Basic fibroblast growth factor corrects proliferative derangement of bone marrow stroma and CD34+ population following allogeneic stem cell transplantation

Dynamic of Distribution of Human Bone Marrow-Derived Mesenchymal Stem Cells After Transplantation into Adult Unconditioned Mice

“…MSC transplantation has been envisioned as a proper therapy for attenuating or correcting alterations in the hematopoietic microenvironment (24) or for promoting engraftment and differentiation of hematopoietic stem cells in the bone marrow (3, 25, 26). There- (3) Bone (4) Skeletal muscle (4) Skeletal muscle (5) Tail (3) Tail (6) Tooth (2) Tooth (2) Cardiac muscle (9), ear (4), liver (7) a Summary of mouse tissues where the human-␤-globin gene signal was detected (15 min-13 mo) after injection of human MSC.…”

Age-Related Changes in the Osteogenic Differentiation Potential of Mouse Bone Marrow Stromal Cells

“…(21) Evidence also showed that the bone marrow cells are capable of forming CFU-Fs without feeder cells. (26,(32)(33)(34) To clarify whether feeder cells are necessary, we performed CFU-F assays using lethally irradiated guinea pig bone marrow cells as described by Kuznetsov and Gehron (21) or simply using regular growth media supplemented with 20% FBS, because both the murine CFU-F Assay Kit and feeder cell methods are supplemented with 20% serum in the media. Results…”

Reduced supportive capacity of bone marrow stroma upon chemotherapy is mediated via changes in glycosaminoglycan profile

“…There is increasing evidence that stromal proteoglycans (PGs), consisting of a core protein and glycosaminoglycan (GAG) chains, play a critical role in supporting hematopoiesis. (Gupta et al, 1998) High dose chemotherapy and radiation, as applied in stem cell transplantations, have been found to impair the hematopoiesis-supportive capacity of bone marrow stroma (Galotto et al, 1999;O'Flaherty et al, 1995;Novitzky et al, 2001). Although a decrease in stromal progenitors has been implicated (Galotto et al, 1999), such a decrease does not unequivocally correlate with the in vitro capacity of stroma to support hematopoiesis (Carlo-Stella et al, 1997;Novitzky et al, 2001).…”