Basic Principles and Problems of Haemocytometry

Lombarts, A.J.P.F.; Koevoet, A L; Leijnse, B.

doi:10.1177/000456328602300404

Cited by 22 publications

(16 citation statements)

References 56 publications

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“…At present, the complete blood cell count can be performed using an automated haematology analyser, which can increase the throughput of the test. Recently, new indices relating to erythrocytes (RDW, RDWa) and to platelets (PCT, MPV, PDW, LPCR) have been provided by hematologic analysers (Lombarts et al 1986). The current study is the first and a unique one that provided reference values for these new indices in midterm pregnant buffaloes.…”

Section: Resultsmentioning

confidence: 99%

Serum biochemical and haematological reference values for midterm pregnant buffaloes

Ellah

Hamed

Derar

2013

Journal of Applied Animal Research

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The current study aimed to establish reference values for pregnant Egyptian buffaloes (Bubalus bubalis) during the second trimester of pregnancy. All animals included in the study were non-lactating. The International Federation of Clinical Chemistry stated that at least 120 values were necessary to obtain reliable estimates for reference values. A total number of 134 clinically healthy midterm pregnant (3.5Á7.0 months) buffaloes (3Á8 years old) were included in this study. Animals were examined at the buffalo farms that belonged to the Assiut Governorate in the middle of Egypt. Three types of samples were collected: serum samples for biochemical analysis, whole blood samples for haematological analysis and faecal samples for parasitological examination. Animals that fit the inclusion criteria were included in this study. Biochemical analysis included serum total proteins, albumin, total globulins, a-, b-and g-globulin levels, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, creatine phosphokinase and lactate dehydrogenase activities, serum creatinine, blood urea nitrogen, total bilirubin, direct bilirubin, indirect bilirubin, sodium, potassium, chloride, magnesium, calcium, phosphorus, copper, zinc, iron, triglycerides, high-density lipoprotein, low-density lipoprotein, very low density lipoprotein and glucose levels. In addition, 20 haematological variables were measured. The 95% reference intervals were calculated by removing the upper and lower 2.5% of the intervals for each serum biochemical constituent to give 2.5 and 97.5 percentiles. Confidence intervals were calculated for each reference limit. Reference values from the current study were compared with the established values for cows. The current study is the first study to established reference values for the blood biochemical and haematological constituents in non-lactating buffaloes during the second trimester of pregnancy.

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Section: Resultsmentioning

confidence: 99%

Serum biochemical and haematological reference values for midterm pregnant buffaloes

Ellah

Hamed

Derar

2013

Journal of Applied Animal Research

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“…Recently, new indices related to erythrocytes (RDW, RDWa) and to platelets (PCT, MPV, PDW, LPCR) have been provided by haematology analysers (Lombarts, Koevoet & Leijnse 1986). The current study is unique in that it has provided reference values for these new indices in buffalo heifers.…”

Section: Discussionmentioning

confidence: 97%

Serum biochemical and haematological reference intervals for water buffalo (<i>Bubalus bubalis</i>) heifers

2014

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Based on a review of the literature, reference intervals for water buffalo (Bubalus bubalis) serum biochemistry and haematology have not previously been published. The current study was done to establish reference intervals for water buffalo heifers. The International Federation of Clinical Chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. A total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the study. Animals were examined at buffalo farms that belong to Assiut Governorate, Egypt. Three types of samples were collected: serum samples for biochemical analysis, whole blood samples for haematological analysis and faecal samples for parasitological examination. Animals that fitted the inclusion criteria were included in the study. Biochemical analysis included serum total proteins, albumin, total globulins, alpha, beta and gamma globulin levels, and aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, creatine phosphokinase and lactate dehydrogenase activity. In addition to the above, serum creatinine, urea, total bilirubin, direct bilirubin, indirect bilirubin, sodium, potassium, chloride, magnesium, calcium, phosphorus, copper, zinc, iron, triglycerides, high density lipoprotein, low density lipoprotein, very low density lipoprotein, glucose levels and 20 haematological variables were measured. The 95.0% reference intervals were calculated by removing the upper and lower 2.5% of the interval for each serum biochemical constituent to give the 2.5 and 97.5 percentiles. Confidence intervals were calculated for each reference limit. Reference intervals from the current study were compared with established values for cows. The current study is as far as could be determined the first that establishes reference intervals for the serum biochemical and haematological parameters in water buffalo heifers.

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“…The discrimination of this so-called three-part dif A rapid development has taken place in recent years c y tometer in the anal y sis of the P article size of P eĩ n the field of cell counting and cell differentiation of ri P heral blood cells ha^ " ow been im P r°ved > so that peripheral blood cells (1)(2)(3). The steadily increasing a five -P art ^ukocyte differentiation, m addition to demand for analyses has stimulated the introduction the usual Peelers of the whole blood cell count, r. r ,.…”

Section: Introductionmentioning

confidence: 99%

A New Development in Haematological Cell Counting: The Sysmex NE-8000, Automaton for Cell Count and Physical Five-Part Leukocyte Differentiation

Wersch

Bank²

1990

Clinical Chemistry and Laboratory Medicine

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We compared the results of the Sysmex NE-8000 with those of the Sysmex E-4000 and the Technicon H-6000. Both for the whole blood cell count and the leukocyte differentiation we obtained intraand inter-assay coefficients of variation fully comparable with those for other cell counters.The carry-over between samples was negligibly small. The linearity range was broad, which implies a universal application in routine haematological analysis. The numerical results from the Sysmex NE-8000 and the Technicon H-6000 corresponded closely, with exception of the mean corpuscular volume and the haematocrit, whereas these parameters showed excellent agreement between the NE-8000 and the E-4000. In the differentiation of leukocytes, the NE-8000 and H-6000 showed good correlation and conformity for lymphocytes, neutrophils and eosinophils; for monocytes and basophils the conformity was poor.We also found a good correlation between the NE-8000 and E-4000 for lymphocytes and neutrophils, whereas the correlation was less for the middle cell ratio and the sum of monocytes, eosinophils and basophils.Furthermore, we compared Receiver Operating Characteristic curves for the different leukocyte subtypes on the NE-8000, the H-6000 and the E-4000, and investigated the flagging frequency in inpatients, outpatients and blood donors.

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Basic Principles and Problems of Haemocytometry

Cited by 22 publications

References 56 publications

Serum biochemical and haematological reference values for midterm pregnant buffaloes

Serum biochemical and haematological reference values for midterm pregnant buffaloes

Serum biochemical and haematological reference intervals for water buffalo (<i>Bubalus bubalis</i>) heifers

A New Development in Haematological Cell Counting: The Sysmex NE-8000, Automaton for Cell Count and Physical Five-Part Leukocyte Differentiation

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