Barley β-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts

Fusté, Noel P.; Guasch‐Vallés, Marta; Guillén, P; Anerillas, Carlos; Cemeli, Tània; Pedraza, Neus; Ferrezuelo, Francisco; Encinas, Mario; Moralejo, Marian; Garí, Eloi

doi:10.1016/j.carbpol.2019.01.090

Cited by 52 publications

(40 citation statements)

References 42 publications

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“…Cellular debris were removed by extensive washing with PBS. Then, the cells were treated with DMEM and 10% FBS containing glucan samples at different concentrations (10,20,50, and 100 µg mL −1 ). The cells were then incubated at 37 • C in a humidified 5% CO 2 atmosphere for 24 and 48 h. The scratch area was periodically imaged, at 0, 24, and 48 h acquired at 40× of magnification (Leica DMIL, Buccinasco (MI), Italy) in clear field.…”

Section: In Vitro Scratch Testmentioning

confidence: 99%

“…This latter capability is due to several bioactive compounds present in the fruit [8,9]. In recent years, glucans of oats and barley, in particular those ones with a β conformation, have just been studied in wound healing, showing multifunctional activities [8,10,11]. Glucans are present in various plant-origin foods and therefore can potentially be extracted from a variety of food matrices [12].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

et al. 2020

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Glucans possess a broad spectrum of biological activities. In this context, the present study was performed to isolate glucans from an Italian cultivar of Ziziphus jujuba Mill. at three different harvesting periods, in order to evaluate their effects on wound healing. The dry fruits were subjected to an alkaline extraction and then isolated glucans were purified by dialyzation. The crude and soluble samples were characterized by FT-IR and SEM analyses. Afterwards, total, α- and β-glucan content was measured using an enzymatic procedure. The results highlighted that the glucan amount increased as the maturation proceeded as well as the β-glucan percentage, which ranged from 48.2 at the first harvesting to 65.4 at the third harvesting. Furthermore, the effects of isolated glucans on the viability and migration of keratinocytes were evaluated using the in vitro MTT and scratch wound assays. The best proliferative effects on keratinocyte migration have been achieved with soluble glucans from third harvesting at 100 μM after 24 and 48 h (*** P < 0.001). The same treated group showed significant narrowing of the scratch area after 24 h and complete closure of the injury after 48 h. The findings highlighted the effectiveness of soluble glucans on regeneration of damaged skin.

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Section: In Vitro Scratch Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

et al. 2020

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“…Nowadays, β-glucans represent effective topical agents for the treatment of chronic wounds and burns due to the activation of the immune and cutaneous cells (see Table 3), which increase wound repair by enhancing the infiltration of macrophages and promote tissue granulation, collagen deposition, and reepithelialization based on inducing the proliferation and migration of keratinocytes and fibroblasts through specific receptors such as Dectin-1, CR3, or TLRs [132]. Barley β-glucan in vivo promotes the wound closure in mouse skin by promoting the migration and proliferation of human dermis fibroblasts [133].…”

Section: Cardiovascular Disease Preventionmentioning

confidence: 99%

“…β-Glucans can be used as candidates for the medication in the treatment of human chronic diseases [133]. β-Glucans have many bioactivities including antidiabetes; anticancer; antiobesity; anti-inflammation; immunomodulation; cardioprotection; lower cholesterol and lower blood pressure; improve bowel health, gastroprotection, and hepatoprotection; reduce chronic kidney disease and metabolic syndrome; prevent the risk of heart and cardiovascular diseases; and accelerate wound healing activities (Figure 1, Tables 2 and 3) [13,18,98,111,116,122,123,127,133,[143][144][145][146][147][148][149]. The major mechanisms of β-glucans of barley involve in the prevention of chronic diseases are as follows: β-glucans can interact with intestinal lipids and bile salt to reduce cholesterol levels and subsequently prevent diabetes, hypertension, cardiovascular disease, and metabolic syndrome [143].…”

Section: Major Mechanisms Of Functional Ingredients In Barleymentioning

confidence: 99%

“…Barley β-glucan not only has the gastroprotective effects by increasing the SOD and CAT activity, decreasing the gastric ulcer index, and increasing prostaglandin E2 and nitric oxide in laboratory rodents [122] but also affects lipid metabolism and SCFA production, lowering microbes in patients with metabolic syndrome [127] and improving chronic kidney disease by reducing the microbial-derived uremic toxin. Chronic consumption of barley β-glucans can decrease fatty liver by increasing small intestinal contents viscosity and improving glucose, lower glycated hemoglobin and relative kidney weights [129], strengthen the angiogenic ability of ROS-exposed endothelial cells for preventive heart disease [123], and accelerate the wound closure by promoting the migration and proliferation of human dermal fibroblasts [133].…”

Section: Major Mechanisms Of Functional Ingredients In Barleymentioning

confidence: 99%

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Molecular Mechanism of Functional Ingredients in Barley to Combat Human Chronic Diseases

Zeng

et al. 2020

Oxidative Medicine and Cellular Longevity

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Barley plays an important role in health and civilization of human migration from Africa to Asia, later to Eurasia. We demonstrated the systematic mechanism of functional ingredients in barley to combat chronic diseases, based on PubMed, CNKI, and ISI Web of Science databases from 2004 to 2020. Barley and its extracts are rich in 30 ingredients to combat more than 20 chronic diseases, which include the 14 similar and 9 different chronic diseases between grains and grass, due to the major molecular mechanism of six functional ingredients of barley grass (GABA, flavonoids, SOD, K-Ca, vitamins, and tryptophan) and grains (β-glucans, polyphenols, arabinoxylan, phytosterols, tocols, and resistant starch). The antioxidant activity of barley grass and grain has the same and different functional components. These results support findings that barley grain and its grass are the best functional food, promoting ancient Babylonian and Egyptian civilizations, and further show the depending functional ingredients for diet from Pliocene hominids in Africa and Neanderthals in Europe to modern humans in the world. This review paper not only reveals the formation and action mechanism of barley diet overcoming human chronic diseases, but also provides scientific basis for the development of health products and drugs for the prevention and treatment of human chronic diseases.

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pH‐Responsive β‐Glucans‐Complexed mRNA in LNPs as an Oral Vaccine for Enhancing Cancer Immunotherapy

Luo,

Ho,

Chiang

et al. 2024

Advanced Materials

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AbstractmRNA vaccines for cancer immunotherapy are commonly delivered using lipid nanoparticles (LNPs), which, when administered intravenously, may accumulate in the liver, potentially limiting their therapeutic efficacy. To overcome this challenge, the study introduces an oral mRNA vaccine formulation tailored for efficient uptake by immune cells in the gastrointestinal (GI) tract, known for its high concentration of immune cells, including dendritic cells (DCs). This formulation comprises mRNA complexed with β‐glucans (βGlus), a potential adjuvant for vaccines, encapsulated within LNPs (βGlus/mRNA@LNPs). The βGlus/mRNA complexes within the small compartments of LNPs demonstrate a distinctive ability to partially dissociate and reassociate, responding to pH changes, effectively shielding mRNA from degradation in the harsh GI environment. Upon oral administration to tumor‐bearing mice, βGlus/mRNA@LNPs are effectively taken up by intestinal DCs and local nonimmune cells, bypassing potential liver accumulation. This initiates antigen‐specific immune responses through successful mRNA translation, followed by drainage into the mesenteric lymph nodes to stimulate T cells and trigger specific adaptive immune responses, ultimately enhancing antitumor effects. Importantly, the vaccine demonstrates safety, with no significant inflammatory reactions observed. In conclusion, the potential of oral βGlus/mRNA@LNPs delivery presents a promising avenue in cancer immunotherapy, offering needle‐free and user‐friendly administration for widespread adoption and self‐administration.

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Barley β-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts

Cited by 52 publications

References 42 publications

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

Molecular Mechanism of Functional Ingredients in Barley to Combat Human Chronic Diseases

pH‐Responsive β‐Glucans‐Complexed mRNA in LNPs as an Oral Vaccine for Enhancing Cancer Immunotherapy

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