Basic Proteins of Rodent Peripheral Nerve Myelin: Immunochemical Identification of the 21.5K, 18.5K, 17K, 14K, and P₂ Proteins

Abstract

Proteins in peripheral nervous system and central nervous system myelin and homogenates of sciatic nerve and brain from young and adult mice and rats were characterized with affinity-purified anti-P2 and anti-myelin basic protein sera after electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose sheets. Using this method we have identified a component of rodent peripheral nervous system myelin as P2 protein. Peripheral nervous system myelin also showed the presence of four basic proteins in addition to P2 protein. These were found to be analogous to the 14, 17, 18.5, and 21.5K species found in the central nervous system myelin. A number of high-molecular-weight proteins were also detected with anti-myelin basic protein serum in peripheral nervous system, as well as central nervous system myelin. In addition, we report the presence of a high-molecular-weight P2 cross-reactive protein in rodent brain stem homogenates, but not in central nervous system myelin. Key Words: Basic proteins--PNS myelin--CNS myelin--Immunocharacterization. Greenfield S. et al. Basic proteins of rodent peripheral nerve myelin: Immunochemical identification of the 21.5K, 18.5K, 17K, 14K, and P2 proteins.

Structure and Function of the Myelin Proteins: Current Status and Perspectives in Relation to Multiple Sclerosis

“…It also, contains an unusual tri-proline region [residues [99][100][101], which has been highlighted as an important structural feature similar to that contained in immunoglobulin G (IgG), and sequences homologous to cholera toxin A and B subunits [residues 102-118 and 67-77 in human MBP], which may possibly be involved in GM 1 ganglioside and GTP binding, as well as ADP ribosylation of MBP. In the PNS, four alternatively spliced forms of MBP are expressed in substantial amounts [138,139]. As has been reviewed in detail by Harauz et al [125], MBP interacts strongly with detergents and lipids, various metals and small ligands, and myelin and nonmyelin proteins that could be essential for the stability of myelin.…”

Identification of the New Isoforms of Mouse Myelin Basic Protein: The Existence of Exon 5a

“…1A). Immunoblot studies have indicated the existence of additional minor MBPs, such as a "20-kDa" form, in mouse (Greenfield et al, 1982;Carson et al, 1983;B a n d et al, 1987; for reviews, see Campagnoni, 1988;Campagnoni and Macklin, 1988). However, these minor MBP-related peptides have not been characterized intensively.…”

Correlation of three sciatic functional indices with histomorphometric findings in a rat sciatic nerve allograft repair model

“…20,[44][45][46] But it is not the total count or size of axons stained with different methods that determines the walking tracks, but the regeneration and firing of myelinated nerve fibers that innervate the muscle. [47][48][49] Hence, for histomorphological assessment in this study, it was feasible to use polyclonal antibodies with a high-affinity for marking only intact MBP of nerve fibers [50][51][52] and to choose randomly selected sampling grids as a nonbiased stereologic technique for quantitative morphological assessment avoiding sampling errors. 53 By using this method, blobs that could represent residual, unphagocytosed myelin were minimized and only healthy myelin sheaths were stained.…”