Basic and applied research on multiple aminoglycoside antibiotic resistance of actinomycetes: an old-timer's recollection

Hotta, Kunimoto

doi:10.1093/jimb/kuab059

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Actinomycetes are a diverse group of microorganisms that are rich in valuable chemical compounds. For example, the antibiotics they produce are critical for the protection of plants, animals, and humans from harmful microorganisms [41][42][43][44]. Streptomyces species are the main microbes used for the biocontrol of plant diseases because of their wide host range.…”

Section: Discussionmentioning

confidence: 99%

Screening, Identification, and Fermentation of a Biocontrol Strain against Peony Southern Blight and Extraction of Secondary Metabolites

Song,

Wang,

Sun

et al. 2024

Agriculture

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A bacterial strain (WM-37) was isolated from soil and identified as Streptomyces rectiviolaceus on the basis of morphological, physiological, biochemical, and 16S rRNA characteristics. The strain was screened regarding its potential use for controlling the pathogen causing peony southern blight. To enhance the secondary metabolite yield, submerged fermentation was conducted according to a single-factor trial and response surface method. Metabolite production peaked under the following conditions: 250.00 mL flask containing 100.00 mL culture medium consisting of 20.00 g·L−1 soluble starch, 3.86 g·L−1 ammonium sulfate, 0.50 g·L−1 sodium chloride, 0.50 g·L−1 dipotassium phosphate, 0.50 g·L−1 magnesium sulfate, and 0.01 g·L−1 ferrous sulfate; inoculation amount, 7.74%; temperature, 30 °C; initial pH, 7.00; incubation time, 7 days; and rotational speed, 160 rpm. The fermentation broth was absorbed by D101 macroporous resin and eluted with an ethanol-water gradient, after which the eluate fractions with antifungal compounds were collected, evaporated, and concentrated to obtain a crude extract. This extract was dissolved in methanol and separated by high-performance liquid chromatography. The active compound was identified as azelaic acid (C9H16O4) on the basis of ultra-high-resolution mass spectrometry and analyses of publicly available data. These findings suggest that Streptomyces rectiviolaceus WM-37 may be a viable biocontrol agent effective against the pathogen responsible for peony southern blight.

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Section: Discussionmentioning

confidence: 99%

Screening, Identification, and Fermentation of a Biocontrol Strain against Peony Southern Blight and Extraction of Secondary Metabolites

Song,

Wang,

Sun

et al. 2024

Agriculture

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“…Similarly, Streptomyces sp. PBR36 contains smBGCs that encode for ulleungmycin [ 99 ], griseoviridin [ 100 ], tylactone [ 101 ], and istamycin [ 102 ], all of which have antimicrobial activity against MDR bacterial pathogens. PBR36 also encodes the biosynthesis of abyssomicin C and actinomycin D gene clusters.…”

Section: Discussionmentioning

confidence: 99%

Potentiality of Actinomycetia Prevalent in Selected Forest Ecosystems in Assam, India to Combat Multi-Drug-Resistant Microbial Pathogens

2023

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Actinomycetia are known for their ability to produce a wide range of bioactive secondary metabolites having significant therapeutic importance. This study aimed to explore the potential of actinomycetia as a source of bioactive compounds with antimicrobial properties against multi-drug-resistant (MDR) clinical pathogens. A total of 65 actinomycetia were isolated from two unexplored forest ecosystems, namely the Pobitora Wildlife Sanctuary (PWS) and the Deepor Beel Wildlife Sanctuary (DBWS), located in the Indo-Burma mega-biodiversity hotspots of northeast India, out of which 19 isolates exhibited significant antimicrobial activity. 16S rRNA gene sequencing was used for the identification and phylogenetic analysis of the 19 potent actinomycetia isolates. The results reveal that the most dominant genus among the isolates was Streptomyces (84.21%), followed by rare actinomycetia genera such as Nocardia, Actinomadura, and Nonomuraea. Furthermore, seventeen of the isolates tested positive for at least one antibiotic biosynthetic gene, specifically type II polyketide synthase (PKS-II) and nonribosomal peptide synthetases (NRPSs). These genes are associated with the production of bioactive compounds with antimicrobial properties. Among the isolated strains, three actinomycetia strains, namely Streptomyces sp. PBR1, Streptomyces sp. PBR36, and Streptomyces sp. DBR11, demonstrated the most potent antimicrobial activity against seven test pathogens. This was determined through in vitro antimicrobial bioassays and the minimum inhibitory concentration (MIC) values of ethyl acetate extracts. Gas chromatography–mass spectrometry (GS-MS) and whole-genome sequencing (WGS) of the three strains revealed a diverse group of bioactive compounds and secondary metabolite biosynthetic gene clusters (smBGCs), respectively, indicating their high therapeutic potential. These findings highlight the potential of these microorganisms to serve as a valuable resource for the discovery and development of novel antibiotics and other therapeutics with high therapeutic potential.

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“…The strains of Streptomyces genus employ their secondary metabolites as weapons to inhibit phytopathogenic fungi ( Amin et al, 2021 ; Hotta, 2021 ; Mahasneh et al, 2021 ; Terra et al, 2021 ). In this study, genome sequencing revealed that the chromosome of the St. albidoflavus St-220 contained 21 conserved biosynthesis gene clusters (BGCs), of which 10 showed high similarities in structure with known BGCs encoding terpenes, non-ribosomal peptides, polyketides, siderophores, and ectoines, which had been proven to participate in the regulation of antimicrobial activities of Streptomyces strains ( van Bergeijk et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

Wang

Jiang³

et al. 2022

Front. Plant Sci.

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Root rot disease caused by Fusarium oxysporum is a devastating disease of Salvia miltiorrhiza and dramatically affected the production and quality of Sa. miltiorrhiza. Besides the agricultural and chemical control, biocontrol agents can be utilized as an additional solution. In the present study, an actinomycete that highly inhibited F. oxysporum was isolated from rhizosphere soil and identified as based on morphological and molecular characteristics. Greenhouse assay proved that the strain had significant biological control effect against Sa. miltiorrhiza root rot disease and growth-promoting properties on Sa. miltiorrhiza seedlings. To elucidate the biocontrol and plant growth-promoting properties of St-220, we employed an analysis combining genome mining and metabolites detection. Our analyses based on genome sequence and bioassays revealed that the inhibitory activity of St-220 against F. oxysporum was associated with the production of enzymes targeting fungal cell wall and metabolites with antifungal activities. Strain St-220 possesses phosphate solubilization activity, nitrogen fixation activity, siderophore and indole-3-acetic acid production activity in vitro, which may promote the growth of Sa. miltiorrhiza seedlings. These results suggest that St. albidoflavus St-220 is a promising biocontrol agent and also a biofertilizer that could be used in the production of Sa. miltiorrhiza.

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Basic and applied research on multiple aminoglycoside antibiotic resistance of actinomycetes: an old-timer's recollection

Cited by 5 publications

References 41 publications

Screening, Identification, and Fermentation of a Biocontrol Strain against Peony Southern Blight and Extraction of Secondary Metabolites

Screening, Identification, and Fermentation of a Biocontrol Strain against Peony Southern Blight and Extraction of Secondary Metabolites

Potentiality of Actinomycetia Prevalent in Selected Forest Ecosystems in Assam, India to Combat Multi-Drug-Resistant Microbial Pathogens

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

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