Basophil Expansion Protects Against Invasive Pneumococcal Disease in Mice

Bischof, Andrea; Brumshagen, Christina; Ding, Nadine; Kirchhof, Gabriele; Briles, David E.; Gessner, Johannes; Welte, Tobias; Mack, Matthias; Maus, Ulrich A.

doi:10.1093/infdis/jiu056

Cited by 8 publications

(7 citation statements)

References 33 publications

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“…Recombinant PspA was produced using the Escherichia coli strain BL21STAR(DE) carrying the pUAB055 expression vector, which was generated as previously described . Briefly, PspA was purified through a nickel‐charged resin bed (Novagen, Madison, WI, USA) via affinity chromatography.…”

Section: Methodsmentioning

confidence: 99%

Nasopharyngeal colonization with Streptococcus pneumoniae triggers dendritic cell dependent antibody responses against invasive disease in mice

et al. 2017

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Nasopharyngeal colonization with Streptococcus pneumoniae (Spn) is an important precondition for the development of pneumococcal pneumonia. At the same time, nasopharyngeal colonization with Spn has been shown to mount adaptive immune responses against Spn in mice and humans. Cellular responses of the nasopharyngeal compartment, including the nasal-associated lymphoid tissue, to pneumococcal colonization and their importance for developing adaptive immune responses are poorly defined. We show that nasopharyngeal colonization with S. pneumoniae led to substantial expansion of dendritic cells (DCs) both in nasopharyngeal tissue and nasal-associated lymphoid tissue of mice. Depletion of DCs achieved by either diphtheria toxin (DT) treatment of chimeric zDC mice, or by use of FMS-like tyrosine kinase 3 ligand (Flt3L) KO mice exhibiting congenitally reduced DC pool sizes, significantly diminished antibody responses after colonization with Spn, along with impaired protective immunity against invasive pneumococcal disease. Collectively, the data show that classical DCs contribute to pneumococcal colonization induced adaptive immune responses against invasive pneumococcal disease in two different mouse models. These data may be useful for future nasopharyngeal vaccination strategies against pneumococcal diseases in humans.

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Section: Methodsmentioning

confidence: 99%

Nasopharyngeal colonization with Streptococcus pneumoniae triggers dendritic cell dependent antibody responses against invasive disease in mice

et al. 2017

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“…Basophils synergize with dendritic cells to promote Th2 cell differentiation [23-25] and present peptides and haptens to CD4 + T cells [24]. Additionally, basophils have been implicated in the pathogenesis of lupus nephritis [26] and in regulating immune responses to bacterial infections [27,28]. Increased numbers of circulating basophils are often associated with myelodysplastic syndromes, acute and chronic myeloid leukemia and reduced survival of these patients [29-32].…”

Section: Basophil and Mast Cell Biologymentioning

confidence: 99%

Transcriptional regulation of mast cell and basophil lineage commitment

Huang

Liu

2016

Semin Immunopathol

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Basophils and mast cells have long been known to play critical roles in allergic disease and in immunity against parasitic infection. Accumulated evidence supports that basophils and mast cells also have important roles in immune regulations, host defense against bacteria and viruses, and autoimmune diseases. However, origin and molecular regulation of basophil and mast cell differentiation remain incompletely understood. In this review, we focus on recent advances in the understanding of origin and molecular regulation of mouse and human basophil and mast cell development. A more complete understanding of how basophils and mast cells develop at the molecular level will lead to development of interventions that are more effective in achieving long-term success.

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“…Recombinant pneumococcal surface protein A (PspA) was produced by E. coli strain BL21STAR(DE) carrying the pUAB055 expression vector, which was generated and purified as previously described . Determination of PspA‐specific antibody levels in serum of naïve or previously colonized WT, CD103 KO and Batf3 KO mice was performed essentially as described recently .…”

Section: Methodsmentioning

confidence: 99%

Colonization‐induced protection against invasive pneumococcal disease in mice is independent of CD103 driven adaptive immune responses

et al. 2018

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Nasopharyngeal colonization with Streptococcus pneumoniae (the pneumococcus) is known to mount protective adaptive immune responses in rodents and humans. However, the cellular response of the nasopharyngeal compartment to pneumococcal colonization and its importance for the ensuing adaptive immune response is only partially defined. Here we show that nasopharyngeal colonization with S. pneumoniae triggered substantial expansion of both integrin αE (CD103) positive dendritic cells (DC) and T lymphocytes in nasopharynx, nasal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) of WT mice. However, nasopharyngeal de-colonization and pneumococcus-specific antibody responses were similar between WT and CD103 KO mice or Batf3 KO mice. Also, naïve WT mice passively immunized with antiserum from previously colonized WT and CD103 KO mice were similarly protected against invasive pneumococcal disease (IPD). In summary, the data show that CD103 is dispensable for pneumococcal colonization-induced adaptive immune responses in mice.

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Basophil Expansion Protects Against Invasive Pneumococcal Disease in Mice

Abstract: This is the first study to find that a single transfusion of basophils is sufficient to boost protein-based memory responses against pneumococcal protein antigens, thereby providing significant protection against IPD in mice.

Cited by 8 publications

References 33 publications

Nasopharyngeal colonization with Streptococcus pneumoniae triggers dendritic cell dependent antibody responses against invasive disease in mice

Nasopharyngeal colonization with Streptococcus pneumoniae triggers dendritic cell dependent antibody responses against invasive disease in mice

Transcriptional regulation of mast cell and basophil lineage commitment

Colonization‐induced protection against invasive pneumococcal disease in mice is independent of CD103 driven adaptive immune responses

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