Background: Pyelonephritis as a life-threatening infection often leads to renal scarring, which presumably controls by minerals. Objectives: The aim of this study was pyelonephritis treatment by vitamin A in children. Methods: In the current study,106 cases from Imam Reza pediatric clinic and Amir-Kabir Hospital, considered a study group. Children divided into groups with or without urinary incontinence. Demographic data, clinical manifestations, and urinary biomarkers were evaluated and then statistically analyzed. Results: Our data showed that there is a statistical difference between study groups in terms of dysuria (P = 0.001), abdominal pain (P = 0.001), frequency (P = 0.003), incontinency (P = 0.001), urgency (P = 0.002), intermittency (P = 0.004) and fever (P = 0.002). Conclusions: It has been assumed that vitamin A as a therapeutic agent could be used in children with vesicoureteral reflux induced by Pyelonephritis and congenital anomalies.