Bazı Gernik Buğdayların iPBS Markörleri Kullanılarak Moleküler Karakterizasyonu

Abstract

The genetic diversity among totally 21 wheat genotypes (16 Triticum dicoccum L. called emmer wheat and 5 Triticum durum L. called durum wheat) were investigated using 7 iPBS primers. iPBSs used in the current study generated 134 polymorphic from out of 136 bands for wheat genotypes. Characterization of the iPBS primers were performerd by calculation meanpolymorphism information content (PIC= 0.19), heterozygosity (H= 0.23), polymorphism ratio (P%= 97.92) and number of average polymorphic (19.14). Jaccard's diversity index varied between 0.0222 and 0.7843 with a mean 0.4677 for wheat genotypes. According to the sum of AMOVA, genetic diversity in emmer and durum wheats was significantly high with 77% variation within the population. On the other hand, genetic variation among populations was moderate with a value of 23%. Genetic diversity of populations were calculated by parameters including the mean number of alleles per locus (Na=1.269), effective allele number (Ne=1.31), Shannon information index (I=0.29), Nei's genetic diversity level (h=0.19), and Nei's unbiased genetic diversity level (uh=0.218). Results obtained from this study showed that iPBS molecular markers could be useful in determination of genetic relationships among wheat genotypes.