Batch and continuous cultures of Mannheimia succiniciproducens MBEL55E for the production of succinic acid from whey and corn steep liquor

Abstract

Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is able to produce a large amount of succinic acid in a medium containing glucose, peptone, and yeast extract. In order to reduce the cost of the medium, whey and corn steep liquor (CSL) were used as substrates for the production of succinic acid by M. succiniciproducens MBEL55E. Anaerobic batch cultures of M. succiniciproducens MBEL55E in a whey-based medium containing CSL resulted in the production of succinic acid with a yield of 71% and productivity of 1.18 g/l/h, which are similar to those obtained in a whey-based medium containing yeast extract (72% and 1.21 g/l/h). Anaerobic continuous culture of M. succiniciproducens MBEL55E in a whey-based medium containing CSL resulted in a succinic acid yield of 69% and a succinic acid productivity as high as 3.90 g/l/h. These results show that succinic acid can be produced efficiently and economically by M. succiniciproducens MBEL55E from whey and CSL.

Continuous succinic acid production from xylose by Actinobacillus succinogenes

“…Various microbial platforms have been explored for bio-production of SA with the best bench-scale performance achieved by Actinobacillus succinogenes [11][12][13][14], Anaerobiospirillum succiniciproducens [15,16], Mannheimia succiniciproducens [17][18][19], and engineered strains of Escherichia coli [20][21][22].…”

Continuous succinic acid fermentation by Actinobacillus succinogenes

“…succiniciproducens was first isolated [13][14][15][16][17][18][19]. Apart from normal suspended cell systems ('chemostat'), membrane systems with cell recycle as well as biofilm reactors were used in an attempt to enhance productivity [11][12][13]17].…”

Improved Succinic Acid Production in the Anaerobic Culture of an Escherichia coli pflB ldhA Double Mutant as a Result of Enhanced Anaplerotic Activities in the Preceding Aerobic Culture

“…Several bacteria, such as Mannheimia succiniciproducens (14), Actinobacillus succinogenes (31), and Anaerobiospirillum succiniciproducens (23), are effective natural succinate producers with high productivity and tolerance to osmotic pressure caused by glucose and succinic acid. Recently studies on metabolic engineering of Escherichia coli for succinic acid production have been carried out.…”