Basic Mechanisms in RNA Polymerase I Transcription of the Ribosomal RNA Genes

Goodfellow, Sarah J.; Zomerdijk, Joost C.B.M.

doi:10.1007/978-94-007-4525-4_10

Cited by 164 publications

(152 citation statements)

References 187 publications

(275 reference statements)

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“…Together, tagetitoxin (18 M) and ␣-amanitin (4 ng/l) were able to completely abolish transcription, indicating that Pol II and Pol III, but not RNA polymerase I, initiate transcription from Pol II promoters in vitro (Fig. 3C), which reflect prior observations that SL1 binding is solely mediated by its TAFs (9).…”

Section: Multiple Polymerases Can Utilize Rna Pol II Promoters Insupporting

confidence: 78%

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RNA Polymerase III Accurately Initiates Transcription from RNA Polymerase II Promoters in Vitro

Duttke

2014

Journal of Biological Chemistry

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Background: RNA polymerase II (Pol II) and III (Pol III) transcription systems are reported to regulate non-overlapping sets of genes. Results: Pol III can initiate transcription from Pol II promoters with AT-rich sequence. The DNA template to nuclear extract ratio determines the predominant polymerase. Conclusion: Pol II and Pol III transcription initiation can overlap. Significance: RNA polymerase specificity is variable and depends on the transcription conditions.

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Section: Multiple Polymerases Can Utilize Rna Pol II Promoters Insupporting

confidence: 78%

“…Likewise, the transcription initiation factors for Pol I and Pol III (SL1/Tif-1B and TFIIIB, respectively) contain a TFIIB-related protein and TBP (or a TBP-related protein (Trf1) in Drosophila) counterparts (7)(8)(9)20).…”

mentioning

confidence: 99%

RNA Polymerase III Accurately Initiates Transcription from RNA Polymerase II Promoters in Vitro

Duttke

2014

Journal of Biological Chemistry

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“…Phosphorylation of Rrn3 by kinases linked to nutrient or energy availability, mitogen activation, stress and cell cycle cues modulate its transcriptional initiation activity. These observations led to the proposal that transcription initiation via modulation of Rrn3 activity is the major rate-limiting step for rRNA synthesis in mammalian cells [22,[26][27][28][29][30][31]. This model is supported by a study that utilized live cell imaging combined with computational modeling to analyze the transcription complex dynamics of rDNA transcription regulation during the cell cycle.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Cell cycle and growth stimuli regulate different steps of RNA polymerase I transcription

Hung

Lesmana

Peck

et al. 2017

Gene

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, Cell cycle and growth stimuli regulate different steps of RNA polymerase I transcription. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Gene(2016Gene( ), doi: 10.1016Gene( /j.gene.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T A C C E P T E D M A N U S C R I P T 3 AbstractTranscription of the ribosomal RNA genes (rDNA) by RNA Polymerase I (Pol I) is a major control step for ribosome synthesis and is tightly linked to cellular growth.However, the question of whether this process is modulated primarily at the level of transcription initiation or elongation is controversial. Studies in markedly different cell types have identified either initiation or elongation as the major control point. In this study, we have re-examined this question in NIH3T3 fibroblasts using a combination of metabolic labelling of the 47S rRNA, chromatin immunoprecipitation analysis of Pol I and overexpression of the transcription initiation factor Rrn3. Acute manipulation of growth factor levels altered rRNA synthesis rates over 8-fold without changing Pol I loading onto the rDNA. In fact, robust changes in Pol I loading were only observed under conditions where inhibition of rDNA transcription was associated with chronic serum starvation or cell cycle arrest. Overexpression of the transcription initiation factor Rrn3 increased loading of Pol I on the rDNA but failed to enhance rRNA synthesis in either serum starved, serum treated or G0/G1 arrested cells. Together these data suggest that transcription elongation is rate limiting for rRNA synthesis. We propose that transcription initiation is required for rDNA transcription in response to cell cycle cues, whereas elongation controls the dynamic range of rRNA synthesis output in response to acute growth factor modulation.

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“…Initiation of rDNA transcription in yeast requires the sequential assembly of factors on different promoter elements (reviewed in [6,7]). The upstream activating factor (UAF) binds the UE [2], while the core factor (CF) binds the CE [8].…”

Section: Introductionmentioning

confidence: 99%