BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma

Mao, Chun-Guo; Jiang, Shasha; Wang, Xiaoyang; Tao, Shaolin; Ji, Bin; Mao, Constance; Yang, Yanlian; Hu, Zhiyuan; Tan, Lei; Jin, Hua; Tan, Qunyou; Huang, Yi; Deng, Bo

doi:10.7150/ijbs.61790

Cited by 13 publications

(6 citation statements)

References 54 publications

(62 reference statements)

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“…BCAR1 has been identified as a new promising biomarker for lung cancer [ 32 ]. Moreover, upregulated BCAR1 predicted poor prognosis in lung cancer patients and was associated with lymph node and distant metastasis and chemotherapy resistance in lung cancer [ 19 , 20 , 33 , 34 ]. In order to explore the specific mechanism of the proliferation and metastatic promotion of ASF1B in LUAD, we first carried out a correlation analysis of ASF1B and BCAR family genes, showing that ASF1B was positively correlated with most of the BCAR family genes at the mRNA level and especially with BCAR1.…”

Section: Discussionmentioning

confidence: 99%

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Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Jie

2021

Computational and Mathematical Methods in Medicine

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Background. ASF1B is a member of the histone H3–H4 chaperone antisilencing feature 1 (ASF1). ASF1B reportedly acts as an oncogene in several cancers including, breast cancer and cervical cancer. To date, the role of ASF1B in lung adenocarcinoma (LUAD) is not elucidated. Methods. The TCGA database, containing data for 33 cancer types, was used to explore the dysregulation and prognostic value of the ASF1B gene in pan-cancer data. R software packages and public databases/webservers were applied for bioinformatics and statistical analyses. Using in vitro models, immunoprecipitation and immunofluorescence were utilized to investigate if BCAR1 interacted with ASF1B in LUAD. Further, transfection experiments were performed to validate the expression pattern of ASF1B in LUAD and examine its regulating role in tumor-associated processes including tumor cell proliferation and migration. Results. ASF1B was found to be significantly elevated in LUAD and the majority of cancer types, except PCPG (pheochromocytoma and paraganglioma). The overexpression of ASF1B was associated with worse prognostic outcomes in most cancer types including LUAD. ASF1B was associated with lymph node metastasis, and in vitro, it promoted the proliferation and migration of LUAD cells. ASF1B knockdown suppressed LUAD cell proliferation and migration and also diminished the expression of cell cycle, metastasis, and EMT signaling-associated proteins. BCAR1 was found positively correlated and interacting with ASF1B, and BCAR1 overexpression reversed the effects of ASF1B knockdown in LUAD cells. Conclusion. These findings indicated that ASF1B plays a significant role in the tumor progression of LUAD and BCAR1 mediates the tumor-promotive effects of ASF1B, acting as an intermediate protein. Therefore, the ASF1B/BCAR1 axis might be regarded as a putative therapeutic target for LUAD.

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Section: Discussionmentioning

confidence: 99%

“…In addition, the knockdown of ASF1B was accompanied by the decreased expression of BCAR1. Interestingly, BCAR1 has been widely reported to regulate the cell cycle and EMT in cancer [ 16 , 20 , 34 ]. Our current data indicated that ASF1B might promote cell proliferation and migration of LUAD by regulating cell cycle and EMT both.…”

Section: Discussionmentioning

confidence: 99%

Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Jie

2021

Computational and Mathematical Methods in Medicine

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“…Additionally, 4, 6-diamidino-2-phenylindole (DAPI) was used to stain the cells’ nuclei ( 23 , 25 ). The primer sequences used by the probes are detailed in Supplementary Table S2 ( 26 ) ( Figure 1A ; Supplementary Figure S9 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of various hepatectomy procedures on circulating tumor cells in postoperative patients: a case-matched comparative study

Lei,

Wang,

Tian

et al. 2023

Front. Med.

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BackgroundThe objective of this study is to elucidate the prevalence of systemic circulating tumor cells (CTCs) prior to and following resection of hepatocellular carcinoma (HCC), and to compare the disparities in postoperative CTCs in terms of quantity and classifications between the open liver resection (OPEN) and laparoscopic liver resection (LAP) cohorts.Patients, materials, and methodsFrom September 2015 to May 2022, 32 consecutive HCC patients who underwent laparoscopic liver resection at Southwest Hospital were retrospectively enrolled in this study. The clinicopathological data were retrieved from a prospectively collected computer database. Patients in the OPEN group matched at a 1:1 ratio with patients who underwent open liver resection during the study period on age, gender, tumor size, number of tumors, tumor location, hepatitis B surface antigen (HBsAg) positivity, alpha-fetoprotein (AFP) level, TNM and Child-Pugh staging from the database of patients to form the control group. The Can-Patrol CTC enrichment technique was used to enrich and classify CTCS based on epithelial-mesenchymal transformation phenotypes. The endpoint was disease-free survival (DFS), and the Kaplan–Meier method and multiple Cox proportional risk model were used to analyze the influence of clinicopathological factors such as total CTCs and CTC phenotype on prognosis.ResultsThe mean age of the 64 patients with primary liver cancer was 52.92 years (23–71), and 89.1% were male. The postoperative CTC clearance rate was more significant in the OPEN group. The total residual CTC and phenotypic CTC of the LAP group were significantly higher than those of the OPEN group (p = 0.017, 0.012, 0.049, and 0.030, respectively), which may increase the possibility of metastasis (p = 0.042). In Kaplan–Meier analysis, DFS was associated with several clinicopathological risk factors, including Barcelona Clinical Liver Cancer (BCLC) stage, tumor size, and vascular invasion. Of these analyses, BCLC Stage [p = 0.043, HR (95% CI) =2.03(1.022–4.034)], AFP [p = 0.007, HR (95% CI) =1.947 (1.238–3.062)], the number of positive CTCs [p = 0.004, HR (95% CI) =9.607 (2.085–44.269)] and vascular invasion [p = 0.046, HR (95% CI) =0.475 (0.22–1.023)] were significantly associated with DFS.ConclusionIn comparison to conventional OPEN technology, LAP technology has the capacity to augment the quantity of epithelial, mixed, and mesenchymal circulating tumor cells (CTCs). Following the surgical procedure, there was a notable increase in the total CTCs, epithelial CTCs, and mixed CTCs within the LAP group, indicating a potential drawback of LAP in facilitating the release of CTCs.

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“…An increasing number of studies are being conducted on the progression of exosomes as novel mediators of intercellular communication in multiple types of cancers, including lung carcinogenesis and the tumor microenvironment (TME). Thus, exosomes are known for mediating intercellular communication during tumor development (11)(12)(13)(14). The current review provides an overview of the role of exosomes in NSCLC growth, metastasis, and immune response.…”

Section: Introductionmentioning

confidence: 99%

Role of exosomes in non-small cell lung cancer and EGFR-mutated lung cancer

Rao

Huang

et al. 2023

Front. Immunol.

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As an important mediator of information transfer between cells, exosomes play a unique role in regulating tumor growth, supporting vascular proliferation, tumor invasion, and metastasis. Exosomes are widely present in various body fluids, and therefore they can be used as a potential tool for non-invasive liquid biopsy. The present study reviews the role of exosomes in liquid biopsy, tumor microenvironment formation, and epithelial-mesenchymal transition in non-small cell lung cancer (NSCLC). By targeting epidermal growth factor receptor (EGFR) therapy as a first-line treatment for patients with NSCLC, this study also briefly describes the occurrence of EGRF+ exosomes and the role of exosomes and their contents in non-invasive detection and potential therapeutic targets in EGFR-mutated lung cancer.

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BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma

Cited by 13 publications

References 54 publications

Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

Effect of various hepatectomy procedures on circulating tumor cells in postoperative patients: a case-matched comparative study

Role of exosomes in non-small cell lung cancer and EGFR-mutated lung cancer

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