Batch fermentation of succinic acid from cheese whey by Actinobacillus succinogenes under variant medium composition

Omwene, Philip Isaac; Yağcıoğlu, Meltem; Öcal-Sarihan, Zehra Betül; Ertan, Fatma; Keris-Sen, Ulker D.; Keskinler, Bülent

doi:10.1007/s13205-021-02939-w

Cited by 10 publications

(11 citation statements)

References 40 publications

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“…Hence, the use of neutralizing agents is required to prevent the medium from becoming too acidic with the continuous formation of other organic acid byproducts, i.e. succinic, formic and acetic acids, during fermentation 41 . Furthermore, a study found that dissolved CO 2 was not sufficient enough to optimize organic acid formation when gaseous CO 2 was used as a sole inorganic carbon source donor in the fermentation of A. succinogenes ATCC 55,618 42 .…”

Section: Resultsmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Sci Rep

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The aim of this study was to establish an improved pretreatment and fermentation method i.e. immobilized cells for high recovery of fermentable sugars from palm kernel cake (PKC) and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of the fermentable sugar to lactic acid. The effects of oxalic acid concentrations (1–6% w/v) and residence times (1–5 h) on the sugar recovery were initially investigated and it was found that the highest mannose concentration was 25.1 g/L at the optimum hydrolysis conditions of 4 h and 3% (w/v) oxalic acid. The subsequent enzymatic saccharification of the pretreated PKC afforded the highest enzymatic digestibility with the recovered sugars amounting to 25.18 g/L and 9.14 g/L of mannose and glucose, respectively. Subsequently, the fermentability performance of PKC hydrolysate was evaluated and compared in terms of cultivation phases (i.e. mono and dual-phases), carbonate loadings (i.e. magnesium and sodium carbonates), and types of sugars (i.e. glucose and mannose). The highest titer of 19.4 g/L lactic acid was obtained from the fermentation involving A. succinogenes 130Z in dual-phase cultivation supplemented with 30 g/L of magnesium carbonate. Lactic acid production was further enhanced by using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes (A, B, C, and D) in the repeated batch cultivation of dual-phase fermentation producing 31.64 g/L of lactic acid. This work sheds light on the possibilities to enhance the utilization of PKC for lactic acid production via immobilized A. succinogenes 130Z.

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Section: Resultsmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Hence, the use of neutralizing agents is required to prevent the medium from becoming too acidic with the continuous formation of other organic acid byproducts, i.e. succinic, formic and acetic acids, during fermentation [42]. Furthermore, a study found that dissolved CO 2 was not su cient enough to optimize organic acid formation when gaseous CO 2 was used as a sole inorganic carbon source donor in the fermentation of A. succinogenes ATCC 55618 [43].…”

Section: Effects Of Carbonate Loading On Lactic Acid Productionmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from palm kernel cake by immobilized Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Preprint

View full text Add to dashboard Cite

The aim of this study was to establish an improved pretreatment and fermentation method i.e. immobilized cells for high recovery of fermentable sugars from PKC and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of lactic acid. The effects of oxalic acid concentrations (1–6% w/v) and residence times (1–5 h) on the sugar recovery were initially investigated and it was found that the highest mannose concentration was 25.1 g/L at the optimum hydrolysis conditions of 4 h and 3% (w/v) oxalic acid. The subsequent enzymatic saccharification of the pretreated PKC afforded the highest enzymatic digestibility with the recovered sugars amounting to 25.18 g/L and 9.14 g/L of mannose and glucose, respectively. Subsequently, the fermentability performance of PKC hydrolysate was evaluated and compared in terms of cultivation phases (i.e. mono and dual-phases), carbonate loadings (i.e. magnesium and sodium carbonates), and types of sugars (i.e. glucose and mannose). The highest titer of 19.4 g/L lactic acid was obtained from the fermentation involving A. succinogenes 130Z in dual-phase cultivation supplemented with 30 g/L of magnesium carbonate. Lactic acid production was further enhanced by using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes (A, B, C, and D) in the repeated batch cultivation of dual-phase fermentation producing 31.64 g/L of lactic acid. This work sheds light on the possibilities to enhance the utilization of PKC for lactic acid production via immobilized A. succinogenes 130Z.

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“…Among the bacterial strains, the Gram-negative bovine rumen-isolated A. succinogenes is deemed one of the best succinic acid producers due to its high acid concentration tolerance and high succinic acid yield from a variety of carbon sources generating ability [96]. Moreover, its attribute as a facultative anaerobic has contributed to reducing bio-succinic acid fermentation costs as aeration is not required [85].…”

Section: Succinic Acid Fermentation Biocatalystsmentioning

confidence: 99%

“…Sparging CO 2 as a pH buffer during fermentation is another method of maintaining the pH [96]. Besides being a driving force that regulates PEPCK activities in the succinic acid production pathway, the technique also aids in enhancing the gas-liquid mass transfer and reduces by-product formation [71].…”

Section: Phmentioning

confidence: 99%

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Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

et al. 2023

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Plastic pollution has placed a significant emphasis on the need for synthesising bioplastics, such as polybutylene succinate (PBS), which is derived from succinic acid. Furthermore, environmental concerns and the depletion of non-renewable fossil fuels have initiated an interest in exploring the biotechnological route of succinic acid production via fermentation. Consequently, oil palm biomass might be a prospective substitute for the costlier pure carbon source, which is more sustainable and cost-effective due to its abundance and high lignocellulosic content. The current review focuses on the potential of oil palm biomass utilisation to synthesise succinic acid and its associated bioplastics. The pretreatment and hydrolysis of various oil palm biomass and studies on bioplastics generation from oil palm biomass are also discussed. This review also identified the challenges of manufacturing succinic acid from oil palm biomass and included several recommendations.

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Batch fermentation of succinic acid from cheese whey by Actinobacillus succinogenes under variant medium composition

Cited by 10 publications

References 40 publications

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Biotechnological enhancement of lactic acid conversion from palm kernel cake by immobilized Actinobacillus succinogenes 130Z

Succinic Acid Production from Oil Palm Biomass: A Prospective Plastic Pollution Solution

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