BASAL BODIES, BUT NOT CENTRIOLES, IN <i>NAEGLERIA </i>

Fulton, Chandler; Dingle, Allan D.

doi:10.1083/jcb.51.3.826

Cited by 135 publications

(101 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Gould and Borisy [1977] or more recently Afonso et al [2007] have indicated that retroviruses target the centrosome (and sometimes hijack centrosomal functions), which is consistent with centriolar origin from a retrovirus. Centrioles self-assemble de novo in ameboflagellates [Fulton and Dingle, 1971] and during male gametogenesis in aquatic ferns and in Gingko, where they disappear after fertilization. In Chlamydomonas, centrioles can form either by a templated pathway with pre-existing centrioles or by de novo assembly that is normally repressed when pre-existing centrioles are present [Marshall et al, 2001].…”

Section: Viral-like Replication and Self-assemblymentioning

confidence: 99%

Evolution and persistence of the cilium

Satir

Guerra

Bell

2007

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

The origin of cilia, a fundamental eukaryotic organelle, not present in prokaryotes, poses many problems, including the origins of motility and sensory function, the origins of nine-fold symmetry, of basal bodies, and of transport and selective mechanisms involved in ciliogenesis. We propose the basis of ciliary origin to be a self-assembly RNA enveloped virus that contains unique tubulin and tektin precursors. The virus becomes the centriole and basal body, which would account for the self-assembly and self-replicative properties of these organelles, in contrast to previous proposals of spirochaete origin or endogenous differentiation, which do not readily account for the centriole or its properties. The viral envelope evolves into a sensory bud. The host cell supplies the transport machinery and molecular motors to construct the axoneme. Polymerization of cytoplasmic microtubules in the 9 1 0 axoneme completes the 9 1 2 pattern. Cell Motil. Cytoskeleton 64: 906-913, 2007. ' 2007

show abstract

Section: Viral-like Replication and Self-assemblymentioning

confidence: 99%

Evolution and persistence of the cilium

Satir

Guerra

Bell

2007

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…Switching between flagella-based swimming motility and actin-based gliding motility might be an ancient strategy for adapting to different environments. For instance, the excavate Naegleria gruberii can interconvert between a flagellate and an amoeba depending on whether it is placed in a liquid environment or associated with a solid substrate [64,[69][70][71][72]. In Naegleria flagellates, the two centrioles anchor microtubule roots that contribute to cell morphology in association with a rigid cortex of actomyosin.…”

Section: (A) the Amorphean Ancestormentioning

confidence: 99%

Exploring the evolutionary history of centrosomes

Azimzadeh

2014

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

The centrosome is the main organizer of the microtubule cytoskeleton in animals, higher fungi and several other eukaryotic lineages. Centrosomes are usually located at the centre of cell in tight association with the nuclear envelope and duplicate at each cell cycle. Despite a great structural diversity between the different types of centrosomes, they are functionally equivalent and share at least some of their molecular components. In this paper, we explore the evolutionary origin of the different centrosomes, in an attempt to understand whether they are derived from an ancestral centrosome or evolved independently from the motile apparatus of distinct flagellated ancestors. We then discuss the evolution of centrosome structure and function within the animal lineage.

show abstract

“…FIGURE 13 Ouchterlony immunodiffusion test of antiserum to rootlet protein . The antiserum to rootlet protein, well B, was assayed against all the SDS-solubilized proteins of a typical rootlet preparation, well A .…”

Section: Rootlet Localization In Vivomentioning

confidence: 99%

“…The rapid, controlled, and highly reproducible cellular transformation of Naegleria has been promoted as a model for the study of eukaryotic cell differentiation (4,11), and the sequence of development of both flagella and basal bodies has been elucidated using this model (5,12,13) . Because organelle differentiation can be precisely timed during flagellate differentiation (4,12), and the organelle number can be experimentally manipulated (3,6), a study of rootlet development correlated with basal body and flagellum development also becomes feasible in this system .…”

mentioning

confidence: 99%

Isolation, ultrastructure, and protein composition of the flagellar rootlet of Naegleria gruberi.

Larson¹,

Dingle²

1981

The Journal of Cell Biology

View full text Add to dashboard Cite

Attached to the basal bodies of Naegleria gruberi flagellates is a striated rootlet or rhizoplast . The rootlet-basal body complex has been isolated by Triton X-100 lysis of deflagellated cells and differential centrifugation through a 25% glycerol medium . Rootlets isolated from mature flagellates are -13 p,m long but vary from 8 to 15 ftm in length : they taper at both ends from a maximum width of -0 .25 p,m in the vicinity of the basal bodies . They are highly stable during isolation but can be solubilized by urea, high salt, low pH, or detergent (Sarkosyl) . Partial dissociation of rootlets with 1 M urea reveals that they are composed of filaments, -5 nm diameter, associated in linear fashion to yield the characteristic 21-nm cross-banded appearance . Differential solubilization of rootlets and their associated contaminants allowed identification of a major rootlet protein, comprising at least 50% of any purified rootlet preparation, with an apparent subunit molecular weight of 170,000. The localization of rootlets in situ by indirect immunofluorescence using a specific antibody directed against the purified rootlet protein demonstrated unequivocally that this 170,000-dalton protein is an organelle component.The flagella of eukaryotic cells are generally found as part of a flagellar apparatus-an interconnected assemblage of basal body, flagellum, and a striated rootlet fiber or rhizoplast . Likewise, cilia, basal bodies, and one or more rootlets often combine to constitute what could be called a "ciliary apparatus" in ciliated cells . Although flagellated and ciliated cells lacking rootlets are known, and even common (e .g., sperm cells, certain mammalian ciliated epithelia), some form of rootlet structure is attached to the basal bodies of most cilia and flagella (7,8,30) . Despite intense current interest in the structure, function, and morphogenesis of flagella and their basal bodies, virtually no attention has been paid to the other major component of the flagellar or ciliary apparatus, the rootlet. References to flagellar rootlets have most often been restricted to ultrastructural descriptions and to speculation about possible roles in motility (5,9,15,16,19,26,28,34,38) .Electron micrographs of rootlets generally reveal bundles of 4-to 5-nm-diameter filaments aggregated in a paracrystalline array to produce fibrous, cross-striated organelles reminiscent of collagen fibrils (9, 26, 28, 37) . The cross-striations form one major repeating unit that may be further divided into -13 intraperiod sub-bands (28, 38) . Reported widths for this major repeat unit range from 12 to 78 nm (34,35) . Though most authors describe a constant banding pattern, at least three reports have commented upon the variations in rootlet period-424 icity (15,32,34) and have inferred a possible physiological role for the altered states . The idea that the rootlet is a contractile element of the flagellar apparatus which may play an active role in modifying the flagellar beat is supported by the following : the recent demonst...

show abstract

BASAL BODIES, BUT NOT CENTRIOLES, IN NAEGLERIA

Cited by 135 publications

References 45 publications

Evolution and persistence of the cilium

Evolution and persistence of the cilium

Exploring the evolutionary history of centrosomes

Isolation, ultrastructure, and protein composition of the flagellar rootlet of Naegleria gruberi.

Contact Info

Product

Resources

About