2019
DOI: 10.1021/acsami.9b12169
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“Basicles”: Microbial Growth and Production Monitoring in Giant Lipid Vesicles

Abstract: We present an optimized protocol to encapsulate bacteria inside giant unilamellar lipid vesicles combined with a microfluidic platform for real-time monitoring of microbial growth and production. The microfluidic device allows us to immobilize the lipid vesicles and record bacterial growth and production using automated microscopy. Moreover, the lipid vesicles retain hydrophilic molecules and therefore can be used to accumulate products of microbial biosynthesis, which we demonstrate here for a riboflavin-prod… Show more

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Cited by 20 publications
(26 citation statements)
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“…To form GUVs, the oil-water transfer method was used. [41][42][43] A lipidstabilized water-in-oil emulsion was prepared by vigorously shaking 500 µL of lipids in mineral oil and 50 µL inner aqueous solution (see solutions) in a 1.5 mL centrifugation tube. A lipid-stabilized water-oil interface was created in another 1.5 mL centrifugation tube consisting of 500 µL buffer 1 (lower phase) and 200 µL of lipids in mineral oil (upper phase).…”
Section: Methodsmentioning
confidence: 99%
“…To form GUVs, the oil-water transfer method was used. [41][42][43] A lipidstabilized water-in-oil emulsion was prepared by vigorously shaking 500 µL of lipids in mineral oil and 50 µL inner aqueous solution (see solutions) in a 1.5 mL centrifugation tube. A lipid-stabilized water-oil interface was created in another 1.5 mL centrifugation tube consisting of 500 µL buffer 1 (lower phase) and 200 µL of lipids in mineral oil (upper phase).…”
Section: Methodsmentioning
confidence: 99%
“…MVVs are bilayer‐based multicompartment systems where a large or giant vesicle hosts one or more than one internal, smaller vesicles. A variation of these “vesicles‐inside‐vesicles” structures are “cells‐inside‐vesicles”, i. e., giant vesicles in which biological cells are entrapped [132–136] . They may be prepared by using some of the methods described in Chapter 2.…”
Section: Concluding Remarks and Outlookmentioning
confidence: 99%
“…They may be prepared by using some of the methods described in Chapter 2. The aim of investigating such biohybrid systems may lay in possible applications as organelles, [132,133] or for obtaining protected cells in a confined environment, whereby the giant vesicle membrane may serve as a protective layer if exposed to harsh environmental conditions, and with this promote cell growth in an unfriendly environment [134–136] …”
Section: Concluding Remarks and Outlookmentioning
confidence: 99%
“…These behaviors in a confined space are different from those observed conventionally. Juskova et al (2019) provided a novel method to monitor the real-time activity of bacterial growth inside giant unilamellar vesicles (GUVs) ( Figure 1A). They reported the encapsulation of single bacteria in small-volume GUVs (1-33 pL), followed by immobilization of the GUVs on a planar lipid bilayer membrane on a glass surface, under which photoelectronic detection could be applied at a single-cell level (Morita et al, 2018).…”
Section: Bacterial Growth and Proliferation In Confined Spacesmentioning
confidence: 99%
“…Scale bar, 25 μm. Adapted with permission from Juskova et al (2019) . Copyright 2019, American Chemical Society.…”
Section: Bacterial Growth and Proliferation In Confined Spacesmentioning
confidence: 99%