Basis and monitoring of methoxyfenozide resistance in the South American tomato pinworm Tuta absoluta

Langa, Teófilo Paulo; Dantas, Kayo César Tavares; Pereira, Daniel Lima; Oliveira, Marcos de; Ribeiro, Lílian M.S.; Siqueira, Herbert A.A.

doi:10.1007/s10340-021-01378-z

Cited by 11 publications

(8 citation statements)

References 55 publications

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“…There have been several efforts to monitor the global invasions of T. absoluta over the past decade (Campos et al, 2017 ; Han et al, 2018 ; Li et al, 2021 ). Chemical control is the primary measure to manage T. absoluta in both native and invaded areas (Silva et al, 2018 ; Langa et al, 2021 ). However, the large use of chemical pesticides led to environmental pollution and food safety challenges (Machekano et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, various pesticide-resistant strains have emerged in different locations (Zappal et al, 2013 ; El-Arnaouty et al, 2014 ; Biondi et al, 2015 ). Alternative methods of management, such as using its natural enemies (El-Arnaouty et al, 2014 ; Biondi et al, 2015 ; Silva et al, 2018 ; Langa et al, 2021 ) and pheromones, have proven somewhat effective in T. absoluta population suppression (Aksoy and Kovanci, 2016 ; Mirhosseini et al, 2019 ), but still requires a more efficacious management strategy to limit its havoc on crop yield.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation

Chen

Zhang

et al. 2021

Front. Plant Sci.

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The South American tomato pinworm, Tuta absoluta, is one of the most destructive insect pests in Solanaceae crops, particularly in tomatoes. Current methods of management have proven somewhat effective but still require a more efficacious management strategy to limit its havoc on crop yield. Tomato is much more predisposed to T. absoluta as compared with other plants such as eggplants, but the underlying causes have not been fully determined. We conducted this study to unravel the volatile organic compounds (VOCs) and primary/secondary metabolites that account for the differential response of tomatoes and eggplants to T. absoluta infestation. We performed widely targeted comparative metabolome and volatilome profiling by ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC-MS), respectively, on eggplants and tomatoes under control and T. absoluta infestation conditions. Overall, 141 VOCs and 797 primary/secondary metabolites were identified, largely dominated by aldehyde, alcohols, alkanes, amine, aromatics, a heterocyclic compound, ketone, olefin, phenol, and terpenes. Most of the VOCs and primary/secondary metabolites from the terpene class were largely differentially regulated in eggplants compared with tomatoes. Eggplants emitted several compounds that were lower or completely absent in tomatoes either under control conditions or after T. absoluta infestation. The results from an electroantennogram showed that 35 differentially accumulated VOCs could elicit female T. absoluta response, implying that these volatile compounds significantly alter the behavior of this pest. These findings demonstrated that differentially accumulated metabolites and volatile compounds play major roles in eggplant resistance to T. absoluta infestation as these compounds were regulated upon attack by T. absoluta. Our findings can assist in integrated pest management efforts by developing appropriate control measures against T. absoluta in Solanaceae production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation

Chen

Zhang

et al. 2021

Front. Plant Sci.

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“…Because of the severe economic of T. absoluta , as well as the reported development of pesticide resistance ( Roditakis et al, 2015 , 2017 ; Siqueira et al, 2020 ; Langa et al, 2021 ), the species has been intensively studied, especially in regard to the identification of RNAi control targets. However, the limited efficacy duration of RNA interference (RNAi) ( Rahmani and Bandani, 2021a , b ) was constrained to use broadly it to do the functional genomic studies of T. absoluta .…”

Section: Introductionmentioning

confidence: 99%

First Report on CRISPR/Cas9-Based Genome Editing in the Destructive Invasive Pest Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Wang

et al. 2022

Front. Genet.

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The tomato leaf miner Tuta absoluta (Meyrick) is one of the world’s most destructive pests of tomato, and because of its severe economic impacts, as well as the development of pesticide resistance, the species has been intensively studied, especially in regard to the identification of targets for T. absoluta control. However, functional genomic studies of T. absoluta have been constrained by a lack of effective genetic tools. Therefore, the aim of the present study was to develop a CRISPR/Cas9 zygote microinjection protocol for generating heritable mutations in T. absoluta, using the ommochrome synthesis gene cinnabar as an easily evaluated target gene. The injection of fertilised eggs with Cas9 protein and four sgRNAs, which targeted cinnabar exon 3, resulted in a mutagenesis rate of 31.9% for eggs reaching adulthood, and cinnabar mutagenesis resulted in either red or mosaic eye colour phenotypes. As such, this study is the first to report a complete and detailed CRISPR/Cas9 workflow for the efficient genome editing of the globally important invasive pest T. absoluta. The application of this robust genome-editing tool to T. absoluta will greatly facilitate the discovery of suitable RNAi control targets and the subsequent development of novel control strategies.

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“…In recent research, the nano-formulation of insecticide has proven the higher efficiency for insect control with lesser harmful effects on the environment as compared with technical insecticide [ 26 ]. Methoxyfenozide is a diacylhydrazine insecticide, highly effective against lepidopteran larvae, chemically safe for environment and is used to control many insects [ 27 , 28 ]. The active molecules can potentially mimic the action of two insect hormones, the sesquiterpenoid juvenile hormone (JH) and the steroidal molting hormones, 20-hydroxyecdysone (20E), which regulate different stages of development and growth of insects [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Bilal

Sial

Cao

et al. 2022

IJMS

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The diamond back moth, Plutella xylostella, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO2 NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO2 NPs-70 nm, Me@FL-SiO2 NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO2 NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO2 NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC50 value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO2 NPs-70 nm, and 15 mg/L for Me@FL-SiO2 NPs-150 nm after 72 h exposure, respectively. After calculating the LC50, the results predicted that Me@FL-SiO2 NPs-70 nm and Me@FL-SiO2 NPs-150 nm exhibited better insecticidal activity against P. xylostella than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of P. xylostella were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO2 NPs into the midgut of P. xylostella was confirmed by confocal laser scanning microscope (CLSM). For comparison, P. xylostella under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO2 NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.

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Basis and monitoring of methoxyfenozide resistance in the South American tomato pinworm Tuta absoluta

Cited by 11 publications

References 55 publications

Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation

Comprehensive Metabolome and Volatilome Analyses in Eggplant and Tomato Reveal Their Differential Responses to Tuta absoluta Infestation

First Report on CRISPR/Cas9-Based Genome Editing in the Destructive Invasive Pest Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

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