The geoacoustic and physical properties of the bottom, as well as spatial distribution, are crucial factors in analyzing the underwater acoustic field structure and establishing a geoacoustic model. Acoustic inversion has been widely used as an economical and effective method to obtain multi-parameters of the bottom. Compared with traditional inversion methods based on acoustic propagation models, acoustic backscattering models are more suitable for multi-parameter inversion, because they contain more bottom information. In this study, a Bayesian inversion method based on an acoustic backscattering model is proposed to obtain bottom multi-parameters, including geoacoustic parameters (the sound speed and loss parameter), partial physical parameters of the sediment, and statistical parameters of the seafloor roughness and sediment heterogeneity. The bottom was viewed as a kind of fluid medium. A high-frequency backscattering model based on fluid theory was adopted as the forward model to fit the scattering strength between the model prediction and the measured data. The Bayesian inversion method was used to obtain the posterior probability density (PPD) of the inversion parameters. Parameter estimation, uncertainty, and correlation were acquired by calculating the maximum a posterior (MAP), the mean values, the one-dimensional marginal distributions of the PPD, and the covariance matrix. Finally, the high-frequency bottom backscattering strength from the Quinault Range site was employed for inversion tests. The estimated values and uncertainties of various bottom parameters are presented and compared with the directly measured bottom parameters. The comparison results demonstrate that the method proposed herein can be used to estimate the sediment/water sound speed ratio, the sediment/water density ratio, and the spectral exponent of the roughness spectrum effectively and reliably.