Bcl‐2 is a prognostic marker and its silencing inhibits recurrence in ameloblastomas

Abstract

Objectives Ameloblastomas are the most common odontogenic epithelial tumors with high recurrence rate. The aim of this study was to identify apoptosis‐related genes with recurrence of ameloblastomas and to evaluate its feasibility as a prognostic marker and as a target molecule preventing from recurrence. Materials and Methods Public microarray data were analyzed. To evaluate their expression in ameloblastoma patients, immunohistochemical staining was performed in 89 human ameloblastoma tissues. Quantitative PCR was performed by use of ameloblastoma cell line (AM‐1). Fluorescence activated cell sorting analysis and western blotting were conducted following transfection with siRNA. Further, AM‐1 cells were implanted in the renal subcapsular layer of immunodeficient mice. Results Microarray data analysis revealed that osteoprotegerin (OPG) and B‐cell lymphoma 2 (Bcl‐2) were the two most upregulated genes in ameloblastoma. Only Bcl‐2 expression was significantly (p = 0.020) associated with recurrence in conservative treatment group (n = 17) among 89 patients. Silencing of Bcl‐2 increased apoptosis in AM‐1 cells in vitro and inhibited tumor nodule formation of AM‐1 cells in vivo. Conclusion These results suggest that Bcl‐2 expression is a useful biomarker to predict recurrence of ameloblastomas, and as a therapeutic target molecule to prevent recurrence of ameloblastoma.

Identification of circ_0089153/miR‐608/EGFR p53 axis in ameloblastoma via MAPK signaling pathway

“…Ameloblastoma (AB), the most common odontogenic epithelial tumor, has locally aggressive propensity and a high rate of postoperative recurrence rate (Kim et al., 2019). Among all the categories of odontogenic epithelial tumors, AB has the highest incidence rate (approximately 30%–40%) in developing countries (Ahire et al., 2018; Fernandes et al., 2005), reaching 76.5% in the African population (Oginni et al., 2015).…”

Central granular cell odontogenic tumour associated with sepsis, osteonecrosis and osteomyelitis

“…Bcl‐2 expression in granular cells, found in our study, was reported by Meer et al and Mesquita et al, 8,11 while Bcl‐2 negativity was detected only by Chiang et al 4 Furthermore, Meer et al found Bcl‐2 positivity also in the odontogenic epithelium, these data suggest that there is variability in the expression of this protein. In keratocyst and ameloblastoma bcl‐2 expression seems to be associated with a higher rate of recurrence and aggressive behaviour, Bcl‐2 may play a role also in the expansion of this tumour 37,38 …”

MicroRNA-1-3p Suppresses Malignant Phenotypes of Ameloblastoma Through Down-Regulating Lysosomal Associated Membrane Protein 2-Mediated Autophagy

“…For each section, three fields were randomly selected (×200). The expression scores of Beclin1, LC3, and LAMP2 were on the grounds of staining intensity (no coloring: 0 point; light yellow: 1 point; brown yellow: 2 points; sepia: 3 points) and percentage of positive tumor cells (0–5%: 0 point; 6–25%: 1 point; 26–50%: 2 points; >50%: 3 points) ( 17 ). The final score was determined by staining intensity score × percentage of positive tumor cells (>4 scores: positive and 0–3 scores: negative).…”