Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway

Narong, Siriluck; Leelawat, Kawin

doi:10.3892/ol.2011.333

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…bFGF also has extensive biological activities, which is present in basement membranes, in the subendothelial extracellular matrix of blood vessels in normal tissue and in periodontal ligament ( 24 ). The study showed that bFGF can regulate cell proliferation and differentiation ( 25 , 26 ) and is able to promote angiogenesis ( 27 ) and nerve regeneration ( 28 , 29 ). In an in vitro experiment, bFGF was found to sustain self-renewal ability, while maintaining differentiation potency ( 30 , 31 ).…”

Section: Introductionmentioning

confidence: 99%

The differential effect of basic fibroblast growth factor and stromal cell‑derived factor‑1 pretreatment on bone morrow mesenchymal stem cells osteogenic differentiation potency

Wang

Liu

et al. 2017

Mol Med Report

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In situ tissue engineering has become a novel strategy to repair periodontal/bone tissue defects. The choice of cytokines that promote the recruitment and proliferation, and potentiate and maintain the osteogenic differentiation ability of mesenchymal stem cells (MSCs) is the key point in this technique. Stromal cell-derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) have the ability to promote the recruitment, and proliferation of MSCs; however, the differential effect of SDF-1 and bFGF pretreatment on MSC osteogenic differentiation potency remains to be explored. The present study comparatively observed osteogenic differentiation of bone morrow MSCs (BMMSCs) pretreated by bFGF or SDF-1 in vitro. The gene and protein expression levels of alkaline phosphatase (ALP), runt related transcription factor 2 (Runx-2) and bone sialoprotein (BSP) were detected using reverse transcription-quantitative polymerase chain reaction and western blotting. The results showed that the expression of ALP mRNA on day 3, and BSP and Runx-2 mRNA on day 7 in the bFGF pretreatment group was significantly higher than those in SDF-1 pretreatment group. Expression levels of Runx-2 mRNA, and ALP and Runx-2 protein on day 3 in the SDF-1 pretreatment group were higher than those in the bFGF pretreatment group. However, there was no significant difference in osteogenic differentiation ability on day 14 and 28 between the bFGF- or SDF-1-pretreatment groups and the control. In conclusion, bFGF and SDF-1 pretreatment inhibits osteogenic differentiation of BMMSCs at the early stage, promotes it in the medium phase, and maintains it in the later stage during osteogenic induction, particularly at the mRNA level. Out of the two cytokines, bFGF appeared to have a greater effect on osteogenic differentiation.

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Section: Introductionmentioning

confidence: 99%

The differential effect of basic fibroblast growth factor and stromal cell‑derived factor‑1 pretreatment on bone morrow mesenchymal stem cells osteogenic differentiation potency

Wang

Liu

et al. 2017

Mol Med Report

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“…CNV-genes CACNA1 and CACNG7, two calcium channel subunits, compound with RAS guanyl nucleotide releasing proteins which are guanyl nucleotide exchange factors that activate Ras and related GTPases such as RAP [46] . CNV-genes FGFR2 could induce cholangiocarcinoma cell migration via activation of the MEK1/2 pathway [47] . CNV-TF PSG1 regulated upstream activator PTPN7, downstream target ATF2 [48] of MAPK pathway.…”

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of Transcriptional Regulatory Network and Copy Number Variation in Intrahepatic Cholangiocarcinoma

Lian

et al. 2014

PLoS ONE

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BackgroundTranscriptional regulatory network (TRN) is used to study conditional regulatory relationships between transcriptional factors and genes. However few studies have tried to integrate genomic variation information such as copy number variation (CNV) with TRN to find causal disturbances in a network. Intrahepatic cholangiocarcinoma (ICC) is the second most common hepatic carcinoma with high malignancy and poor prognosis. Research about ICC is relatively limited comparing to hepatocellular carcinoma, and there are no approved gene therapeutic targets yet.MethodWe first constructed TRN of ICC (ICC-TRN) using forward-and-reverse combined engineering method, and then integrated copy number variation information with ICC-TRN to select CNV-related modules and constructed CNV-ICC-TRN. We also integrated CNV-ICC-TRN with KEGG signaling pathways to investigate how CNV genes disturb signaling pathways. At last, unsupervised clustering method was applied to classify samples into distinct classes.ResultWe obtained CNV-ICC-TRN containing 33 modules which were enriched in ICC-related signaling pathways. Integrated analysis of the regulatory network and signaling pathways illustrated that CNV might interrupt signaling through locating on either genomic sites of nodes or regulators of nodes in a signaling pathway. In the end, expression profiles of nodes in CNV-ICC-TRN were used to cluster the ICC patients into two robust groups with distinct biological function features.ConclusionOur work represents a primary effort to construct TRN in ICC, also a primary effort to try to identify key transcriptional modules based on their involvement of genetic variations shown by gene copy number variations (CNV). This kind of approach may bring the traditional studies of TRN based only on expression data one step further to genetic disturbance. Such kind of approach can easily be extended to other disease samples with appropriate data.

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“…Data have been accumulated, implicating multiple factors and mechnisms in these processes. Narong and Leelawat 7 observed that by activation of the MEK1/2 pathway, basic fibroblast growth factor induced cholangiocarcinoma cell migration. Overexpression of extracellular matrix protein-1 (ECM1), nuclear protein-1 (NUPR1) and arsenate resistance protein 2 was associated with proliferation and motility of cholangiocarcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

In vitro neuraotropic growth of cholangiocarcinoma: an experimental study

et al. 2013

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SummaryObjectivePerineural invasion of cholangiocarcinoma happens in the early stage of the disease but is often not recognized until its later stages. Research about the behaviour and mechanism of perineural invasion by cholangiocarcinoma is urgently needed for a useful new model. The aim of this work is to establish a novel model to address the problem.DesignNeural cells and cholangiocarcinoma cells were co-cultured to mimic the neurotropic invasion of cholangiocarcinoma.SettingHuman embryonic stem cells were induced to form neural cells by glial cell-derived neurotropic factor and retinoic acid; neural cells and cholangiocarcinoma cells were co-cultured in Transwell chamber.ParticipantsHuman embryonic stem cells and cholangiocarcinoma cells were applied.Main outcome measuresPaired t-test was used to compare the counts of penetrating cholangiocarcinoma cells in co-culture and control group.ResultsFormation of neurospheres and neural-like cells were observed following induction at 24 and 48 h, respectively; synapses were viewed to protrude from neural-like cell bodies after incubation for 96 h. Forty-eight hours after incubation, immunocytochemical staining of the cells showed that synaptophysin and glial fibrillary acidic protein were expressed in the neuron-like cells and gliocytes-like cells, respectively. The cholangiocarcinoma cells that had penetrated through the Matrigel/polyethylene terephthalate membrane from the upper chamber to the lower chamber of the Transwell in the co-culture group were significantly more numerous than those in the control group (68 ± 8.3/field versus 46 ± 5.7/field, P < 0.05).ConclusionThe novel model is a valuable tool to study the perineural invasion of cholangiocarcinoma.

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Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway

Cited by 9 publications

References 20 publications

The differential effect of basic fibroblast growth factor and stromal cell‑derived factor‑1 pretreatment on bone morrow mesenchymal stem cells osteogenic differentiation potency

The differential effect of basic fibroblast growth factor and stromal cell‑derived factor‑1 pretreatment on bone morrow mesenchymal stem cells osteogenic differentiation potency

Integrative Analysis of Transcriptional Regulatory Network and Copy Number Variation in Intrahepatic Cholangiocarcinoma

In vitro neuraotropic growth of cholangiocarcinoma: an experimental study

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