Basil (<i>Ocimum basilicum</i>), a new host of <i>Pepino mosaic virus</i>

Davino, Salvatore; Accotto, Gian Paolo; Masenga, V.; Torta, Livio; Davino, M.

doi:10.1111/j.1365-3059.2009.02026.x

Cited by 23 publications

(16 citation statements)

References 4 publications

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“…This is the first observation and reporting in the continent. Similar pathological cases reported 2009 in other continent 11,12 indicated the authenticity of the current situation in Africa.…”

Section: Resultssupporting

confidence: 83%

Untitled

2013

IJPSR

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Section: Resultssupporting

confidence: 83%

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2013

IJPSR

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“…The recent advancement of molecular diagnostic systems during the past few years has enabled rapid diagnosis of TYLCD in field. Similarly several conventional PCR and qPCR-based techniques have improved remarkably for the detection of various virus species (Accotto et al, 2000;Gorsane et al, 2005;Davino et al, 2009). The development of fluorescent methods for PCR and also the instruments like real-time which can monitor the amplification process is considered as a significant improvement in molecular biology.…”

Section: Discussionmentioning

confidence: 99%

Real-time qPCR Assay for the TYLCV Titer in Relation to Symptoms-Based Disease Severity Scales

Ammara

Al-Sadi²,

Al-Shihi

et al. 2017

IJAB

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During the last few decades, Tomato yellow leaf curl disease (TYLCD) has resulted in heavy yield losses and increased incidences in tropical and subtropical regions. In Oman, six different species of begomoviruses were found to be associated with TYLCD. However, Tomato yellow leaf curl virus-Oman (TYLCV-OM) was found to be the most widely distributed species in the whole country. A real-time quantitative PCR (qPCR) assay with an internal control [tomato elongation factor 1(EF1)] was developed for TYLCV-OM, based on SYBRGreen chemistry for the rapid detection and quantification of the virus. This assay was carried out on field samples showing symptoms according to The World Vegetable Center (AVRDC) disease severity scales. The test was used to establish a relationship between virus load and phenotypic symptoms. Viral copies of 2.88 × 10 9 were detected in field infected tomato plants showing symptom severity according to AVRDC scale '3' (i.e. severe leaf curling and yellowing of leaves). The copy number of virus decreased with the relative decrease in symptom severity scale. Tomato plants exhibiting scale '1' and '2' have 7.7 × 10 4 and 7.47 × 10 6 viral copies, respectively. Very low copy number of the virus (564) was detected in tomato plants showing symptoms at a severity scale of '0', which were apparently symptomless. However, tomato plants developed by tissue culture in sterilized conditions, which were used as a negative control, did not show the presence of the virus. The developed qPCR assay could detect as low as 18 fg (femto gram) of virus from total nucleic acid equivalent to approximately 30 genomic units. This quantitative assay can be used to determine virus titer, in breeding programs for development of virus resistant plants and for epidemiological surveys to monitor viral populations and their intensities.

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“…Figure 2 Impatiens necrotic spot virus (genus Tospovirus), a major pathogen of flower crops (Daughtrey et al, 1997), has recently been reported also to infect vegetables and other edible crops including spinach, lettuce, pepper, peanut and potato (Koike et al, 2008, Liu et al, 2009 Davino et al (2009), the plants were also tested for this virus. Total RNA was extracted from leaves showing symptoms using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany).…”

Section: Figurementioning

confidence: 99%

First report of Impatiens necrotic spot virus on Ocimum basilicum, Eruca sativa and Anthriscus cerefolium in Austria

Grausgruber-Gröger

2012

New Disease Reports

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Basil (Ocimum basilicum), a new host of Pepino mosaic virus

Cited by 23 publications

References 4 publications

Untitled

Untitled

Real-time qPCR Assay for the TYLCV Titer in Relation to Symptoms-Based Disease Severity Scales

First report of Impatiens necrotic spot virus on Ocimum basilicum, Eruca sativa and Anthriscus cerefolium in Austria

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