To meet the increasing raw material demand of the livestock and food sectors in our country is a need for new high-quality barley varieties that have a short vegetation period, resistance to lodging, homogeneous and plump grain, high grain and biomass yield, and compatible with the climate of the Central Anatolia Region. To meet this need, it was aimed to determine the appropriate lines by comparing 18 advanced barley lines belonging to the Transitional Zone Agricultural Research Institute with the standard varieties widely grown in the region. The trials were carried out in two locations, Eskişehir Center and Hamidiye fields of the Transitional Zone Agricultural Research Institute in the 2017-2018 crop year, in rainfed conditions. The trials were set up as a randomized complete block design with 4 replications in Eskişehir and 3 replications in Hamidiye. In the study, grain yield, days to heading, plant height, lodging score, thousand-grain weight, plump grain ratio, grain protein, and betaglucan ratio properties of the breeding material were investigated. According to the results of the analysis of variance by combining the location averages, the differences between genotypes in all parameters except the betaglucan ratio were found to be statistically significant. In the study were obtained that grain yield is between 4.56-6.67 t ha -1 ; days to heading are between 115.4 -124 days; plant height is between 81.6-101.4 cm; lodging score is between 0-100%; thousand-grain weight is between 35.7-47.4 g; the plump grain ratio is between 52.0-94.3%; grain protein content is between 10.05-12.75%; ß-glucan values are between 3.67-4.33% were obtained. Also, in the correlation analysis, a negative relationship was found significant between grain yield and days to heading, lodging score, and protein content characteristics, while a relationship was found significant to be positive with the plump grain ratio feature. When all the parameters are evaluated together, it is seen that lines 8, 11, 13, 14, 18, and 22 stand out as candidates for variety.