Bazi Bushen maintains intestinal homeostasis through inhibiting <scp>TLR4</scp>/<scp>NFκB</scp> signaling pathway and regulating gut microbiota in <scp>SAMP6</scp> mice

Xu, Zhe; Man, Shanshan; Gong, Bin; Li, Z.; Zhou, Huifang; Peng, Yanfei; Zhao, Shuwu; Hou, Yanli; Wang, Li; Bian, Yuhong

doi:10.1002/jsfa.12812

Cited by 7 publications

(2 citation statements)

References 60 publications

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“…Activation of TLR4 leads to NF-κB enters the nucleus from the cytoplasm, leading to the release of a large number of pro-inflammatory factors 31 . Upregulation of the TLR4/NF-κB inflammatory pathway in the mouse intestine can be observed in the SAMP6 mouse model, leading to an inflammatory response in the mouse intestine 32 . Mao et al .…”

Section: Discussionmentioning

confidence: 99%

Apple polysaccharide improves age-matched cognitive impairment and intestinal aging through microbiota-gut-brain axis

Zhang,

Zhong,

Wang

et al. 2024

Sci Rep

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The Apple polysaccharides (AP), extracted from the fruit of apple, has been used to treat multiple pathological diseases. In this study, we evaluated the effects of AP on cognitive impairment and intestinal aging in naturally aging mice. As a result, it was found that AP could improve spatial learning and memory impairment in aging mice through the Morris water maze experiment. Additionally, AP intervention can upregulate the expression of nerve growth factor (BDNF), postsynaptic marker (PSD95), and presynaptic marker (SYP) proteins. Moreover, AP can enhance total antioxidant capacity, reduce the level of pro-inflammatory cytokine, and inhibit the activation of the NF-κB signaling pathway, exerting anti-inflammatory and antioxidant functions. And the administration of AP restored intestinal mucosal barrier function, reduced the expression of aging and apoptosis related proteins. The administration of AP also altered the gut microbiota of mice. At the genus level, AP decreased the abundance of Helicobacter and Bilophila, while increased the abundance of Lactobacillus and Bacteroides. In summary, these data demonstrate that AP treatment can alleviate cognitive impairment, oxidative stress, and inflammatory reactions, repair the intestinal mucosal barrier, reduce intestinal aging, and alter specific microbial characteristics, ultimately improving the health of the elderly.

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Section: Discussionmentioning

confidence: 99%

Apple polysaccharide improves age-matched cognitive impairment and intestinal aging through microbiota-gut-brain axis

Zhang,

Zhong,

Wang

et al. 2024

Sci Rep

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“…The length of intestinal villi was calculated by Image Pro Plus 6.0 software. The protocol was performed as previously described ( Xu et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis

Zhang,

Li,

Chang

et al. 2024

Front. Microbiol.

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PurposeThe senescence-accelerated prone mouse 8 (SAMP8) is a widely used model for accelerating aging, especially in central aging. Mounting evidence indicates that the microbiota-gut-brain axis may be involved in the pathogenesis and progression of central aging-related diseases. This study aims to investigate whether Bazi Bushen capsule (BZBS) attenuates the deterioration of the intestinal function in the central aging animal model.MethodsIn our study, the SAMP8 mice were randomly divided into the model group, the BZ-low group (0.5 g/kg/d BZBS), the BZ-high group (1 g/kg/d BZBS) and the RAPA group (2 mg/kg/d rapamycin). Age-matched SAMR1 mice were used as the control group. Next, cognitive function was detected through Nissl staining and two-photon microscopy. The gut microbiota composition of fecal samples was analyzed by 16S rRNA gene sequencing. The Ileum tissue morphology was observed by hematoxylin and eosin staining, and the intestinal barrier function was observed by immunofluorescence. The expression of senescence-associated secretory phenotype (SASP) factors, including P53, TNF-α, NF-κB, IL-4, IL-6, and IL-10 was measured by real-time quantitative PCR. Macrophage infiltration and the proliferation and differentiation of intestinal cells were assessed by immunohistochemistry. We also detected the inflammasome and pyroptosis levels in ileum tissue by western blotting.ResultsBZBS improved the cognitive function and neuronal density of SAMP8 mice. BZBS also restored the intestinal villus structure and barrier function, which were damaged in SAMP8 mice. BZBS reduced the expression of SASP factors and the infiltration of macrophages in the ileum tissues, indicating a lower level of inflammation. BZBS enhanced the proliferation and differentiation of intestinal cells, which are essential for maintaining intestinal homeostasis. BZBS modulated the gut microbiota composition, by which BZBS inhibited the activation of inflammasomes and pyroptosis in the intestine.ConclusionBZBS could restore the dysbiosis of the gut microbiota and prevent the deterioration of intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis. These results suggested that BZBS attenuated the cognitive aging of SAMP8 mice, at least partially, by targeting the microbiota-gut-brain axis.

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Role of epigenetics in the regulation of skin aging and geroprotective intervention: A new sight

Pozzo,

Xu,

Lin

et al. 2024

Biomedicine & Pharmacotherapy

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Bazi Bushen maintains intestinal homeostasis through inhibiting TLR4/NFκB signaling pathway and regulating gut microbiota in SAMP6 mice

Cited by 7 publications

References 60 publications

Apple polysaccharide improves age-matched cognitive impairment and intestinal aging through microbiota-gut-brain axis

Apple polysaccharide improves age-matched cognitive impairment and intestinal aging through microbiota-gut-brain axis

Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis

Role of epigenetics in the regulation of skin aging and geroprotective intervention: A new sight

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