Basic Principles and Analytical Application of Derivative Spectrophotometry

Karpińska, Joanna

doi:10.5772/37673

Cited by 33 publications

(17 citation statements)

References 29 publications

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“…expressing n as the derivative order, n D xλ or n D x,v as the value of an order derivative of an analyte (x), λ as the analytical wavelength, v as the wavelength number, and A as the absorbance (Karpinska 2012). The second derivative spectra showed fine spectral structures with at least three negative peaks.…”

Section: Assays Of Lipid Peroxidation Markersmentioning

confidence: 99%

A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

Cebe

Yanar

Atukeren

et al. 2014

AGE

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Age-related myocardial dysfunction has important implications with impaired redox homeostasis. Current study focused on investigation of redox homeostasis and histopathological changes in the myocardium of mimetically (MA), naturally aged (NA), and young control (YC) rats. Chronic D-galactose administration to young male Wistar rats (5 months old) was used to set up experimental aging models. We investigated 16 different oxidative damage biomarkers which have evaluated redox homeostasis of cellular macromolecules such as protein, lipid, and DNA. As a protein oxidation biomarker, advanced oxidation end products, protein carbonyl groups, protein-bound advanced glycation end products, dityrosine, kynurenine, and N-formylkynurenine concentrations in MA and NA rats were found to be significantly higher compared to those in YC rats. On the other hand, the levels of protein thiol groups were not significantly different between groups, whereas lipid peroxidation biomarkers such as conjugated diens, lipid hydroperoxides, and malondialdehyde in MA and NA rats were found to be significantly higher in comparison to those in YCs. For the assessment of oxidative DNA damage, we analyzed eight hydroxy-5'-deoxyguanosine concentrations of MA and NA groups which were higher than YCs. As an antioxidant status in the MA and NA groups, Cu-Zn superoxide dismutase, ferric reducing antioxidant power, and total thiol levels were lower than those in the YCs. Only nonprotein thiol levels were not significantly different. We also observed similar histopathological changes in MA and NA rats. We concluded that the mimetic aging model could be considered as a reliable experimental model for myocardial senescence.

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Section: Assays Of Lipid Peroxidation Markersmentioning

confidence: 99%

A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

Cebe

Yanar

Atukeren

et al. 2014

AGE

View full text Add to dashboard Cite

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“…Distorted derivative spectrum is a result of inappropriate polynomial degree. In case of multicomponent analysis, the spectral differences of assayed compounds and their selective determination can be increased by the use of different polynomial degrees [2].…”

Section: Zero Order Derivative Spectrummentioning

confidence: 99%

“…The weakness was conquering in 1970s with microcomputers which gave derivative spectra in more specific, simple, rapid and reproducible way. This made to enlarge applicability of derivative method; Derivatization of spectra augments selectivity by eradicates spectral interferences [2][3].…”

Section: Introductionmentioning

confidence: 99%

A Review on Derivative Uv-Spectrophotometry Analysis of Drugs in Pharmaceutical Formulations and Biological Samples Review

Redasani

Patel

Marathe

et al. 2018

J. Chil. Chem. Soc.

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The review article deals with theoretical aspects of Derivative UV-Spectrophotometry. The method gains significance using the first and second derivative of the transmission spectra with respect to wavelength. Generated optical derivatives are compared to the known numerical derivatives. The derivative spectra from 1 st to 4 th are consequently discussed. This provides valuable insight into the uses and limitations of this technique for chemical analysis. Measurement techniques and methods of obtaining derivative spectra are discussed. The degree of polynomial fit on the smoothness of derivative spectra and signal-to-noise ratio is described. Application of UV derivative spectrometry for determination of single and multicomponent analysis is shown. Derivative spectrophotometry possibly improves the selectivity and sensitivity of determination which has been illustrated.

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“…Metode-metode tersebut membutuhkan biaya yang relatif tinggi dan waktu analisis yang relatif lama. (Karpinska, 2012;Saakov et al, 2013 …”

Section: Pendahuluanunclassified

Penetapan Kadar Hidrokortison Asetat dalam Sediaan Krim Mengandung Pengawet Nipagin secara Spektrofotometri Derivatif Orde Pertama

Hayun¹,

Leswara²,

Zarkasih³

2014

Pharm Sci Res

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AbstrakMetode spektrofotometri yang sederhana dan cepat telah dikembangkan untuk analisis hidrokortison asetat dalam sediaan farmasi krim mengandung nipagin sebagai pengawet. Hidrokortison asetat ditentukan dengan mengukur serapan derivat pertama (rasio amplitudo) pada 257,0 nm (zero crossing nipagin). Kurva kalibrasi menunjukkan linier pada rentang 4,0-40,0 ppm hidrokortison asetat (r= 0,9999). Limit deteksi (LOD) dan limit kuantitasi (LOQ) diperoleh masing-masing 0,9617 ppm dan 3,2050 ppm. Metode ini mempunyai presisi yang baik (keterulangan dan presisi intermediet) (RSD <2,0%), sedangkan rerata data hasil uji perolehan kembali (accuracy) adalah 102,03±0,14% dan 100,23±0,69%, masing-masing untuk hidrokortison asetat krim1% dan 2,5%. Metode ini berhasil diterapkan untuk penetapan kadar hidrokortison asetat tiga dari empat sampel sediaan merk dagang dan hasilnya adalah 102,93±0.22%, 108,48±0,19% dan106,67±0,35%, masing-masing untuk sampel A, B dan D. Hasil analisis sampel C menunjukkan kadar hidrokortison asetat lebih dari 110,0% dari yang tertulis pada labelnya, mengindikasikan adanya bahan tambahan lain selain nipagin dalam basis krimnya yang menginterferensi pengukuran hidrokortison asetat. AbstractThe simple and rapid spectrophotometric methods were developed for analysis of hydrocortisone acetate in cream pharmaceutical formulations containing nipagin as preservative. Concentration of hydrocortisone acetate was determined by measuring the first derivative absorption (ratio amplitudes) at 257.0 nm (zero crossing for nipagin). The calibration graphs were linear over the range of 4.0-40.0 ppm of hydrocortisone acetate (r= 0.9999). The limit of detection (LOD) and the limit of quantitation (LOQ) were found to be 0.9617 ppm and 3.2050 ppm, respectively. This method had good precision (repeatability and intermediate precision) with RSD < 2.0% where as the means of the recovery data (accuracy) were 102.03±0.14% and 100.23±0.69% for hydrocortisone acetate cream 1% and 2.5%, respectively. The proposed method was applied for the determination of hydrocortisone acetate in three out of four commercial cream formulations samples and the results of label claim were 102.93±0.22%, 108.48±0.19% and 106.67±0.35% for sample A, B and D, respectively. The result of brand C analysis showed to contain more than 110.0% of the labeled amount of hydrocortisone acetate, indicated there was additive other than nipagin in the cream basis to interfer with the hydrocortisone acetate measurements.

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Basic Principles and Analytical Application of Derivative Spectrophotometry

Cited by 33 publications

References 29 publications

A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

A comprehensive study of myocardial redox homeostasis in naturally and mimetically aged rats

A Review on Derivative Uv-Spectrophotometry Analysis of Drugs in Pharmaceutical Formulations and Biological Samples Review

Penetapan Kadar Hidrokortison Asetat dalam Sediaan Krim Mengandung Pengawet Nipagin secara Spektrofotometri Derivatif Orde Pertama

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