Batch and fed-batch production of probiotic biomass and nisin in nutrient-supplemented whey media

Malvido, Mónica Costas; González, Elisa Alonso; Tantaleán, Delicia L Bazán; Jácome, Ricardo J. Bendaña; Guerra, Nelson Pérez

doi:10.1007/s42770-019-00114-1

Cited by 21 publications

(13 citation statements)

References 21 publications

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“…In these growth phases, the biomass concentration increased to 3.11 (0–72 h) and 8.03 g/L (72–252 h) but then decreased to 6.16 g/L (252–348 h). This behavior has been observed before in realkalized fed-batch cultures with Pediococcus acidilactici NRRL B-5627 [ 30 ], Lactococcus lactis CECT 539 [ 17 , 18 , 29 , 31 , 32 ] and Lactobacillus casei CECT 4043 [ 2 ], which are species present in the kefir grains [ 33 , 34 , 35 ].…”

Section: Resultssupporting

confidence: 68%

“…The results obtained in the fermentation of whey inoculated with 1% ( w / v ) of kefir grains plus 1% ( v / v ) of the fermented whole milk used in the activation of these kefir grains (fermentation I) are shown in Figure 1 . As can be seen, the evolution of the culture variables: pH, free biomass, nutrients (lactose, glucose, galactose and proteins) and products (lactic and acetic acids, ethanol and glycerol) in this culture showed profiles different from those of fermentation with a pure culture [ 17 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

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Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

Agregán

González

Mejuto

et al. 2021

IJMS

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Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation of whey with a combination of kefir grains, AGK1, and the fermented whole milk used to activate these kefir grains. The probiotic culture obtained was characterized by high levels of biomass (8.03 g/L), total viability (3.6 × 108 CFU/mL) and antibacterial activity (28.26 Activity Units/mL). Some probiotic properties of the probiotic culture were investigated in vitro, including its survival at low pH values, under simulated gastrointestinal conditions, after freezing in skim milk at −20 °C, and in the commercial feed during storage at room temperature. The viable cells of lactic and acetic acid bacteria and yeasts exhibited higher tolerance to acidic pH and simulated gastrointestinal conditions when the cells were protected with skim milk and piglet feed, compared with washed cells. The results indicated the feasibility of producing a probiotic product at a low cost with a potential application in animal feed.

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Section: Resultssupporting

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

Agregán

González

Mejuto

et al. 2021

IJMS

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“…Traditional batch and fed-batch fermentation with suspended cells are almost solely used in industrial operations for food culture production, including probiotics. In batch and re-alkalized fed-batch fermentation in diluted whey (DW) media supplemented with de Man, Rogosa, and Sharpe (MRS) broth nutrients (except glucose and Tween 80), the production of a highly concentrated probiotic preparation of Lactococcus lactis CECT 539 was investigated by Malvido et al (2019). The maximum concentrations of probiotic biomass (5.98 g/L) and nisin (258.47 BU/mL) were achieved in the fed-batch culture using DW100 medium, which was obtained at lower production costs than those projected for the fed-batch culture in DW medium.…”

Section: Manufacturing Of Probiotics Biomassmentioning

confidence: 99%

Probiotics media: significance, challenges, and future perspective - a mini review

Kumar

Naik

Kumar

et al. 2022

Food Prod Process and Nutr

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The health benefits associated with probiotics have increased their application in pharmaceutical formulations and functional food development. High production of probiotic biomass requires a cost-effective production method and nutrient media optimization. The biomass production of probiotics can be enhanced by optimizing growth parameters such as substrate, pH, incubation time, etc. For economical industrial production of probiotic biomass, it is required to design a new medium with low cost. Wastes from the food industries are promising components for the development of the low-cost medium. Industrial wastes such as cheese whey and corn steep liquor are excellent examples of reliable sources of nitrogen for the biomass production of probiotic bacteria. The increased yield of biomass reduced the cost of production. This review focuses on the importance of probiotic media for biomass production and its challenges. Graphical Abstract

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“…The use of whey proteins for the growth of biotechnologically interesting microorganisms is well established [16]. For example, the probiotic strain L. casei is a well-known case study for the production of biomass [11] and other antimicrobial products such as nisin and bacteriocins [12,13]. Since lactic acid bacteria present specific and critical nutritional requirements, often supplementation with growth factors, vitamins and amino acids is necessary.…”

Section: Evaluation Of Ultrafiltered Spray-dried Whey As Substrate Fo...mentioning

confidence: 99%

“…Due to the high lactose content in addition to proteins, whey, very often in combination with other medium components, was also used as a substrate for the cultivation of diverse microorganisms [10]. Whey-based media were in fact investigated for the production of value-added chemicals (e.g., succinic acid, lactic acid), antimicrobial peptides and probiotic biomasses [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Concentrated Buffalo Whey as Substrate for Probiotic Cultures and as Source of Bioactive Ingredients: A Local Circular Economy Approach towards Reuse of Wastewaters

et al. 2021

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Waste reduction and reuse is a crucial target of current research efforts. In this respect, the present study was focused on providing an example of local investment in a simple process configuration that converts whey into value-added compounds and allows recovery of a clean water stream. In particular, buffalo milk whey obtained during mozzarella manufacturing was ultrafiltered in-house on spiral membrane modules (20 kDa), and the two obtained fractions, namely the retentate and the permeate, provided by the dairy factory, were further processed during this work. The use of an additional nanofiltration step allowed the recovery of high-quality water to be reused in the production cycle (machine rinsing water within the facility) and/or in agriculture, also reducing disposal costs and the environmental impact. The ultrafiltration retentate, on the other hand, was spray-dried and the powder obtained was used as the main substrate for the cultivation of Lactobacillus fermentum, a widely studied probiotic with anti-inflammatory, immunomodulatory and cholesterol-lowering properties. In addition, the same sample was tested in vitro on a human keratinocytes model. Resuspended concentrated whey powder improved cell reparation rate in scratch assays, assisted through time-lapse video-microscopy. Overall these data support the potential of buffalo whey as a source of biologically active components and recyclable water in the frame of a local circular economy approach.

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Batch and fed-batch production of probiotic biomass and nisin in nutrient-supplemented whey media

Cited by 21 publications

References 21 publications

Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

Probiotics media: significance, challenges, and future perspective - a mini review

Concentrated Buffalo Whey as Substrate for Probiotic Cultures and as Source of Bioactive Ingredients: A Local Circular Economy Approach towards Reuse of Wastewaters

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