide and 12.7 5 mL of water were used in preparation of the low density solution. Linear gradient gels and wells (2olgel) were prepared; samples were injected (lpllwell); electrophoresis; subsequent staining with a silver-based color stain and densitometry were carried out as previously described (Marchylo 1987) with the exception that electrophoresis was performed over 2 h. Migration distances of protein bands were determined by ruled measurement.…”

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confidence: 99%

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Barley Cultivar Identification by SDS Gradient Page Analysis of Hordein

Cited by 20 publications

References 8 publications

The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley

The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley

Hordein polypeptide patterns in relation to malting quality in Brazilian barley varieties

Identification of Canadian and American Wheat Cultivars by SDS Gradient Page Analysis of Gliadin and Glutenin Subunits

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