Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo, Zhi-Fang; Lee, Hyo-Yeon; Kang, Hong‐Gyu

doi:10.3390/ijms24021419

Cited by 25 publications

(8 citation statements)

References 159 publications

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“…Basic helix-loop-helix (bHLH) TFs play a crucial roles in a variety of processes of plant growth, metabolism, and abiotic stress response ( Zuo et al., 2023 ). However, the characterization of bHLH genes in P. sibirica has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

Liu,

Wen,

Wang

et al. 2023

Front. Plant Sci.

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The basic helix-loop-helix (bHLH) family is one of the most well-known transcription factor families in plants, and it regulates growth, development, and abiotic stress responses. However, systematic analyses of the bHLH gene family in Prunus sibirica have not been reported to date. In this study, 104 PsbHLHs were identified and classified into 23 subfamilies that were unevenly distributed on eight chromosomes. Nineteen pairs of segmental replication genes and ten pairs of tandem replication genes were identified, and all duplicated gene pairs were under purifying selection. PsbHLHs of the same subfamily usually share similar motif compositions and exon-intron structures. PsbHLHs contain multiple stress-responsive elements. PsbHLHs exhibit functional diversity by interacting and coordinating with other members. Twenty PsbHLHs showed varying degrees of expression. Eleven genes up-regulated and nine genes down-regulated in −4°C. The majority of PsbHLHs were highly expressed in the roots and pistils. Transient transfection experiments demonstrated that transgenic plants with overexpressed PsbHLH42 have better cold tolerance. In conclusion, the results of this study have significant implications for future research on the involvement of bHLH genes in the development and stress responses of Prunus sibirica.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

Liu,

Wen,

Wang

et al. 2023

Front. Plant Sci.

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“…A standard bHLH domain is usually composed of two parts, including a basic region positioning at the N-terminus of bHLH domain, which is known to recognize and bind to the E-box consensus (CANNTG), followed by a HLH region positioning at the C-terminus of the bHLH domain that is accountable for homo- and heterodimer formation [ 14 ]. The bHLH TFs engage in a multitude of physiological events, from plant growth and development (e.g., embryo growth, anthers development, fruit dehiscence, and seed dispersal) to stress adaptation (e.g., pathogen infection, cold stress, and salt stress) [ 15 ]. Currently, some bHLH proteins that are involved in fruit ripening regulation have been characterized.…”

Section: Introductionmentioning

confidence: 99%

Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111

Wu,

Cai,

et al. 2024

Horticulture Research

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Texture softening is a physiological indicator of fruit ripening, which eventually contributes to fruit quality and consumer’s acceptance. Despite great progress has been made in identification of the genes related to fruit softening, the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated. Here, a novel bHLH gene, designated as MabHLH28, was identified because of its significant upregulation in banana fruit ripening. DAP-Seq analysis revealed that MabHLH28 bound to the core sequence of ‘CAYGTG’ presented in promoter regions of fruit softening-associated genes, such as the genes related to cell wall modification (MaPG3, MaPE1, MaPL5, MaPL8, MaEXP1, MaEXP2, MaEXPA2 and MaEXPA15) and starch degradation (MaGWD1 and MaLSF2), and these bindings were validated by EMSA and DLR assays. Transient overexpression and knockdown of MabHLH28 in banana fruit resulted in up- and down-regulation of softening-related genes, thereby hastening and postponing fruit ripening. Furthermore, overexpression of MabHLH28 in tomato accelerated the ripening process by elevating the accumulation of softening-associated genes. In addition, MabHLH28 showed interaction withMaWRKY49/111 and itself to form protein complexes, which could combinatorically strengthen the transcription of softening-associated genes. Taken together, our findings suggest that MabHLH28 mediates fruit softening by upregulating the expression of softening-related genes either alone or in combination with MaWRKY49/111.

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“…A number of evidence has shown that bHLH family genes are involved in plant development processes [21,22]. For example, the bHLH TF SPATULA (SPT) inhibited root growth and size through limiting cell proliferation and expansion in Arabidopsis [23].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression of CmobHLH Genes in Pumpkin

Luo

Wang

et al. 2023

Horticulturae

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The transcription factor bHLH gene family plays fundamental roles in plant development and mitigating diverse biotic and abiotic stresses. However, the information of bHLH genes in pumpkin (Cucurbita moschata) is still unknown. In this current study, 222 CmobHLH genes were identified and mapped onto different chromosomes through bioinformatics analysis in pumpkin. CmobHLH and AtbHLH proteins could be classified into 19 subfamilies according to the phylogenetic tree. CmobHLH proteins within the same subfamily had similar motif composition and gene structures. Gene ontology (GO), cis-regulatory elements (CREs) and protein–protein interaction analyses suggested the potential regulatory roles of CmobHLH genes during the plant development process and abiotic stresses response in pumpkin. Tissue expression patterns based on transcriptome data demonstrated that CmobHLH genes were involved in pumpkin development process, and they had unique functions in different tissues. The expression patterns of five selected CmobHLH genes after exposure to abiotic stresses showed that the CmobHLH genes played varied roles in the stress responses of pumpkin to NaCl, waterlogging, cold, ABA and drought. In brief, these findings offer important information for further functional research of CmobHLH genes and resistance breeding in pumpkin.

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Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Cited by 25 publications

References 159 publications

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family in Prunus sibirica

Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111

Molecular Characterization and Expression of CmobHLH Genes in Pumpkin

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