The laboratory in colleges and universities is an important place to carry out teaching and scientific research activities. The purpose of laboratory safety education is to continuously improve the experimental safety awareness of teachers and students, effectively enhance the initiative of safety precautions, safeguard their own and collective safety, and maintain the normal order of experiments in colleges and universities.Aiming at the problems existing in laboratory safety education, such as simplification of educational methods, formalization of educational process, insufficient safety culture construction and imperfect safety education system, and following the basic principles of laboratory safety education in colleges and universities, this paper puts forward the reform path of laboratory safety education in colleges and universities. In-depth promotion of laboratory "intrinsic safety" education, extensive publicity and training of laboratory safety education, rich practical activities of laboratory safety education, construction of safety education platform based on WeChat applet, and integration of the concept of "course thinking and politics" into laboratory safety education.