Barley Proteomics

Mock, Hans‐Peter; Finnie, Christine; Witzel, Katja; Svensson, Birte

doi:10.1007/978-3-319-92528-8_19

Cited by 3 publications

(6 citation statements)

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“…proteomics and in situ microscopic analyses enable the mapping of the endomembrane system of developing barley endosperm. Many proteomic analyses in barley grain allow us to identify and understand the molecular composition of developing barley grain [55][56][57] . However, less is known about proteins regulating the endomembrane system in developing barley grains.…”

Section: Discussionmentioning

confidence: 99%

Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s

Roustan

Hilscher

Weidinger

et al. 2020

Sci Rep

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cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (pBs) and protein storage vacuoles (pSVs), for the accumulation of storage proteins. During development, protein trafficking and storage require an extensive reorganization of the endomembrane system. Consequently, endomembrane-modifying proteins will influence the final grain quality and yield. However, little is known about the molecular mechanism underlying endomembrane system remodeling during barley grain development. By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. Clustering analysis allowed characterization of three different development phases; notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. Moreover, endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. Specifically, multivesicular bodies (MVBs), and the ESCRT-III HvSNF7 proteins are associated with PBs during barley endosperm development. Together our data identified promising targets to be genetically engineered to modulate seed storage protein accumulation that have a growing role in health and nutritional issues. After differentiation, fully developed cereal endosperm makes up to 75% of the grain weight and covers four major cell types: aleurone, starchy endosperm, transfer cells, and the cells of the embryo surrounding region 1. The starchy endosperm thereby is characterized as a storage site, accumulating starch and seed storage proteins (SSPs) 2. The aleurone layer plays essential roles during seed germination and mobilizes starch and SSP reserves in the starchy endosperm by releasing hydrolytic enzymes that are responsible for the degradation of stored nutrients in the endosperm 2. Contrary to the persistent endosperm of cereals, the cellular endosperm of Arabidopsis thaliana (A. thaliana) supports the developing and growing embryo, resulting in a gradually depleted endosperm as the embryo grows. Finally, the massive A. thaliana embryo is only accompanied by a single peripheral layer, the aleurone layer, in mature seeds 2. Consequently, A. thaliana cannot to be used as a model system to study the endomembrane system in grain endosperm.

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Section: Discussionmentioning

confidence: 99%

Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s

Roustan

Hilscher

Weidinger

et al. 2020

Sci Rep

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“…Many proteomic analyses in barley grain allow us to identify and understand the molecular composition of developing barley grain (Finnie et al, 2002;Finnie et al, 2011;Mock et al, 2018). However, less is known about proteins regulating the endomembrane system in developing barley grains.…”

Section: Proteomics and In Situ Microscopic Analyses Enable The Mappimentioning

confidence: 99%

Cytoskeleton members, MVBs and the ESCRT-III HvSNF7s are putative key players for protein sorting into protein bodies during barley endosperm development

Roustan

Hilscher

Weidinger

et al. 2019

Preprint

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Twitter: @VerenaIbl m: 4; f: 7 Abbreviations AMSH1, associated molecule with the SH3 domain of Stam1; A. AbstractCereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and protein storage vacuoles (PSVs), for the accumulation of storage proteins. PBs can be used as efficient biotechnological systems to produce high yields of stable recombinant proteins. During development, protein trafficking and storage require an extensive reorganization of the endomembrane system. Consequently, endomembrane-modifying proteins will influence the final grain quality, yield and recombinant protein production. Barley, a cereal crop of worldwide importance for the brewing industry, animal feed and to a lesser extent, human nutrition, has been identified as promising candidate for recombinant protein production. However, little is known about the molecular mechanism underlying endomembrane system remodeling during barley grain development. By using in vivo label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. Based on proteome annotation and a homology search, 95 proteins associated with the endomembrane system were identified, and 83 of these exhibited significant changes in abundance during grain development. Clustering analysis allowed characterization of three different development stages; notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified protein bodies at the early development stages. Endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. Specifically, multivesicular bodies (MVBs), and the ESCRT-III HvSNF7 proteins are associated with protein bodies (PBs) during barley endosperm development. Taken together, our proteomics results specifically identified members of the cytoskeleton, MVBs, and ESCRT as putative key players for protein sorting into PBs during barley endosperm development. These results present a comprehensive overview of proteins involved in the rearrangement of the endomembrane system during barley early grain development and will provide the basis for future work on engineering the endomembrane system to optimize nutrient content and to produce high yields of recombinant proteins.

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“…Functional analysis of proteins is often achieved by qualitative and quantitative measurements of plant tissues at specific developmental or physiological stages. 24 − 26 …”

Section: Introductionmentioning

confidence: 99%

“…The results obtained from proteomics can be beneficial in many different research fields, for instance, to provide information about detection of diagnostic markers and alteration of expression patterns in response to different signals to understand pathogenicity mechanisms. − In agriculture and food studies, proteomics is applied for the identification and characterization of proteins and to elucidate their function and interactions. Functional analysis of proteins is often achieved by qualitative and quantitative measurements of plant tissues at specific developmental or physiological stages. − …”

Section: Introductionmentioning

confidence: 99%

“…There have been several valuable reviews covering different aspects of crops, particularly in barley which the reader can refer to for further information. − In recent years, advances in MS-based tools and methods have resulted in increasing application of proteomics to barley employing a range of different techniques. This review aims to give an overview of barley proteomics studies and to highlight the application of mass spectrometry to different parts of the value chain from cultivation to processing and final product quality and safety.…”

Section: Introductionmentioning

confidence: 99%

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Application of Mass Spectrometry-Based Proteomics to Barley Research

Bahmani

O'Lone

Juhász

et al. 2021

J. Agric. Food Chem.

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Barley ( Hordeum vulgare ) is the fourth most cultivated crop in the world in terms of production volume, and it is also the most important raw material of the malting and brewing industries. Barley belongs to the grass (Poaceae) family and plays an important role in food security and food safety for both humans and livestock. With the global population set to reach 9.7 billion by 2050, but with less available and/or suitable land for agriculture, the use of biotechnology tools in breeding programs are of considerable importance in the quest to meet the growing food gap. Proteomics as a member of the “omics” technologies has become popular for the investigation of proteins in cereal crops and particularly barley and its related products such as malt and beer. This technology has been applied to study how proteins in barley respond to adverse environmental conditions including abiotic and/or biotic stresses, how they are impacted during food processing including malting and brewing, and the presence of proteins implicated in celiac disease. Moreover, proteomics can be used in the future to inform breeding programs that aim to enhance the nutritional value and broaden the application of this crop in new food and beverage products. Mass spectrometry analysis is a valuable tool that, along with genomics and transcriptomics, can inform plant breeding strategies that aim to produce superior barley varieties. In this review, recent studies employing both qualitative and quantitative mass spectrometry approaches are explored with a focus on their application in cultivation, manufacturing, processing, quality, and the safety of barley and its related products.

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Barley Proteomics

Cited by 3 publications

References 93 publications

Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s

Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s

Cytoskeleton members, MVBs and the ESCRT-III HvSNF7s are putative key players for protein sorting into protein bodies during barley endosperm development

Application of Mass Spectrometry-Based Proteomics to Barley Research

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