This article presents information on the effect of seedling thickness and weeding on cotton weight per bag and cotton yield. The research was conducted in cotton varieties S-6524, S-6560, UzPITI-202 in the conditions of typical gray soils of Tashkent region, and in each selected variety 80-90; 100-110 and 120-130 thousand bushes/ha were planted. The thickness of each seedling is 11-12 in the background; 13-14; On 15-16 harvest branches, manual pruning and no pruning were placed. By the end of the growing season, the cotton yield and cotton weight per boll of each option were determined. The highest results were obtained from the options where the cotton variety S-6560 was planted at 80-90 and 100-110 thousand bushels/ha, at 13-14 harvest branches, according to the seedling thickness. According to this, the weight of cotton in one sack is 0.57-0.41 g compared to unspun cotton. ha, 1st harvest weight increased by 6.5-3.1%, yield increased by 3.7-2.6 tons/ha. Also, when the UzPITI-202 cotton variety was maintained at different seedling thicknesses and weeding was carried out in it, the highest values were obtained from the variant where cotton was maintained at a seedling thickness of 80-90 and 100-110 thousand bushels/ha, weeding was carried out at 13-14 harvest branches, and cotton per boll it was noted that the weight of the 1st harvest increased by 4.5%, the yield increased by 3.5-2.7 t/h compared to the non-chilled one. However, it was observed that the seedling thickness in non-chilled options is lower in the background with 80-90 thousand leaves than in the background with 100-110 thousand bush/ha.