2021
DOI: 10.3390/cells10113163
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BASHY Dye Platform Enables the Fluorescence Bioimaging of Myelin Debris Phagocytosis by Microglia during Demyelination

Abstract: Multiple sclerosis (MS) is a demyelinating disease of the central nervous system that is characterized by the presence of demyelinated regions with accumulated myelin lipid debris. Importantly, to allow effective remyelination, such debris must be cleared by microglia. Therefore, the study of microglial activity with sensitive tools is of great interest to better monitor the MS clinical course. Using a boronic acid-based (BASHY) fluorophore, specific for nonpolar lipid aggregates, we aimed to address BASHY’s a… Show more

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Cited by 11 publications
(6 citation statements)
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“…In the context of innovative fluorescent organoboron architectures [1,2,13–22] we developed recently the modular platform of boronic‐acid derived salicylidenehydrazone (BASHY) complexes [23,24] . These fluorescent dyes show an interesting combination of an intramolecular charge‐transfer (ICT) mechanism [23] and cyanine‐like behavior [25,26] and have revealed promising potential for bioimaging applications [23,26–29] . The dyes are generated in a synthetically flexible and robust multicomponent reaction, [23,26] opening manifold possibilities for the electronic manipulation of the BASHY skeleton [24] …”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…In the context of innovative fluorescent organoboron architectures [1,2,13–22] we developed recently the modular platform of boronic‐acid derived salicylidenehydrazone (BASHY) complexes [23,24] . These fluorescent dyes show an interesting combination of an intramolecular charge‐transfer (ICT) mechanism [23] and cyanine‐like behavior [25,26] and have revealed promising potential for bioimaging applications [23,26–29] . The dyes are generated in a synthetically flexible and robust multicomponent reaction, [23,26] opening manifold possibilities for the electronic manipulation of the BASHY skeleton [24] …”
Section: Introductionmentioning
confidence: 99%
“…[23,24] These fluorescent dyes show an interesting combination of an intramolecular charge-transfer (ICT) mechanism [23] and cyanine-like behavior [25,26] and have revealed promising potential for bioimaging applications. [23,[26][27][28][29] The dyes are generated in a synthetically flexible and robust multicomponent reaction, [23,26] opening manifold possibilities for the electronic manipulation of the BASHY skeleton. [24] Having previously established that the fluorescence properties of BASHY dyes are founded on the "horizontal" donor-πacceptor (D-π-A) architecture (red D and blue A in Figure 1) and its π-conjugation length, [23,24,26] we herein focus our attention on the electron-donor substitution at the "vertical" positions (green and cyan in Figure 1), principally using NEt 2 .…”
Section: Introductionmentioning
confidence: 99%
“…When indicated, cells were transfected 24h after seeding with mammalian expression plasmids to visualize Golgi (EYFP-Golgi7, Addgene plasmids # 56590), using JetPRIME transfection reagent (Polyplus transfection, Illkirch, France) at 1:3 transfection ratios. HMC3 and HMC3 Sacs-/- cells were incubated with 1mg/ml of myelin debris previously stained with BASHY probe as described (Pinto et al, 2021) for 1h to mimic microglia neuroprotective ability to remove debris, or with 0.0025% (v/v) 1 mm zymogen-coated fluorescent latex beads (Sigma Chemical) for 75 min to mimic microglia reactivity against infections. Mitochondria were visualized by incubating cells with Mitoview TM Fix 640 (Biotium; Fremont, CA, USA) for 2h before imaging.…”
Section: Methodsmentioning
confidence: 99%
“…Nonetheless, although the presence of myelin-enriched microglia is a feature of MS active demyelinating lesions, the question of why remyelination fails in MS patients remains. To better elucidate the underlying mechanisms, we recently developed a probe, a modular fluorescent platform based on boronic acid salicylidenehydrazone complexes (BASHY), that is able to target myelin phagocytosing microglia in ex vivo cerebellar organotypic cultures and in vivo EAE cerebellum (Pinto et al, 2021). Also, in line with this and to further understand why remyelination fails in MS, Gesine Saher recently published a study with significant impact unveiling a link between the cholesterol metabolism in microglia and the process of myelin repair.…”
Section: Remyelination Ability During Multiple Sclerosismentioning
confidence: 99%