Basic Principles of Bioengineering and Regeneration

Aghaloo, Tara; Hadaya, Danny

doi:10.1016/j.coms.2016.08.008

Cited by 25 publications

(34 citation statements)

References 75 publications

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“…Alternatives to autogenous materials such as allograft, alloplast, and xenograft are used in grafting, but these carry their own disadvantage at the harvesting site in terms of biocompatibility, required scaffolding nature, and difficulty in obtaining a required anatomic geometry. 7 8 9 Biomaterials such as polylactic acid (PLA) and its copolymers with glycolic acid and other hydroxyl acids are of prime importance for orthopedic applications. 7 They act as a good medium where cellular proliferation, differentiation, collagen formation, and subsequent bone formation are achieved.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficacy of Three Different Bone Regeneration Materials: An Animal Study

et al. 2019

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Objective The proposed study aimed to evaluate and compare the bone regeneration between commercially available hydroxyapatite–β-tricalcium phosphate (Ossifi; Equinox, the Netherlands), powdered polylactic acid (powdered PLA; Sigma-Aldrich, United States), and three-dimensionally printed PLA (3D-printed PLA; Cubex, SC, United States) using 3D printer (Cube X trio) in an animal model. Materials and Methods Eighteen New Zealand rabbits were divided into three groups with six animals each. Platelet-rich fibrin (PRF) was collected from the venous blood and preserved. Bone defect (4 mm × 2 mm) without disturbing the bone marrow was created and filled with bone graft material (group 1–Ossifi, group 2–powdered PLA, and group 3–3D-printed PLA), over which PRF membranes were placed. The graft material and the barrier were stabilized using resorbable sutures, and all the animals were maintained for 4, 8, and 12 weeks, after which they were euthanized, and bone samples were retrieved. Retrieved bone samples were subjected to radiological and histological analysis. Results The radiographic and histological changes of 3D-printed PLA in comparison with other two materials (Ossifi and powdered PLA) seemed to have a significant difference. Conclusion 3D-printed PLA scaffolds showed positive signs of bone regeneration around the material in continuity defects. PLA material can be a promising alternative bone regenerative material.

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Section: Introductionmentioning

confidence: 99%

Comparison of the Efficacy of Three Different Bone Regeneration Materials: An Animal Study

et al. 2019

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“…Infiltrating inflammatory cells, such as neutrophils and macrophages, help in eliminating debris, microorganisms, and necrotic tissue, bringing the inflammatory phase to an end. These cells also secrete growth factors, cytokines, and chemokines that activate the proliferation phase (Aghaloo & Hadaya, 2017;Karppinen et al, 2019;Politis et al, 2016;Sculean et al, 2014;Shah et al, 2020).…”

Section: Tissue Repair: Wound Healing Process Of Soft Tissuementioning

confidence: 99%

“…During the proliferation phase, important processes such as angiogenesis, reepithelialization, and the formation of a new connective tissue matrix (collagen synthesis) occur (Aghaloo & Hadaya, 2017;Politis et al, 2016;Wang & Xu, 2020). Angiogenesis is known as the formation of new blood vessels from an existing vascular network.…”

Section: Tissue Repair: Wound Healing Process Of Soft Tissuementioning

confidence: 99%

Vascularization strategies in tissue engineering approaches for soft tissue repair

Masson-Meyers

Tayebi

2021

J Tissue Eng Regen Med

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Insufficient vascularization during tissue repair is often associated with poor clinical outcomes. This is a concern especially when patients have critical-sized injuries, where the size of the defect restricts vascularity, or even in small defects that have to be treated under special conditions, such as after radiation therapy (relevant to tumor resection) that hinders vascularity. In fact, poor vascularization is one of the major obstacles for clinical application of tissue engineering methods in soft tissue repair. As a key issue, lack of graft integration, caused by inadequate vascularization after implantation, can lead to graft failure. Moreover, poor vascularization compromises the viability of cells seeded in deep portions of scaffolds/graft materials, due to hypoxia and insufficient nutrient supply. In this article we aim to review vascularization strategies employed in tissue engineering techniques to repair soft tissues. For this purpose, we start by providing a brief overview of the main events during the physiological wound healing process in soft tissues. Then, we discuss how tissue repair can be achieved through tissue engineering, and considerations with regards to the choice of scaffold materials, culture conditions, and vascularization techniques. Next, we highlight the importance of vascularization, along with strategies and methods of prevascularization of soft tissue equivalents, particularly cellbased prevascularization. Lastly, we present a summary of commonly used in vitro methods during the vascularization of tissue-engineered soft tissue constructs.

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“…Instead, the lost tissue is replaced by a fibrous network with no restoration in form or function. In order to restore form and function, regeneration must take place by grafting the tissue [1]. Autogenous bone is the gold standard for regeneration of hard tissue defects.…”

Section: Introductionmentioning

confidence: 99%

Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells

Clark

Janorkar

2018

Bioengineering

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Human adipose-derived stem cells (hASCs) have been shown to differentiate down many lineages including endothelial lineage. We hypothesized that hASCs would more efficiently differentiate toward the endothelial lineage when formed as three-dimensional (3D) spheroids and with the addition of vascular endothelial growth factor (VEGF). Three conditions were tested: uncoated tissue culture polystyrene (TCPS) surfaces that induced a 2D monolayer formation; elastin-like polypeptide (ELP)-collagen composite hydrogel scaffolds that induced encapsulated 3D spheroid culture; and ELP-polyethyleneimine-coated TCPS surfaces that induced 3D spheroid formation in scaffold-free condition. Cells were exposed to endothelial differentiation medium containing no additional VEGF or 20 and 50 ng/mL of VEGF for 7 days and assayed for viability and endothelial differentiation markers. While endothelial differentiation media supported endothelial differentiation of hASCs, our 3D spheroid cultures augmented this differentiation and produced more von Willebrand factor than 2D cultures. Likewise, 3D cultures were able to uptake LDL, whereas the 2D cultures were not. Higher concentrations of VEGF further enhanced differentiation. Establishing angiogenesis is a key factor in regenerative medicine. Future studies aim to elucidate how to produce physiological changes such as neoangiogenesis and sprouting of vessels which may enhance the survival of regenerated tissues.

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Basic Principles of Bioengineering and Regeneration

Cited by 25 publications

References 75 publications

Comparison of the Efficacy of Three Different Bone Regeneration Materials: An Animal Study

Comparison of the Efficacy of Three Different Bone Regeneration Materials: An Animal Study

Vascularization strategies in tissue engineering approaches for soft tissue repair

Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells

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