Barley RIC171 interacts with RACB<i>in planta</i>and supports entry of the powdery mildew fungus

Schultheiß, Holger; Preuss, Jutta; Pircher, Thomas; Eichmann, Ruth; Hückelhoven, Ralph

doi:10.1111/j.1462-5822.2008.01167.x

Cited by 49 publications

(96 citation statements)

References 45 publications

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“…To validate these results, we used FRET measurement by fluorescence enhancement of CFP-CARACB after photobleaching of the YFP-MAGAP1 acceptor at the cell periphery, leading to similar results ( Figure 3D; for details, see Methods). This supports that MAGAP1 interacts with CARACB but not, or weakly, with the DN form of RACB and suggests an interaction of the proteins at the cell periphery, where active RACB is localized (Schultheiss et al, 2003(Schultheiss et al, , 2008.…”

Section: Racb Interacts With a Mt-associated Ropgap In Barleysupporting

confidence: 55%

“…Localization of GFP-MAGAP1 completely changed when we coexpressed CARACB, which is associated mainly with the plasma membrane (Schultheiss et al, 2003(Schultheiss et al, , 2008. With coexpression of CARACB, MT association of MAGAP1 was abolished.…”

Section: Magap1 Is Associated With Barley Mtsmentioning

confidence: 85%

“…Additionally, GFP-MAGAP1 was associated with cortical and perinuclear MTs when expressed alone but located at the cell periphery when coexpressed with CARACB ( Figure 5E). Because CARACB is associated with the plasma membrane (Schultheiss et al, 2003(Schultheiss et al, , 2008 and FRET signals were strongest at the cell periphery, data suggest that active RACB recruited MAGAP1 to the cell periphery. MAGAP1 is thus a previously undiscovered type of ROPGAP that associates with MTs when ROPs are inactive but is apparently released upon ROP activation for negative regulation.…”

Section: Racb Interacts With Magap1mentioning

confidence: 92%

“…Additionally, CARACB can interact with the barley CRIB motif protein RIC171 in planta and recruits red fluorescent DsRED-RIC171 to the plasma membrane. DsRED-RIC171 concentrates at sites of fungal entry, and overexpression of RIC171 supports accommodation of fungal haustoria, suggesting that local activity of ROPs is involved in susceptibility to fungal invasion (Schultheiss et al, 2008). However, although RACB has been established as a susceptibility factor in the barley-powdery mildew interaction, little is known about the regulation and the mechanism of RACB function in organization of the cytoskeleton and in susceptibility to powdery mildew.…”

Section: Introductionmentioning

confidence: 99%

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A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

et al. 2011

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Little is known about the function of host factors involved in disease susceptibility. The barley (Hordeum vulgare) ROP (RHO of plants) G-protein RACB is required for full susceptibility of the leaf epidermis to invasion by the biotrophic fungus Blumeria graminis f. sp hordei. Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal leaf cells and to form hairs on the root epidermis, suggesting that RACB is a common element of root hair outgrowth and ingrowth of haustoria in leaf epidermal cells. We further identified a barley MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN (MAGAP1) interacting with RACB in yeast and in planta. Fluorescent MAGAP1 decorated cortical microtubules and was recruited by activated RACB to the cell periphery. Under fungal attack, MAGAP1-labeled microtubules built a polarized network at sites of successful defense. By contrast, microtubules loosened where the fungus succeeded in penetration. Genetic evidence suggests a function of MAGAP1 in limiting susceptibility to penetration by B. graminis. Additionally, MAGAP1 influenced the polar organization of cortical microtubules. These results add to our understanding of how intact plant cells accommodate fungal infection structures and suggest that RACB and MAGAP1 might be antagonistic players in cytoskeleton organization for fungal entry.

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Section: Racb Interacts With a Mt-associated Ropgap In Barleysupporting

confidence: 55%

Section: Magap1 Is Associated With Barley Mtsmentioning

confidence: 85%

Section: Racb Interacts With Magap1mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

et al. 2011

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“…Additionally, in resemblance of PM-resident SNAREs, MLO and BAX Inhibitor-1 (see above), barley ROP-interactive CRIB (Cdc42/Rac-interactive binding) motif-containing protein (RIC) 171 has been found to accumulate underneath attempted fungal entry sites [67]. RICs are plant-specific proteins regulating RAC/ROPs by GTP-dependent interactions.…”

Section: Membrane Rafts and Tip Growthmentioning

confidence: 99%