Basic Guide to Detection and Monitoring of Potentially Toxic Cyanobacteria

Salmaso, Nico; Bernard, Cécile; Humbert, Jean‐François; Akçaalan, Reyhan; Albay, Meriç; Ballot, Andreas; Catherine, Arnaud; Fastner, Jutta; Häggqvist, Kerstin; Horecká, Mária; Izydorczyk, Katarzyna; Latife, Köker; Komárek, Jiří; Maloufi, Selma; Mankiewicz-Boczek, J.; Metcalf, James S.; Quesada, Antonio; Quiblier, Catherine; Yéprémian, Claude

doi:10.1002/9781119068761.ch6

Cited by 21 publications

(27 citation statements)

References 57 publications

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“…According to the literature data [25,32], 36 species of cyanobacteria in Lake Peipsi are potentially toxic. A Mantel permutation test showed a significant relationship between the biomass of potentially toxic species and mcyE gene abundances (r = 0.43, p < 0.01, 999 permutations, n = 141).…”

Section: Abundance Of Mcye Genesmentioning

confidence: 99%

“…A high temperature stimulates cyanobacterial growth, with many species reaching their maximum growth rate above 25 • C [11,23]. As the nutrient dynamics in lakes is strongly affected by the external loading of phosphorus (P) and nitrogen (N) [24], the abundance and community composition of cyanobacteria are mainly linked to these two environmental factors [25]. Traditionally, high P concentration is considered as the main risk factor for cyanobacterial blooms [26].…”

Section: Introductionmentioning

confidence: 99%

“…To evaluate the risks to public health, data on potentially toxic cyanobacteria and cyanotoxins need to be provided as early as possible [11]. DNA based methods (e.g., polymerase chain reaction-PCR and quantitative PCR) comprise a valuable toolbox for both the detection and quantification of potentially toxic cyanobacteria [25,[36][37][38]. However, the relationship between the cellular microcystin (MC) concentration and the copy number of mcy genes or MC producer's biomass is not straightforward.…”

Section: Introductionmentioning

confidence: 99%

“…As the toxin quota per cell directly reflects the safety of the waterbody, it is important to clarify how the copy number of mcy genes, MC concentration and environmental factors are related to this specific parameter. Quantitative PCR (qPCR) is a time-and cost-effective tool to evaluate the proportion of potential toxin-producing genotypes over the cyanobacterial population, as well as to investigate how environmental parameters determine toxicity potential at the spatio-temporal scale [25,34]. Consequently, qPCR can help us understand the mechanisms that trigger toxic blooms and can be used as an early warning tool for lake managers.…”

Section: Introductionmentioning

confidence: 99%

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Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi

Panksep

Tamm

Mantzouki

et al. 2020

Toxins

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Global warming, paired with eutrophication processes, is shifting phytoplankton communities towards the dominance of bloom-forming and potentially toxic cyanobacteria. The ecosystems of shallow lakes are especially vulnerable to these changes. Traditional monitoring via microscopy is not able to quantify the dynamics of toxin-producing cyanobacteria on a proper spatio-temporal scale. Molecular tools are highly sensitive and can be useful as an early warning tool for lake managers. We quantified the potential microcystin (MC) producers in Lake Peipsi using microscopy and quantitative polymerase chain reaction (qPCR) and analysed the relationship between the abundance of the mcyE genes, MC concentration, MC variants and toxin quota per mcyE gene. We also linked environmental factors to the cyanobacteria community composition. In Lake Peipsi, we found rather moderate MC concentrations, but microcystins and microcystin-producing cyanobacteria were widespread across the lake. Nitrate (NO3−) was a main driver behind the cyanobacterial community at the beginning of the growing season, while in late summer it was primarily associated with the soluble reactive phosphorus (SRP) concentration. A positive relationship was found between the MC quota per mcyE gene and water temperature. The most abundant variant—MC-RR—was associated with MC quota per mcyE gene, while other MC variants did not show any significant impact.

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Section: Abundance Of Mcye Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi

Panksep

Tamm

Mantzouki

et al. 2020

Toxins

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“…They are also well known for their production of a wide variety of natural bioactive products, including some potent toxins (e.g., microcystins, anatoxins, saxitoxins) [2,3]. Due to the remarkable capability of cyanobacteria to proliferate and form toxic blooms that induce potential human health consequences [4], numerous studies have been conducted to develop tools for the monitoring of cyanobacterial blooms [5,6] or effective strategies for the mitigation of their overgrowth [7]. On the contrary, cyanotoxins could also constitute a promising opportunity for drug development, notably for certain cancer therapies [8].…”

Section: Introductionmentioning

confidence: 99%

Natural Products from Cyanobacteria: Focus on Beneficial Activities

Demay

Bernard²,

Reinhardt

et al. 2019

Preprint

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Cyanobacteria are photosynthetic microorganisms that colonize diverse environments worldwide, ranging from ocean to freshwaters, soils, and extreme environments. Their adaptation capacities and the diversity of natural products (molecules, metabolites, or compounds) that they synthesize support the cyanobacterial success for the colonization of their respective ecological niches. Although cyanobacteria are well-known for their toxin production and their relative deleterious consequences, they also produce a large variety of molecules that exhibit beneficial properties with high potential for various fields of application (e.g., synthetic analog of the dolastatin 10 used against Hodgkin lymphoma). The present review specially focuses on the beneficial activities of cyanobacterial molecules described so far. Based on an analysis of 670 papers, it appears that more than 90 genera of cyanobacteria have been found to produce compounds with potential beneficial activities, most of them belonging to the orders Oscillatoriales, Nostocales Chroococcales, and Synechococcales. The rest of the cyanobacterial orders (i.e., Pleurocapsales, Chroococcidiopsales, and Gloeobacterales) remain poorly explored in terms of their molecular diversity and relative bioactivity. The diverse cyanobacterial molecules presenting beneficial bioactivities belong to 10 different chemical classes (alkaloids, depsipeptides, lipopeptides, macrolides/lactones, peptides, terpenes, polysaccharides, lipids, polyketides, and others) that exhibit 14 major kinds of bioactivity. However, no direct relation between the chemical class and the bioactivity of these molecules has been demonstrated. We further selected and specifically described 50 molecule families according to their specific bioactivities and their potential uses in pharmacology, cosmetology, agriculture, or other specific fields of interest. This up-to-date review takes advantage of the recent progresses in genome sequencing and biosynthetic pathway elucidation, and presents new perspectives for the rational discovery of new cyanobacterial metabolites with beneficial bioactivity.

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