Batch cultivation ofMethylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions

Nn, Shah; Ml, Hanna; Rt, Taylor

doi:10.1002/(sici)1097-0290(19960120)49:2<161::aid-bit5>3.0.co;2-o

Cited by 60 publications

(39 citation statements)

References 28 publications

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“…The stored PHB in cells has been suggested to provide intracellular reducing equivalent to improve the TCE transformation capacity. The sMMO TCE degradation activity decreased to a negligible value following most of the PHB which was stored at a low level that disappeared without formate, while 30% to 40% of their TCE degradation activity was retained when the cells contained more PHB without formate after the same time [4]. In all cases, no free β-hydroxybutyrate was detected, indicating that the PHB lost upon storage was further metabolized by the cells.…”

Section: Epoxidation Activity Of Mmo (With Cu and Without Cu) And Intmentioning

confidence: 93%

“…Methanotrophic bacteria offer several advantages over other aerobic oxygenase-producing bacteria in degrading common groundwater contaminants, such as certain chlorinated aliphatic hydrocarbons, due to their high conversion rates and their utilization of methane as a nontoxic oxygenase inducer. They also have potential applications in the production of chemicals, such as epoxypropane, poly-β-hydroxybutyrate (poly-3-hydroxybutyrate; PHB) [1], methanol [2,3], and select higher alcohols, due to their nonspecific methane monooxygenase (MMO) enzyme systems [4].…”

Section: Introductionmentioning

confidence: 99%

“…The carbon dioxide produced from methane oxidation is partly emitted and partly incorporated into cell biomass via the serine pathway [5]. In certain methanotrophs, such as Methylosinus trichosporium OB3b and Methylococcus cupsulurus (Bath), a membranebound form of MMO (particulate methane monooxygenase [pMMO]) is produced when sufficient Cu is present, whereas a soluble form of MMO enzyme (soluble methane monooxygenase [sMMO]) is produced when Cu is inadequate [4,6]. The sMMO oxidizes a wide range of hydrocarbons (e.g., n-alkanes, n-alkenes, aromatic and alicyclic compounds, and many chlorinated solvents).…”

Section: Introductionmentioning

confidence: 99%

“…PHB, a naturally occurring biopolymer, is biodegradable and has tensile as well as thermal properties similar to those of synthetic plastic-like polypropylene [4]. PHB is accumulated as an intracellular carbon and energy storage material by a variety of microorganisms under nitrogen-, phosphate-, or oxygen-limiting condition.…”

Section: Introductionmentioning

confidence: 99%

“…The first step in the conversion of methane into PHB is carried out by nonspecific MMO enzyme systems. Resting cells of methanotrophs containing sMMO and pMMO both have a finite or intrinsic catalytic capacity for propene epoxidation due to a limiting supply of intracellular NADH reducing power [4,[18][19][20][21]. The limitation due to reducing equivalent availability can be offset by adding an external source of metabolic electron donors such as sodium formate or hydrogen gas.…”

Section: Introductionmentioning

confidence: 99%

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The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

Zhang

Xin

Chen

et al. 2008

Appl Biochem Biotechnol

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Methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the production of a biopolymer, poly-beta-hydroxybutyrate (poly-3-hydroxybutyrate; PHB). In contrast with methane monooxygenase (MMO) activity and ability of PHB synthesis of four kinds of methanotrophic bacteria Methylosinus trichosporium OB3b, M. trichosporium IMV3011, Methylococcus capsulatus HD6T, Methylomonas sp. GYJ3, and the mixture of the four kinds of strains, M. trichosporium OB3b is the highest of the four in the activity of propene epoxidation (10.72 nmol/min mg dry weight of cell [dwc]), the activity of naphthalene oxidation (22.7 mmol/mg dwc), and ability in synthesis of PHB(11% PHB content in per gram dry weight of cell in 84 h). It could be feasible to improve the MMO activity by mixing four kinds of methanotrophs. The MMO activity dramatically decreased when the cellular PHB accumulated in the second stage. The reason for this may be the dilution of the MMO system in the cells with increasing PHB contents. It has been found that the PHB contents at the level of 1-5% are beneficial to the cells for maintenance of MMO epoxidation activity when enough PHB have been accumulated. Moreover, it was also found that high particulate methane monooxygenase activity may contribute to the synthesis of PHB in the cell, which could be used to improve the yield of PHB in methanotrophs.

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Section: Epoxidation Activity Of Mmo (With Cu and Without Cu) And Intmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

Zhang

Xin

Chen

et al. 2008

Appl Biochem Biotechnol

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Attachment/detachment and trichloroethylene degradation-longevity of a resting cell Methylosinus trichosporium OB3b filter

Hanna

Taylor

2000

Biotechnol. Bioeng.

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We are investigating a methanotrophic filter strategy for the in situ bioremediation of low levels of chlorinated aliphatic, volatile organic chemicals (VOCs). It is based on the use of pregrown, resting cells, instead of growth‐nutrient stimulations. The economic feasibility of such a filter is dependent on its operational longevity at ground‐water temperatures. The latter, in turn, is dependent on several key parameters, such as the bacterial attachment densities reached during the injection of the microbial suspension and the subsequent detachment‐removal of cells from the filter over time. Scaled attachment/detachment experiments were carried out using a representative quartzitic sand in glass 1‐cm × 10‐cm columns to simulate a filter. A rosette‐dominated form of Methylosinus trichosporium OB3b was isolated and used in these and the subsequent catalytic longevity experiments. Its initial attachment, employing Higgins' medium phosphate buffer, pH 7.0 (HPB), was 7.0 to 8.0 × 108 bacteria/g of dry sand. This was elevated to ∼1.5 × 109 cells/g by including 1.0 mM MgCl2, 100 μM FeSO4, and 0.025% agar in the cell‐suspension loading buffer. These loading additives also increased the time required to reach 50% cell detachment with HPB alone from 5 days to ∼45 days. The functional longevity of a column biofilter, formed with resting‐state rosette‐enriched cells in the presence of the aforementioned additives, was determined at 21°C by challenging it with weekly 12 h, ∼250 ppb pulses of trichloroethylene (TCE). The column results indicate that for our attached‐cell filter to biodegrade TCE levels of several hundred ppb sufficiently, to <5 ppb, it will likely need replenishment at ∼8 week intervals, due to the instability of the endogenous whole‐cell soluble methane monooxygenase specific activity beyond that time period. This study represents the first time that anyone has shown that a rosette‐enriched substrain can be isolated from a well‐known methanotrophic strain and then stably cultured and utilized advantageously for a specific application—namely its improved attachment‐slowed detachment characteristics in a microbial filter.

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Methanol production from CO₂ by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011

Xin

Zhang

et al. 2007

J. Basic Microbiol.

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Methanol production from carbon dioxide was successfully achieved using resting cells of Methylosinus trichosporium IMV 3011 as biocatalysts. Carbon dioxide was reduced to methanol and extracellular methanol accumulation has been found in the carbon dioxide incubations. However, resting cells of methanotrophs have a finite or intrinsic methanol production capacity due to a limiting supply of intracellular reducing equivalent. It has been found that the catabolism of stored Poly-beta -Hydroxybutyrate (PHB) can provide intracellular reducing equivalents to improve the intrinsic methanol production capacity. The initial nitrogen and copper concentration in the culture medium were studied for the accumulation of PHB by M. trichosporium IMV 3011, to expand its potential uses in methanol production from carbon dioxide reduction. It appeared that the total methanol production capacity was increased with increasing PHB content in cells. Resting cells containing 38.6% PHB exhibited the highest total methanol production capacity. But higher PHB accumulation adversely affected the total methanol production capacity. The effects of methanol production process on the survival and recovery of M. trichosporium IMV 3011 were examined. The results showed that the methanol production from carbon dioxide reduction was not detrimental to the viability of methanotrophs.

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Batch cultivation ofMethylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions

Cited by 60 publications

References 28 publications

The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

Attachment/detachment and trichloroethylene degradation-longevity of a resting cell Methylosinus trichosporium OB3b filter

Methanol production from CO₂ by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011

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Batch cultivation ofMethylosinus trichosporium OB3b: V. Characterization of poly-β-hydroxybutyrate production under methane-dependent growth conditions

Cited by 60 publications

References 28 publications

The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs

Attachment/detachment and trichloroethylene degradation-longevity of a resting cell Methylosinus trichosporium OB3b filter

Methanol production from CO2 by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011

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Methanol production from CO₂ by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011