Basic Science Liver

Alagebrium Mitigates Metabolic Insults in High Carbohydrate and High Fat Diet Fed Wistar Rats

“…MF treatment also reduced TG and total cholesterol which agrees with Li et al, 47 attributing the lipid-lowering actions of MF to its important effects on bile acids, known to have lipid-lowing effects, which leads to improvement in glucose as well as lipid metabolism by their action on farnesoid X receptor (FXR). Regarding liver enzymes, treatment with ALA showed an improvement in serum levels of liver enzymes which agree with Fernando et al 25 who attributed the improvement in liver function to the inhibition the activated AGE/RAGE pathway in NAFLD that was initiated by the elevated AGE content in the diet enriched in fat and carbohydrate. MF also improved the abnormalities in liver enzymes and these results are in line with El-Lakkany et al 35 The effect of MF on ameliorating liver enzymes might account for the marked improvement of the liver histopathology and less fatty infiltration in hepatocytes as observed in the histopathological results of the current study.…”

“…50 Histopathological sections of rats received ALA was significantly improved. Fernando et al 25 reported similar findings. The author mentioned that the ability of ALA to scavenge the advanced lipoxidation end products (ALEs) that result from the action of reactive oxygen species on polyunsaturated lipids, which claimed to be the cause of fatty liver, is due to its ALEs cross-linkage breaking action.…”

“…The dose of ALA was selected according to the dose used in previous experimental studies. 22,[25][26][27] MF dose was selected according to the pharmacokinetic formula for humans and rats. 24 At the end of the experimental study the body weights of rats were estimated, and also SBP estimation was done by the Power Lab (4/35) data acquisition system (Australia) after anesthetizing rats with an intraperitoneal injection of pentobarbital (60 mg/kg, Sigma, St. Louis, USA).…”

“…Then, both FBG and insulin concentrations were used to calculate the index of insulin resistance (homeostasis model assessment [HOMA]-IR) according to this formula: HOMA IR = serum insulin (µIU/ml)×(blood glucose (mg/ dl)/405. 24,25 Serum total cholesterol and triglycerides determination Quantitative colorimetric determination method was used to estimate serum levels of total cholesterol (Catalog No: E-BC-K109-S, the intra-assay CV is 1.1% and the interassay CV is 2.8%) and triglycerides (Catalog No: E-BC-K261-S, the intra-assay CV is ≤5.0% and the inter-assay CV ≤8%) (Elabscience, 14780 Memorial Drive, Suite 216, Houston, Texas, USA).…”