Due to the intrinsically complex non-equilbrium behavior of the constituents of active matter systems, a comprehensive understanding of their collective properties is a challenge that requires systematic bottom-up characterization of the individual components and their interactions. For self-propelled particles, an intrinsic complexity stems from the fact that the polar nature of the colloids necessitates that the interactions depend on positions and orientations of the particles, leading to a $2 d-1$ dimensional configuration space for each particle, in $d$ dimensions. Moverover, the interactions between such non-equilibrium colloids are generically non-reciprocal, which makes the characterization even more complex. Therefore, derivation of generic rules that enable us to predict the outcomes of individual encounters as well as the ensuing collective behavior will provide an important progress. While significant advances have been made on the theorical front, such systematic experimental characterizations using simple artificial systems with measurable parameters are scarce. Here, we study two different, contrasting types of colloidal microswimmers, which move in opposite directions and show distinctly different interactions. To facilitate the extraction of parameters, we introduce an experimental platform in which we confine them on a 1D track. Furthermore, we develop a theoretical model for interparticle interactions near a substrate, including both phoretic and hydrodynamic effects, which reproduces their behavior. For subsequent validation, we increase the degrees of freedom to two-dimensional motion and predict the resulting trajectories, finding remarkable agreement.
Our results may prove useful in characterizing the overall alignment behavior of interacting self-propelling active swimmer and may find application in guiding the design of active-matter systems involving phoretic and hydrodynamic interactions.