Basic Biochemical Parameters of One Hundred Cataractous Lenses from Egyptian Patients

Abstract

Cataractous lenses from an extended population of 100 Egyptian patients whose age, sex and case history had been recorded, were collected and classified morphologically. After determination of the lens wet weights, lenses were microsectioned and basic parameters such as wet weight, dry weight, water content and contents of water-soluble and water-insoluble crystallins of the samples were determined. The data obtained document moderate as well as drastic changes of the investigated parameters along with aging and cataractogenesis, and extend our knowledge from small numbers of hitherto investigated human lenses to a considerably high number of cases.

Distribution of Water-Soluble Crystallins in Microsectioned Cataractous Lenses from One Hundred Egyptian Patients

“…Vlicrosectioning was performed as described previ ously [7][8][9][10]. Preparation of crystallin samples was de scribed in detail by Bours et al [11]. Thin-layer isoe lectric focusing (IEF) was performed according to Bours et al [12].…”

“…All types of cataractous lenses show, cou pled to high levels of WI crystallins [11], a pro gressed loss of |3S-and pL-crystallins in the or der: NS, PSC, CC, CNC, NC ( fig. 2-4a) com pared to the normal lens ( fig.…”