In this study, a model of test beam in negative moment area is designed, and the slip characteristics of test beam under overlimit static load and variable amplitude cyclic load are studied, respectively. By constructing the relationship between shear stiffness and slip parameters of the test beam, the deformation calculation method of the test beam under different fatigue cycles is derived, and the accuracy of the calculation model is verified by experiments. The results show that the maximum slip value (0.038 mm) of static load after 10,000 times of limited fatigue load is increased by 58.3% compared with that before overlimit fatigue load is applied (0.024 mm). When the fatigue cycle is 0-2 million times, the total slip is between 0.019 and 0.026 mm and the residual slip percentage is between 4.17 and 8.33%. The maximum residual values are 0.022 mm, 0.027 mm, and 0.028 mm after 1.5 times, 2 times, and 3 times of overload and variable amplitude fatigue loads, respectively, and the slip values have no obvious fluctuation, all of which show good working performance. The average value of the ratio between the deformation value and the measured value of composite beams is 0.89, and the standard deviation is 4.86%. When the fatigue loading times are more than 2.8 million, the ratio between the calculated value and the measured value is less than 0.85, so the adaptability of the calculation model has certain limitations. On the whole, the calculation model proposed in this study fully considers the factors such as the stiffness and fatigue loading times of composite beams, and the error between the calculated results and the measured results is within 5%, with high accuracy, which can be used as a reference for the actual design.