2012
DOI: 10.1113/jphysiol.2012.236919
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Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu‐3

Abstract: Key points• The companion paper provided evidence for basolateral anion exchange during cAMP stimulation of chloride and fluid secretion by Calu-3 monolayers; however, the molecular basis of this transport was not identified.• To test the role of AE2, an anion exchanger expressed at the basolateral membrane of Calu-3 and many other epithelial cells, we used lentivirus-mediated RNA interference to generate a stable Calu-3 AE2 knock-down cell line and characterized its fluid and anion transport properties.• AE2 … Show more

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Cited by 24 publications
(28 citation statements)
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“…Previous studies on Calu-3 cells indicate that the Cl Ϫ /HCO 3 Ϫ exchanger AE2 is expressed at the basolateral membrane (16,34,55). To confirm this, we studied the effect of removing Cl Ϫ from the basolateral perfusate in the presence of HCO 3 Ϫ .…”
Section: Basolateral CL ϫ /Hco 3 ϫ Exchangementioning
confidence: 85%
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“…Previous studies on Calu-3 cells indicate that the Cl Ϫ /HCO 3 Ϫ exchanger AE2 is expressed at the basolateral membrane (16,34,55). To confirm this, we studied the effect of removing Cl Ϫ from the basolateral perfusate in the presence of HCO 3 Ϫ .…”
Section: Basolateral CL ϫ /Hco 3 ϫ Exchangementioning
confidence: 85%
“…AE2 is ubiquitously expressed at the basolateral membrane of secretory epithelial cells (49), and its presence in Calu-3 cells is well documented (16,34). While it has an important role protecting cells from intracellular alkalinization, it can also be involved in cell volume regulation and transepithelial Cl Ϫ secretion.…”
Section: Role Of Basolateral Ae2 In CL ϫ and Hco 3 ϫ Secretionmentioning
confidence: 98%
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“…As noted earlier, both of these consequences can contribute towards the vectoral transport of NaCl, together with osmotically obligated H 2 O, across epithelia. In airway, duodenal, and colonic epithelia, the functionally coupled action of AE2 and an NCBT (e.g., AE2 and NBCe1 in FIGURE 22) performs, along with NKCC1, Na ϩ and Cl Ϫ influx across the basolateral membrane that supports CFTR-mediated Cl Ϫ secretion across the apical membrane (312,397,1019). Knocking out murine AE2 or NBCe1 is associated with a presumably compensatory increase in NKCC activity in intestinal epithelia (312,313).…”
Section: E) Ae2: Hcomentioning
confidence: 98%
“…2,4,5 Of these, AE2 (SLC4A2) is widely expressed in most cells, including epithelial cells such as airway epithelia, proximal colon, and salivary glands cells. [6][7][8] AE activity is regulated by many signals in epithelial cells. At the cellular level, muscarinic receptor stimulation enhances AE activity in salivary acinar cells.…”
Section: Introductionmentioning
confidence: 99%