Basics of Self-Regeneration

Aires, Rita; Keeley, Sean D.; Sandoval-Guzmán, Tatiana

doi:10.1007/978-3-319-37076-7_66-1

Cited by 2 publications

(2 citation statements)

References 219 publications

(293 reference statements)

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“…GO analysis showed that, in both cases, there was an enrichment in genes related to processes of cell migration, cell adhesion, ECM disassembly and ECM organization (Fig. 3F, Table S4), all of which previously associated with the formation of a blastema (reviewed in Aires et al, 2020).…”

Section: Transcriptomic Analysis Of Mandible Regeneration After Later...mentioning

confidence: 79%

Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb

Kramer,

Aires,

Keeley

et al. 2024

Preprint

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The mandible plays an essential part in human life and, thus, defects in this structure can dramatically impair the quality of life in patients. Axolotls, unlike humans, are capable of regenerating their lower jaws; however, the underlying mechanisms and their similarity to those in limb regeneration are unknown. In this work, we used morphological, histological, and transcriptomic approaches to analyze the regeneration of lateral resection defects in the axolotl mandible. We found that this structure can regenerate all missing tissues in 90 days through gap minimization, blastema formation, and finally tissue growth, differentiation, and integration. Moreover, transcriptomic comparisons of regenerating mandibles and limbs showed that they share molecular phases of regeneration, that these similarities peak during blastema stages, and that mandible regeneration occurs at a slower pacing. Altogether, our study demonstrates the existence of a shared regenerative program used in two different regenerating body structures with different embryonic origins in the axolotl, and contributes to our understanding of the minimum requirements for a successful regeneration in vertebrates, bringing us closer to understand similar lesions in human mandibles.

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Section: Transcriptomic Analysis Of Mandible Regeneration After Later...mentioning

confidence: 79%

Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb

Kramer,

Aires,

Keeley

et al. 2024

Preprint

Self Cite

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“…Appendage regeneration has fascinated biologists for centuries, 1 as uncovering the molecular and cellular players regulating this process holds potential for therapies aimed at regenerating human limbs. While there are many different model organisms (e.g.…”

Section: Introductionmentioning

confidence: 99%

To regenerate or not to regenerate: Vertebrate model organisms of regeneration‐competency and ‐incompetency

Aztekin

Storer

2022

Wound Repair Regeneration

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Why only certain species can regenerate their appendages (e.g. tails and limbs) remains one of the biggest mysteries of nature. Unlike anuran tadpoles and salamanders, humans and other mammals cannot regenerate their limbs, but can only regrow lost digit tips under specific circumstances. Numerous hypotheses have been postulated to explain regeneration-incompetency in mammals. By studying model organisms that show varying regenerative abilities, we now have more opportunities to uncover what contributes to regeneration-incompetency and functionally test which perturbations restore appendage regrowth. Particularly, Xenopus laevis tail and limb, and mouse digit tip model systems exhibit naturally occurring variations in regenerative capacities. Here, we discuss major hypotheses that are suggested to contribute to regeneration-incompetency, and how species with varying regenerative abilities reflect on these hypotheses. | INTRODUCTIONAppendage regeneration has fascinated biologists for centuries, 1 as uncovering the molecular and cellular players regulating this process holds potential for therapies aimed at regenerating human limbs. While there are many different model organisms (e.g. zebrafish, salamander, deer) and appendage regeneration models (e.g. tail, digit tip, antler), each with unique characteristics, the bulk of our understanding of appendage regeneration has been derived from studies investigating amphibian limb and tail regeneration. 2 Amputations of appendages in these animals induce certain lineages to show a morphologically identified dedifferentiation phenotype and co-currently a simple wound epidermis covers the amputation plane. 3,4 This simple wound epithelium progresses into becoming a specialised wound epithelium called the apical-epithelial-cap (AEC). The AEC then functions as the key signalling centre during regeneration enabling expansion of lineage-restricted stem and progenitor populations, that are collectively termed the blastema. [5][6][7][8] The continuous interaction between the AEC and the blastema restores the lost appendage. Meanwhile, amputation of the mammalian limb fails to form an AEC or a blastema, and instead results in fibrotic scar formation and regenerative failure. 9Although mice, and in general amniotes including chickens 10 and lizards, 11 cannot perform limb regeneration, mouse digit tip amputations result in regrowth through formation of a blastema. 12,13 Interestingly, unlike limb regeneration in amphibians, there is no reported evidence that an AEC forms during digit tip regeneration, although the wound epidermis is suggested to be important for digit tip regrowth. 7 Instead of a signalling center AEC, nail bed stem cells were proposed to act as a signalling center population enabling expansion of progenitor cells during digit tip regeneration. 14,15 Nonetheless, it remains unclear why mammals, and amniotes in general, cannot perform full limb regeneration.

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Basics of Self-Regeneration

Cited by 2 publications

References 219 publications

Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb

Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb

To regenerate or not to regenerate: Vertebrate model organisms of regeneration‐competency and ‐incompetency

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