Language barriers and the communication difficulties of individuals with developmental disabilities are two major causes of communication problems that societies worldwide encounter. A particularly challenging group is hearing-impaired people who have difficulties with communication, reading, writing, learning, and social interactions, which have a substantial impact on their quality of life. This article focuses on detailing a Serbian Sign Language alphabet database and the method for creating it in order to provide a foundation for answering the various societal challenges of persons who use the Serbian language. In front of a computer camera, 41 people performed Serbian Sign Language sign movements that replicated the Serbian alphabet for this study’s aims. Hand and body key points were identified using the recorded video clips, and the numerical values of the identified key points were then stored in a database for further processing. In total, 8.346 video clips of people making recognized hand gestures were gathered, processed, classed, and archived. This paper provides a thorough technique that may be applied to comparable tasks and details the process of constructing a dataset based on Serbian Sign Language alphabet signs. This dataset was created using custom-made Python 3.11 software. Data regarding dynamic video clips that capture entire subject movement were incorporated into this dataset to fill in the gaps in other similar efforts based on static photographs. Thus, the purpose of this investigation is to employ innovative technology to support the community of hearing-impaired people in areas such as general inclusion, education, communication, and empowerment.