Basic peroxidases in isolated vacuoles of nicotiana tabacum L.

Schloss, Patrick D.; Walter, C.A.; Mäder, Michael

doi:10.1007/bf00397892

Cited by 67 publications

(24 citation statements)

References 19 publications

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“…The pattern of peroxidase isoforms from the ionically bound cell wall fraction (12,13) differs from the pattern for the CWFS as obtained here and elsewhere (3). This difference may be explained by evidence that the technique used to obtain ionically bound cell wall peroxidases is subject to cytoplasmic contamination (20).…”

Section: Discussioncontrasting

confidence: 42%

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Shinkle

Swoap²,

Simon³

et al. 1992

Plant Physiol.

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Solutions were obtained from the cell wall free space of red light-grown cucumber (Cucumis sativus L.) hypocotyl sections by a low-speed centrifugation technique. The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution. Peroxidase activity centrifuged from the cell wall of red light-grown cucumber hypocotyl section could be resolved into at least three cathodic isoforms and two anodic isoforms by isoelectric focusing. Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme. The increase in peroxidase activity was detectable within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiation. The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.Peroxidase, extracted from cell walls by various methods, has been shown to vary in activity with hormone-stimulated growth and naturally occurring gradients of growth rate (3,12,13,18,19,23). An inverse relationship between growth rate and cell wall peroxidase activity has been found. One model for peroxidase action holds that cell wall extensibility can be regulated by the formation of intermolecular crosslinks between wall polysaccharides or proteins via phenolic components such as ferulic acid or tyrosine residues (8, 17). Cross-linking structural wall polymers could alter cell wall extensibility (6,8,10), and intermolecular tyrosine cross-links (7) could affect the function of cell wall proteins as well as their structure.Because extracting peroxidase from the CWFS4 to obtain rapidly changing pools of enzymes has yielded ambiguous results. CWFS peroxidases have been obtained from several tissues exhibiting changes in growth rate, by treatment of intact stem segments with vacuum infiltration followed by low-speed centrifugation (3,14,15,18,23), but no consistent pattern has been found. In pea stems showing an auxin-induced increase in elongation, a decrease in CWFS peroxidase activity was observed (18). However, in corn coleoptiles in which red light increases elongation rate, the peroxidase activity extracted from the CWFS also increased instead of decreasing as expected (14). Hypocotyls of light-grown Sinapis show decreasing activities in specific isozymes in tissues showing increased growth rates, but the enzyme activity changes were slower than the changes in growth rate (3).We have utilized the rapid inhibition of stem elongation in Cucumis seedlings as a system to further test the hypothesis that changes in cell wall peroxidase activity mediate changes in stem elongation rate. In Cucumis hypocotyls,...

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Section: Discussioncontrasting

confidence: 42%

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Shinkle

Swoap²,

Simon³

et al. 1992

Plant Physiol.

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“…Peroxidase is ir-ivolved in n-iany different processes in plant cell metabolism but is mostly localized in the vacuole and in the cell wall (Schloss, Walter & Mader, 1987). Fry (1980) demonstrated that 'peroxidase stiffens the cell wall by catalysis of the oxidation of the cell-wall phenolic compounds to form the more hydrophobic biphenyls, polymers or quinones, any of which could protect the wall polysaccharides against attack by enzymes or water'.…”

Section: Introductionmentioning

confidence: 99%

Cell‐wall‐bound peroxidase activity in roots of mycorrhizal Allium porrum

1988

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“…This peroxidase uses AA' as an electron donor. Its rapid inactivation in the absence of AA can be used to distinguish it from other peroxidases (2) that are localized in the large central vacuole of plant cells (3,9,15,16) and outside the cells in the apoplast (4,5,10,13,18). The apoplastic peroxidases are involved in the formation of cross-linkings within cell wall constituents.…”

mentioning

confidence: 99%

Effects of the Air Pollutant SO₂ on Leaves

Takahama¹,

Veljovic-Iovanovic²,

Heber³

1992

Plant Physiol.

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After S02 has entered leaves of spinach (Spinacia oleracea) through open stomata and been hydrated in the aqueous phase of cell walls, the sulfite formed can be oxidized to sulfate by an apoplastic peroxidase that is normally involved in phenol oxidation. The oxidation of sulfite is competitive with the oxidation of phenolics. During sulfite oxidation, the peroxidase is inhibited. In the absence of ascorbate, which is a normal constituent of the aqueous phase of the apoplast, peroxidative sulfite oxidation facilitates fast additional sulfite oxidation by a radical chain reaction. By scavenging radicals, ascorbate inhibits chain initiation and sulfite oxidation. Even after exposure of leaves to high concentrations of SO2, which inhibited photosynthesis, the redox state of ascorbate remained almost unaltered in the apoplastic space of the leaves. It is concluded that the oxidative detoxification of SO2 in the apoplast outside the cells is slow. Its rate depends on the rate of apoplastic hydrogen peroxide generation and on the steady-state apoplastic concentrations of phenolics and sulfite. The affinity of the peroxidase for phenolics is higher than that for sulfite.

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Basic peroxidases in isolated vacuoles of nicotiana tabacum L.

Cited by 67 publications

References 19 publications

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Cell‐wall‐bound peroxidase activity in roots of mycorrhizal Allium porrum

Effects of the Air Pollutant SO₂ on Leaves

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Basic peroxidases in isolated vacuoles of nicotiana tabacum L.

Cited by 67 publications

References 19 publications

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity

Cell‐wall‐bound peroxidase activity in roots of mycorrhizal Allium porrum

Effects of the Air Pollutant SO2 on Leaves

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Effects of the Air Pollutant SO₂ on Leaves