Baseline sensitivity of Fusarium graminearum from wheat, corn, dry bean and soybean to pydiflumetofen in Michigan, USA

Breunig, Mikaela; Chilvers, Martin I.

doi:10.1016/j.cropro.2020.105419

Cited by 25 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17,20,24,25,28 It shows strong antifungal activity against many fungal pathogens, including Didymella bryoniae, S. sclerotiorum, Botrytis cinerea, F. asiaticum and Fusarium graminearum. 17,20,24,25,29,[35][36][37] Pydiflumetofen resistance risks have been characterized in some plant pathogens, and point mutations on the subunits SdhB, SdhC, and SdhD of SDH could result in fungicide resistance in those fungi. 17,24,25,37 In the current study, we found that pydiflumetofen has excellent antifungal activity against M. oryzae, and could be used as the potential fungicides to control the rice blast disease.…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Liu

Jun

Jiang

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Rice (Oryza sativa) is an important cereal crop around the world, and has constantly been threaten by the most destructive fungus Magnaporthe oryzae. Pydiflumetofen, a novel succinate dehydrogenase inhibitor (SDHI), is currently being used for controlling various fungal diseases. However, the potential resistance risk of M. oryzae to pydiflumetofen has remained unclear to date, and finding the resistance mechanism is critical for the usage of this fungicide.RESULTS: The M. oryzae strain Guy11 is sensitive to pydiflumetofen, with EC 50 value of 1.24 ∼g mL −1 . 58 pydiflumetofenresistant (PR) mutants were obtained through pydiflumetofen-induced spontaneous mutation, with a mean EC 50 value >500 ∼g mL −1 , and the resistance factor (RF) >400. The PR mutants displayed positive cross-resistance to carboxin, but were more sensitive to fluopyram. Sequencing analysis showed that all PR mutants presented a cytosine-to-thymine transition at nucleotide position +1218, resulting in a replacement of histidine 245 by tyrosine (H245Y) on MoSdhB. The mutation of MoSdhB exhibited strong resistant phenotype, but no detectable growth deficits in fungal development, including vegetative growth and pathogenicity of M. oryzae. An allele-specific PCR for rapid detection of the H245Y mutants was established in M. oryzae.CONCLUSION: The M. oryzae is sensitive to pydiflumetofen, and shows a medium to high resistance risk to pydiflumetofen. A point mutation of MoSdhB (H245Y) is responsible for the fungal resistance to pydiflumetofen in M. oryzae.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Liu

Jun

Jiang

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Evaluation of baseline in vitro sensitivity to pydiflumetofen is essential to determine the EC 50 value of the fungicide (Breunig & Chilvers, 2021). Typically, the EC 50 is important in advising on growers' management decisions.…”

Section: Discussionmentioning

confidence: 99%

“…This study focused on assessing a newly developed SDHI seed treatment, pydiflumetofen, on blackleg disease. Pydiflumetofen is an N‐methoxy‐(phenyl‐ethyl)‐pyrazole‐carboxamide with a broad spectrum of activity (Breunig & Chilvers, 2021). This product was developed by Syngenta specifically targeting soybean sudden death syndrome caused by Fusarium virguliforme , bakanae in rice caused by Fusarium fujikuroi and blackleg disease in canola.…”

Section: Introductionmentioning

confidence: 99%

“…As Bai et al (2021) showed, pydiflumetofen is highly effective against the growth and sporulation of F. fujikuroi attacks on rice. Furthermore, Breunig and Chilvers (2021) showed that pydiflumetofen could be used in fusarium head blight management in wheat and maize. This study was undertaken to assess the efficacy of the SDHI fungicide pydiflumetofen as a seed treatment controlling seedborne and airborne infections of the blackleg pathogen under controlled and field conditions compared to other available fungicides and its potential to be added as a new tool to the blackleg management toolbox.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Pydiflumetofen: An SDHI seed‐applied fungicide, a potential tool for the canola‐blackleg management toolbox

et al. 2022

View full text Add to dashboard Cite

Canola (Brassica napus), as the world's second most important oilseed crop, is the most profitable farm crop for Canadian farmers, providing more than 207,000 Canadian jobs and $12 billion in wages across the country. Canada exports 90% of canola seeds, oil and meal produced annually to foreign markets (Canola Council of Canada, 2020).Blackleg disease caused by the actinomycete fungus Leptosphaeria maculans (anamorph: Phoma lingam) is one of the most destructive pathogens found in canola in North America, Australia, Europe and many other regions around the world .This pathogen can reduce yield by up to 80%, depending on the susceptibility of the cultivar grown (Marcroft et al., 2004). This fatal disease can be managed by an integrated approach using crop rotation, resistant cultivars, resistance (R)-gene rotation and fungicide application, while genetic resistance of the host is the mainstay

show abstract

“…18 Till now, pydiflumetofen is still the unique kind of SDHIs to effectively control the scab disease on corn and wheat. 19 traditional carboxamide bridge with other groups could be a promising design strategy for novel SDHIs. 20 Natural products (NPs) have a long history as a promising source of lead compounds and design inspirations for new crop protection agents.…”

Section: ■ Introductionmentioning

confidence: 99%

Novel Camphor Sulfonohydrazide and Sulfonamide Derivatives as Potential Succinate Dehydrogenase Inhibitors against Phytopathogenic Fungi/Oomycetes

Yang

Sun

Jin

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

To discover novel fungicidal agrochemicals for treating wheat scab, 39 novel camphor sulfonohydrazide/sulfonamide derivatives 4a−4t and 6a−6s were designed and synthesized. In the in vitro antifungal/antioomycete assay, compounds 4g, 4n, and 4o displayed significant inhibitory activities against Fusarium graminearum, Botryosphaeria dothidea, and Phytophthora capsici. Among them, 4n exhibited the best antifungal activity against F. graminearum with an EC 50 value of 0.41 mg/L, which was at the same level as that of pydiflumetofen. The in vivo experiment revealed that 4n presented excellent protective and curative efficacy toward F. graminearum. In the antifungal mechanism study, 4n could increase the cell membrane permeability and reduce the exopolysaccharide and ergosterol content of F. graminearum. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses revealed that 4n could significantly damage the surface morphology and the cell ultrastructure of mycelia to interfere with the growth of F. graminearum. Furthermore, 4n exhibited potent succinate dehydrogenase (SDH) inhibitory activity in vitro with an IC 50 value of 3.94 μM, which was equipotent to pydiflumetofen (IC 50 = 4.07 μM). The molecular dynamics simulation and docking study suggested that compound 4n could well occupy the active site and form strong interactions with the key residues of SDH. The above-mentioned results demonstrated that the title camphor sulfonohydrazide/sulfonamide derivatives could be promising lead compounds for further succinate dehydrogenase inhibitor (SDHI) fungicide development.

show abstract

Baseline sensitivity of Fusarium graminearum from wheat, corn, dry bean and soybean to pydiflumetofen in Michigan, USA

Cited by 25 publications

References 23 publications

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Pydiflumetofen: An SDHI seed‐applied fungicide, a potential tool for the canola‐blackleg management toolbox

Novel Camphor Sulfonohydrazide and Sulfonamide Derivatives as Potential Succinate Dehydrogenase Inhibitors against Phytopathogenic Fungi/Oomycetes

Contact Info

Product

Resources

About