The promotion of breast self-examination (BSE) in developing countries is the main strategy for reducing breast cancer-related mortality. For this reason, determining the barriers to perform BSE for nurses, who play a key role in the health education of the society, should be a priority step. This research was conducted to determine the barriers to perform BSE for nurses. The sample of this descriptive, cross-sectional study consisted of 276 nurses working in an education and research hospital, agreed to participate in the study. The data of the study, which was conducted between January and June 2020, were collected using face-to-face interview method via questionnaire form and Champion Health Belief Model Scale (CHBMS). Number and percentage distribution, One Way ANOVA, Student t-test, Kruskal-Wallis, MannWhitney U test, Spearman Correlation and Multi-directional Regression Analysis were used in data analysis. The mean age of the nurses participating in the study was 32.56±9.07. 52.9% of the nurses are single and the majority (75.7%) are university graduates. 73.9% of the nurses perform BSE. The rate of those who perform BSE at the right time is 40.5%. The difference between the disability, trust, and health motivation sub-dimension mean scores of nurses who perform BSE and those who do not are statistically significant. In this study, according to the health belief model used to determine the barriers to nurses' BSE performance, disability perception scores were high, the factors that most affect the perception of disability were marriage, having children, the clinic which they work, breast cancer risk, age, working year and sensitivity, severity. It was determined that they had benefit, sense of trust and health motivation.