Basophil Releasability in Patients with Hymenoptera Venom Allergy

Radermecker, Maurice; Leclercq, M.; Mariz, Segundo D.; Louis, Renaud

doi:10.1159/000236459

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…Moreover, when the cell reactivity to anti-lgE was compared with that to Derp K a highly significant correlation was observed between them (r,-0.690, P= 0.0012). The observations are compatible with previous studies (18,23). The results seem to indicate that other modulatory factors may be involved in the reactivity to immunologic stimulation.…”

Section: Discussionsupporting

confidence: 92%

IgE‐mediated basophil releasability is influenced by intrinsic factors and by IgE on the cell surface

Mita

Yasueda

Ishii

et al. 1995

Allergy

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Experiments were done to clarify the mechanisms associated with releasability of histamine. First, washed leukocytes from 23 asthmatic patients sensitive to mite allergen were challenged with Der p 1, a major allergen isolated from Dermatophagoides pteronyssinus, or anti-IgE. A significant correlation was observed between the ratio of Der p 1-specific IgE titer to total IgE level (S/T) in the patient's plasma and either the reactivity (maximal percentage of histamine release; rs = 0.514, P = 0.016, n = 23) or the sensitivity (the minimum allergen concentration required to achieve 25% histamine release; rs = -0.790, P = 0.0002) to Der p 1. Additionally, the reactivity to Der p 1 was significantly correlated with that to anti-IgE (rs = 0.690, P = 0.0012), indicating that an intrinsic cellular property may be one of the contributing factors in immunologic histamine release. In a second series of experiments, sinus mast cells were passively sensitized with immunoglobulins prepared from the patient's plasma. A statistically significant correlation was found between either the reactivity or the sensitivity to Der p 1 and S/T, thus indicating that S/T is an indicator of the releasability of histamine. When basophils or mast cells were passively sensitized with mouse IgE and subsequently stimulated with antimouse IgE, the reactivity to antihuman IgE was significantly correlated with that to antimouse IgE (rs = 0.966, P = 0.0023, n = 11). These observations suggest that an intrinsic cellular property regulates reactivity in immunologic histamine release. Taken together, our results suggest that an intrinsic cellular property, as well as specific IgE antibody levels on the cell surface, is an important factor in determining histamine release in response to IgE-dependent activation.

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Section: Discussionsupporting

confidence: 92%

IgE‐mediated basophil releasability is influenced by intrinsic factors and by IgE on the cell surface

Mita

Yasueda

Ishii

et al. 1995

Allergy

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“…Other in vitro tests When venom skin tests and the measurement of venom‐specific IgE antibodies in serum by RAST or an equivalent method yield negative results in patients with a history of a systemic anaphylactic sting reaction, additional in vitro tests may be used to demonstrate immunologic sensitization (like immunoblotting, the basophil histamine release test, basophil activation test and leukotrine release test) (122–126).…”

mentioning

confidence: 99%

Diagnosis of Hymenoptera venom allergy

Bilò¹,

Ruëff

Mosbech³

et al. 2005

Allergy

505

439

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The first EAACI position paper on immunotherapy with Hymenoptera venoms was published in 1987 (1). Six years later a revised version appeared (2). As then many papers on the diagnosis and treatment of Hymenoptera venom allergy have been published, making a review of the last EAACI position paper necessary. This paper focuses on Hymenoptera venom allergy, as allergic reactions caused by stings of insects other than Hymenoptera are rare and standardized extracts for the diagnosis and treatment of allergic reactions to nonHymenoptera insects are not available (3). TaxonomyMost authors follow the Chinery classification (4), although over the last few years a few minor changes have been introduced. Aculeatae are a suborder of Hymenoptera (Fig. 1).The family Apidae consists of the honeybees (Apis mellifera) who are brown in color and moderately hairy and the bumblebees (Genus Bombus) which are bigger than honeybees, much more hairy and characterized by distinct yellow or white bands on their abdomen. Vespidae are divided into the Vespinae and Polistinae subfamilies, with differences at the junction of the thorax and abdomen. Vespinae have a truncated junction while Polistinae are more oval in shape. Vespidae are almost hairless and have black and yellow striped abdomens.Vespula, Dolichovespula and Vespa make up the three genera of the Vespinae. Vespula (called wasps in Europe, yellow jackets in the USA) are the most important species in Europe. The Vespula spp. (V. germanica and The purpose of diagnostic procedure is to classify a sting reaction by history, identify the underlying pathogenetic mechanism, and identify the offending insect. Diagnosis of Hymenoptera venom allergy thus forms the basis for the treatment. In the central and northern Europe vespid (mainly Vespula spp.) and honeybee stings are the most prevalent, whereas in the Mediterranean area stings from Polistes and Vespula are more frequent than honeybee stings; bumblebee stings are rare throughout Europe and more of an occupational hazard. Several major allergens, usually glycoproteins with a molecular weight of 10-50 kDa, have been identified in venoms of bees, vespids. and ants. The sequences and structures of the majority of venom allergens have been determined and several have been expressed in recombinant form. A particular problem in the field of cross-reactivity are specific immunoglobulin E (IgE) antibodies directed against carbohydrate epitopes, which may induce multiple positive test results (skin test, in vitro tests) of still unknown clinical significance. Venom hypersensitivity may be mediated by immunologic mechanisms (IgE-mediated or non-IgE-mediated venom allergy) but also by nonimmunologic mechanisms. Reactions to Hymenoptera stings are classified into normal local reactions, large local reactions, systemic toxic reactions, systemic anaphylactic reactions, and unusual reactions. For most venom-allergic patients an anaphylactic reaction after a sting is very traumatic event, resulting in an altered health-related quality of life. Risk f...

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“…In about 15% of subjects allergic to HV, basophils do not release histamine in the presence of antigen [25,51]. Consequently, the clinical history should drive the diagnosis of insect sting allergy and the indication for SIT [48].…”

Section: Discussionmentioning

confidence: 99%

Approaches to the Management of Presumed Immediate Hymenoptera Venom Allergy and Non-Detectable IgE

Mingomataj¹,

Bakiri²

2010

TOALLJ

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Objective: To provide a comprehensive evaluation in patients with a convincing history of immediate insect allergy but negative skin test and/or specific IgE results, adequately addressing the question of how best to manage them. Data sources: Observational peer-reviewed studies and case reports were searched on Pub-Med database from 1998 up to March 2009 using the following keywords: Hymenoptera Allergy & Negative IgE (Negative Skin Tests). Study selection: Studies on supplemental diagnostic tests that provided data from patients with immediate hymenoptera allergy but negative conventional tests results to the offending allergens were selected. In this work, we also included studies providing additional relevant information regarding this issue. Results: Among 43 identified papers only 9 of them presented relevant original data, while the other papers were reviews. In the majority of the cases, the culprit insect was identified with in vitro tests such as Basophil Activation Test, Cellular Allergen Stimulation Test or Western blot, whereas in vivo (less frequently) with sting challenge or dialyzed venom skin test. Conclusions: The management of patients with a convincing history of immediate insect allergy but negative conventional test results requires an adaption of the guidelines including an incorporation of the novel diagnostic tools. Although cellular tests represent equivalent sensitivity and superior specificity as compared with standard ones, these tests still remain supplementary diagnostic tools. In a minority of cases (especially in the developing countries where cellular tests cannot be performed), venom immunotherapy in adult subjects could be taken into account based solemnly on the history of a clear patient’s identification of the culprit insect.

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Basophil Releasability in Patients with Hymenoptera Venom Allergy

Cited by 7 publications

References 9 publications

IgE‐mediated basophil releasability is influenced by intrinsic factors and by IgE on the cell surface

IgE‐mediated basophil releasability is influenced by intrinsic factors and by IgE on the cell surface

Diagnosis of Hymenoptera venom allergy

Approaches to the Management of Presumed Immediate Hymenoptera Venom Allergy and Non-Detectable IgE

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