Basal and exercise induced label-free quantitative protein profiling of m. vastus lateralis in trained and untrained individuals

Schild, Marius; Ruhs, Aaron; Beiter, Thomas; Zügel, Martina; Hudemann, Jens; Reimer, Anna; Krumholz-Wagner, Ilke; Wagner, Carola; Keller, Janine; Eder, Klaus; Krüger, Karsten; Krüger, Marcus; Braun, Thomas; Nieß, Andreas M.; Steinacker, Jürgen M.; Mooren, Frank C.

doi:10.1016/j.jprot.2015.03.028

Cited by 62 publications

(78 citation statements)

References 57 publications

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“…Apo A-I was not affected by acute exercise but showed a significant group effect with higher levels in the EET group. In line with this, the LC-MS/MS skeletal muscle analysis revealed elevated levels of key enzymes of the oxidative energy metabolism in the endurance-trained muscle and, therefore, the ability for enhanced muscular glucose utilization [19]. During intense endurance exercise, glucose is the major fuel for the exercising muscle mobilized from muscle and liver glycogen stores.…”

Section: Interplay Of Immune System and Metabolic Regulationsupporting

confidence: 53%

“…In a previous study, we analyzed skeletal muscle specimen from a subset of these individuals using an off-gel liquid chromatography tandem mass spectrometry (LC-MS/MS) approach. Proteome profiling revealed differences in various metabolic, especially mitochondrial proteins between endurance-trained and untrained individuals under resting conditions and in response to acute exercise [19]. In addition, a subgroup analysis of participants and variables was performed in connection with extracellular DNA traps and cell-free DNA evaluation in plasma [20].…”

Section: Eet ( = 19)mentioning

confidence: 99%

“…In the present study, the highest values of IL-6 for both groups were measured immediately after exercise with a more pronounced effect in the EET group. IL-6 has been demonstrated to provide metabolic effects, like enhanced glycogenolysis and fatty acid oxidation [15], which correspond to the elevated abundance of mitochondrial enzymes of the tricarboxylic acid and oxidative phosphorylation in the endurance-trained skeletal muscle [19] and, therefore, the possibility of utilizing more substrate for energy expenditure. IL-6 knockout mice showed an impaired carbohydrate and lipid metabolism and, in addition, were insensitive towards the metabolic effects of leptin despite elevated circulating plasma leptin level [44].…”

Section: Interplay Of Immune System and Metabolic Regulationmentioning

confidence: 99%

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The Effects of Training and Detraining on Metabolic Hormones in Rats

Jang¹,

Joo²

2017

Exerc Sci

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Acute physical exercise and repeated exercise stimuli affect whole-body metabolic and immunologic homeostasis. The aim of this study was to determine plasma protein profiles of trained (EET, = 19) and untrained (SED, = 17) individuals at rest and in response to an acute bout of endurance exercise. Participants completed a bicycle exercise test at an intensity corresponding to 80% of their VO 2max . Plasma samples were taken before, directly after, and three hours after exercise and analyzed using multiplex immunoassays. Seventy-eight plasma variables were included in the final analysis. Twenty-nine variables displayed significant acute exercise effects in both groups. Seven proteins differed between groups, without being affected by acute exercise. Among these A2Macro and IL-5 were higher in EET individuals while leptin showed elevated levels in SED individuals. Fifteen variables revealed group and time differences with elevated levels for IL-3, IL-7, IL-10, and TNFR2 in EET individuals. An interaction effect could be observed for nine variables including IL-6, MMP-2, MMP-3, and muscle damage markers. The proteins that differ between groups indicate a long-term exercise effect on plasma protein concentrations. These findings might be of importance in the development of exercise-based strategies in the prevention and therapy of chronic metabolic and inflammatory diseases and for training monitoring.

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Section: Interplay Of Immune System and Metabolic Regulationsupporting

confidence: 53%

Section: Eet ( = 19)mentioning

confidence: 99%

Section: Interplay Of Immune System and Metabolic Regulationmentioning

confidence: 99%

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The Effects of Training and Detraining on Metabolic Hormones in Rats

Jang¹,

Joo²

2017

Exerc Sci

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“…Although not discussed in detail, small noncoding RNA molecules such as microRNA are also important contributors to exercise-regulated posttranscriptional modulation and represent an exciting area for future investigation in epigenetic control of the exercise response (as systematically reviewed in Flowers et al 2015). (Schild et al 2015), myositis (Munters et al 2016), and type 2 diabetes (T2D) (Hussey et al 2013) relevant to muscle metabolic health has been uncovered using proteomics. Bioinformatics analysis of network adaptations has revealed important exercise variables underlying skeletal muscle (Padrao et al 2016) and heart (Ferreira et al 2015) plasticity.…”

Section: Omics and Exercise Research Tracksmentioning

confidence: 99%

Omics and Exercise: Global Approaches for Mapping Exercise Biological Networks

Hoffman

2017

Cold Spring Harb Perspect Med

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The application of global "-omics" technologies to exercise has introduced new opportunities to map the complexity and interconnectedness of biological networks underlying the tissue-specific responses and systemic health benefits of exercise. This review will introduce major research tracks and recent advancements in this emerging field, as well as critical gaps in understanding the orchestration of molecular exercise dynamics that will benefit from unbiased omics investigations. Furthermore, significant research hurdles that need to be overcome to effectively fill these gaps related to data collection, computation, interpretation, and integration across omics applications will be discussed. Collectively, a cross-disciplinary physiological and omics-based systems approach will lead to discovery of a wealth of novel exercise-regulated targets for future mechanistic validation. This frontier in exercise biology will aid the development of personalized therapeutic strategies to improve athletic performance and human health through precision exercise medicine.T he dynamic human physiological responses to exercise have been widely studied in the context of metabolism and mechanical stress. Health benefits of exercise in prevention, delay, and/or treatment of pathophysiology associated with metabolic disorders and aging are widely appreciated. However, the intricacies of molecular networks and biological mechanisms underlying how humans adapt to exercise and acquire health benefits are not fully understood. In response to perturbations in whole-body homeostasis induced by physical activity and exercise, biological networks are stimulated in various cell types and organs to manage systemic metabolic and mechanical demands (Hawley et al. 2014). Targeted, reductionist-based approaches have laid the foundation for our understanding of distinct biological mechanisms regulating acute versus repeated exercise adaptations using a range of experimental models from cells to animals and humans. However, the complexity and integrated nature of the whole-body exercise response warrants global unbiased systems approaches to unravel the interwoven networks underlying the benefits of exercise in health and disease.In this early stage of the exercise and omics revolution, there is a wealth of molecular information now within reach to help build on our understanding of human metabolism and exercise physiology. There is tremendous potential for omics approaches to fill critical gaps in our

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“…Another study involving 2D fluorescence difference gel electrophoresis (2DDIGE)-based analysis of human skeletal muscle proteome showed that the extensive remodeling of the mitochondrial proteome occurred after only seven days of exercise training [120]. Using FASP-based digestion method and OFFGEL fractionation, 3481 proteins were identified in human skeletal muscle; however, only 702 proteins could be identified in all samples [121]. Despite this, proteomics analysis of skeletal muscle from healthy endurance exercise-trained and untrained individuals showed clear differences in proteome profiles.…”

Section: Proteomics Application To Study Exercise Biologymentioning

confidence: 99%

Proteomics of Skeletal Muscle: Focus on Insulin Resistance and Exercise Biology

Deshmukh

2016

Proteomes

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Skeletal muscle is the largest tissue in the human body and plays an important role in locomotion and whole body metabolism. It accounts for ~80% of insulin stimulated glucose disposal. Skeletal muscle insulin resistance, a primary feature of Type 2 diabetes, is caused by a decreased ability of muscle to respond to circulating insulin. Physical exercise improves insulin sensitivity and whole body metabolism and remains one of the most promising interventions for the prevention of Type 2 diabetes. Insulin resistance and exercise adaptations in skeletal muscle might be a cause, or consequence, of altered protein expressions profiles and/or their posttranslational modifications (PTMs). Mass spectrometry (MS)-based proteomics offer enormous promise for investigating the molecular mechanisms underlying skeletal muscle insulin resistance and exercise-induced adaptation; however, skeletal muscle proteomics are challenging. This review describes the technical limitations of skeletal muscle proteomics as well as emerging developments in proteomics workflow with respect to samples preparation, liquid chromatography (LC), MS and computational analysis. These technologies have not yet been fully exploited in the field of skeletal muscle proteomics. Future studies that involve state-of-the-art proteomics technology will broaden our understanding of exercise-induced adaptations as well as molecular pathogenesis of insulin resistance. This could lead to the identification of new therapeutic targets.

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Basal and exercise induced label-free quantitative protein profiling of m. vastus lateralis in trained and untrained individuals

Cited by 62 publications

References 57 publications

The Effects of Training and Detraining on Metabolic Hormones in Rats

The Effects of Training and Detraining on Metabolic Hormones in Rats

Omics and Exercise: Global Approaches for Mapping Exercise Biological Networks

Proteomics of Skeletal Muscle: Focus on Insulin Resistance and Exercise Biology

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