Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium

Aida, Junko; Izumiyama-Shimomura, Naotaka; Nakamura, Kenichi; Ishikawa, Naoshi; Poon, Steven S.S.; Kammori, Makoto; Sawabe, Motoji; Arai, Tomio; Matsuura, M.; Fujiwara, Mutsunori; Kishimoto, Hiroshi; Takubo, Kaiyo

doi:10.1016/j.exger.2008.06.001

Cited by 49 publications

(84 citation statements)

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“…Using quantitative fluorescence in situ hybridization (Q-FISH) and our originally developed software, Tissue Telo, employing the telomere-to-centromere ratio (TCR) or normalized TCR (NTCR), we have confirmed the telomere length distributions of different cell types in the tongue [2], stomach [3], breast [4], and esophagus [5]. We have also demonstrated that alcoholics show reduced telomere length in the esophageal epithelium [6].…”

Section: Introductionmentioning

confidence: 93%

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Aida

Aida²,

Hasegawa

et al. 2015

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Background: Telomeres are repetitive DNA sequences located at the ends of chromosomes. Chromosomal and genomic instability due to telomere dysfunction has been known to play an important role in the carcinogenesis of some organs. Objectives: The aim of this study was to examine the correlation between smoking and the telomere length of human bronchial epithelial cells in individuals with and without lung cancer. Patients and Methods: We examined 68 non-lung cancer adult autopsy cases and 24 surgically resected cases of lung squamous cell carcinoma. Telomere lengths of the basal cells of bronchial epithelium were measured using the tissue quantitative fluorescence in situ hybridization method and were expressed in normalized telomere-to-centromere ratios (NTCRs). Results: The autopsied individuals included 27 current smokers (CuS), 33 never-smokers (NeS), and 8 ex-smokers (ExS). The NTCRs in the central bronchi of CuS, NeS, and ExS were 1.515, 1.372, and 1.204, respectively. The bronchial epithelial telomeres of CuS were significantly longer than those of non-CuS (NeS + ExS). When the analysis was conducted separately for females and males, a significant difference between CuS and NeS + ExS was recognized only for males. The NTCRs of the bronchial epithelium of lung cancer cases and lung cancer tissue are 1.514 and 1.385, respectively. Conclusions: Our findings suggest that smoking causes telomeric elongation in the bronchial epithelium. Therefore, it appears that the mechanism of carcinogenesis in smoking-related carcinomas may differ from that of many other carcinomas in which genetic instability due to aging-related telomeric shortening is assumed to play a role.

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Section: Introductionmentioning

confidence: 93%

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Aida

Aida²,

Hasegawa

et al. 2015

Respiration

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“…Based on the fluorescence intensities, the telomere length index was determined from the telomere-centromere ratio (TCR) of each cell in the sample using our own analytical software package, TissueTelo Ver.3.1 (Takubo et al 2010b;Tamura et al 2014). To normalize for sample preparation, the TCR was also extracted from a control cell-block section placed on the same slide consisting of sectioned cells from a cultured cell strain, TIG-1, which is a human fetal lung-derived fibroblast-like cell strain established at our facility (Aida et al, 2008). TIG-1 is known to stop dividing after a certain number of subcultures (Ohashi et al, 1980), and its telomere length has been determined by Southern blotting.…”

Section: Subjectsmentioning

confidence: 99%

Telomere attrition in beta and alpha cells with age

Tamura

Izumiyama-Shimomura

Kimbara

et al. 2016

AGE

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We have reported telomere attrition in β and α cells of the pancreas in elderly patients with type 2 diabetes, but it has not been explored how the telomere lengths of these islet cells change according to age in normal subjects. To examine the telomere lengths of β and α cells in individuals without diabetes across a wide range of ages, we conducted measurement of the telomere lengths of human pancreatic β and α cells obtained from 104 autopsied subjects without diabetes ranging in age from 0 to 100 years. As an index of telomere lengths, the normalized telomere-centromere ratio (NTCR) was determined for β (NTCRβ) and α (NTCRα) cells by quantitative fluorescence in situ hybridization (Q-FISH). We found NTCRβ and NTCRα showed almost the same levels and both decreased according to age (p < 0.001 for both). NTCRs decreased more rapidly with age and were more widely distributed (p = 0.036 for NTCRβ, p < 0.001 for NTCRα) in subjects under 18 years of age than in subjects over 18 years. There was a positive correlation between NTCRβ and NTCRα only among adult subjects (p < 0.001). In conclusion, the telomeres of β and α cells become shortened with normal aging process.

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“…"Stromal cells", i.e. lymphocytes, endothe-lium and fibroblasts, have relatively constant telomere length in aging than epithelial cells, [21]. However, any method using admixed stromal cells as a control would not be reproducible, and the telomere lengths of stromal cells would be influenced by inflammation or regeneration factors, making them unsuitable as an internal control.…”

Section: Q-fish Methods For Telomere Measurement Using Tissue Sectionsmentioning

confidence: 99%

“…We demonstrated different telomere lengths and different annual reduction rates in each group of cells [21]- [23]. In human oral or esophageal carcinoma, shortened telomeres were present in the non-carcinomatous background mucosa showing chromosomal instability (Figure 3) [24]- [26], and shortened telomeres were also present in precancerous lesions such as oral leukoplakia [27] or actinic keratosis of the skin [6].…”

Section: Our Methodology For Q-fish and Image Analysis For Telomere Mmentioning

confidence: 99%

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Determination of Telomere Length by the Quantitative Fluorescence <i>in Situ</i> Hybridization (Q-FISH) Method

Aida¹,

Izumiyama-Shimomura²,

Nakamura³

et al. 2014

AJAC

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Telomeres are nucleoprotein complexes located at the ends of eukaryotic chromosomes and are shorten with aging or various causes. Shortened telomere plays an important role for chromosomal instability in carcinogenesis, or a number of diseases in relation to aging. FISH using peptide-nucleic acid (PNA) probe is suitable for analyzing telomeres, because telomere is a part of DNA molecule and localized in chromosomal end in loop shape structure (T-loop). We introduce our telomere analysis for tissue sections and cultured cells in this paper. On the tissue sections, we analyze telomere length with telomere intensity to centromere intensity ratio (TCR), centromere as an inner control. In the cultured cells, we analyze telomere lengths on each chromosome with karyotyping. Those Q-FISH methods by PNA probe are an accurate and reliable tool for research on telomere lengths in various conditions in biology.

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Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium

Cited by 49 publications

References 28 publications

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Telomere attrition in beta and alpha cells with age

Determination of Telomere Length by the Quantitative Fluorescence <i>in Situ</i> Hybridization (Q-FISH) Method

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Basal cells have longest telomeres measured by tissue Q-FISH method in lingual epithelium

Cited by 49 publications

References 28 publications

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Telomere attrition in beta and alpha cells with age

Determination of Telomere Length by the Quantitative Fluorescence &lt;i&gt;in Situ&lt;/i&gt; Hybridization (Q-FISH) Method

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Determination of Telomere Length by the Quantitative Fluorescence <i>in Situ</i> Hybridization (Q-FISH) Method