Base Composition of Deoxyribonucleic Acid from Bovine T-Mycoplasmas

Howard, C.J.; Gourlay, R. N.; Garwes, D. J.; Pocock, D. H.; Collins, Jacqueline

doi:10.1099/00207713-24-3-373

Cited by 10 publications

(4 citation statements)

References 5 publications

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“…Thus, although serologically heterogeneous, the bovine ureaplasmas seem to comprise a group of organisms with many common antigens but which, as a group, are distinct from the human strains comprising the species U. urealyticum. This finding, together with the earlier demonstration that the guanine and cytosine contents of U. urealyticum and the bovine ureaplasmas differ to an extent that denotes the existence of two distinguishable populations (3,7), indicates that the bovine ureaplasmas should be regarded as a group distinct from U.…”

Section: Discussionmentioning

confidence: 55%

Serological Studies with Bovine Ureaplasmas (T-Mycoplasmas)

Howard

Gourlay

Collins

1978

International Journal of Systematic Bacteriology

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Seventy-seven recently isolated bovine ureaplasma (T-mycoplasma) strains were compared by the growth inhibition test with previously reported strains. The complex serological nature of the group was confirmed. Only five of the fresh isolates did not react with antisera to the eight representative bovine strains proposed previously. The suggestion that the serological diversity of the group might be represented by a small number of strains has thus been confirmed. Eleven strains are proposed as representatives of this serological diversity. No common antigens were shown between Ureaplasma urealyticum, the ureaplasmas of human origin, and the bovine isolates.Ureaplasmas, mycoplasmas with urease activity previously called T-mycoplasmas (12), have been isolated from a variety of diseased and apparently nondiseased bovine tissues. The anatomical sites in cattle from which these mycoplasmas have been isolated include eyes, the urogenital tract, and respiratory tract (13). To determine whether some relationship exists between disease and the presence of ureaplasmas, it would be useful to have a means of serologically identifying strains. Reported studies (5, 6, 9) indicate that bovine ureaplasmas are serologically heterogeneous. However, it has been suggested that the serological diversity of the group could be represented by a small number of strains which possess between them the spectrum of antigens synthesized by the group (6). To test this suggestion and to study further the serology of the bovine ureaplasmas, a number of fresh isolates were collected and examined to determine how many of them showed serological cross-reactions with the eight bovine strains previously put forward as representing the group. MATERIALS AND METHODS Media.The broth used to culture ureaplasmas, designated U4, consisted of Hanks balanced salt solution (Wellcome, Beckenham, England) (lox concentrate), 4 ml , Hartley digest broth, 20 ml; fetal calf serum (Flow Laboratories, Irvine, Calif.), 15 ml; yeast extract (Distillers Co., Morden, England) (25% [wt/vol] aqueous extract), 10 ml; 1% phenol red, 0.2 ml, 20% urea, 0.25 ml , 5% thallium acetate, 0.5 ml; magnesium sulfate (250 pg/ml), 1 ml; benzylpenicillin (Glaxo Ltd., Greenford, England) (200,000 U/ml), 0.5 ml. Glass-distilled double-deionized water was added to bring the volume to 100 ml, and the pH was adjusted to 6.0 to 6.2 by the addition of N-hydrochloric acid.The solid medium consisted of: Hanks buffered salt solution (lox concentrate), 4 ml, agarose (Miles Labs, Slough, England), 0.8 g; N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (Sigma Chemical Co., St.Louis, Mo.), 1.19 g; Hartley broth, 20 d, glass-distilled water, 44 ml. This mixture was sterilized by autoclaving at 10 lb/in2 for 10 min. After cooling to 56°C the following were added fetal calf serum, 20 ml , 1% putrescine, 1 ml; 0.9% L-cysteine, 1 ml; and yeast extract, penicillin, thallium acetate, and magnesium sulfate at the concentrations noted above. The final pH of the solid medium was about 6.2. Cultures on soli...

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Section: Discussionmentioning

confidence: 55%

Serological Studies with Bovine Ureaplasmas (T-Mycoplasmas)

Howard

Gourlay

Collins

1978

International Journal of Systematic Bacteriology

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“…In a previous study, the guanine plus cytosine contents of the deoxyribonucleic acids from bovine strains were shown to be different from those of the eight serovars of U. urealyticum, indicating that the bovine and human isolates existed as two distinct populations and that the two groups of organisms should not be regarded as belonging to the same species (5,6).…”

Section: 7)mentioning

confidence: 96%

Polyacrylamide Gel Electrophoretic Comparison of the Polypeptides from Ureaplasmas Isolated from Cattle and Humans

Howard

Pocock

Gourlay

1981

International Journal of Systematic Bacteriology

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“…In an attempt to establish distinguishing features, several studies were published comparing morphological characteristics and protein band profiles, by electrophoresis, from T-strains isolated from cattle with human isolates ( 52 , 53 ). Serological tests showed differences between strains; however, the differences were not sufficiently marked to establish differences between species ( 53 , 54 ).…”

Section: Ureaplasma Diversummentioning

confidence: 99%

“…The confirmation was detailed in the DNA composition and the guanine plus cytosine (G + C) content. The human T-strain presented C+G 27.4 mol% ( 55 ) and bovine T-strains had 29.3% ( 52 ). Other T-strains were isolated from different animals including sheep and goats and the molecular methods allowed for these to be differentiated from bovine isolates ( 53 , 56 ).…”

Section: Ureaplasma Diversummentioning

confidence: 99%

A Review of Ureaplasma diversum: A Representative of the Mollicute Class Associated With Reproductive and Respiratory Disorders in Cattle

et al. 2021

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The Mollicutes class encompasses wall-less microbes with a reduced genome. They may infect plants, insects, humans, and animals including those on farms and in livestock. Ureaplasma diversum is a mollicute associated with decreased reproduction mainly in the conception rate in cattle, as well as weight loss and decreased quality in milk production. Therefore, U. diversum infection contributes to important economic losses, mainly in large cattle-producing countries such as the United States, China, Brazil, and India. The characteristics of Mollicutes, virulence, and pathogenic variations make it difficult to control their infections. Genomic analysis, prevalence studies, and immunomodulation assays help better understand the pathogenesis of bovine ureaplasma. Here we present the main features of transmission, virulence, immune response, and pathogenesis of U. diversum in bovines.

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Base Composition of Deoxyribonucleic Acid from Bovine T-Mycoplasmas

Cited by 10 publications

References 5 publications

Serological Studies with Bovine Ureaplasmas (T-Mycoplasmas)

Serological Studies with Bovine Ureaplasmas (T-Mycoplasmas)

Polyacrylamide Gel Electrophoretic Comparison of the Polypeptides from Ureaplasmas Isolated from Cattle and Humans

A Review of Ureaplasma diversum: A Representative of the Mollicute Class Associated With Reproductive and Respiratory Disorders in Cattle

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