BCL-G as a new candidate gene for immune responses in pigs: Bioinformatic analysis and functional characterization

Jiang, Pengfei; Li, Dengyun; Bi, Lijun; Zhang, Deli

doi:10.1016/j.vetimm.2012.08.001

Cited by 6 publications

(18 citation statements)

References 17 publications

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“…Furthermore, the cytokine responses in bronchoalveolar lavage fluid from SwIV-infected piglets are also identical to those observed for nasal lavage fluids of experimentally infected humans18. Recently, several studies have taken porcine genes such as BCL-G19, p58 IPK 20, and JAB121 as molecular models to study human diseases. These reports indicate that the pig can serve as an excellent animal model to study the pathogenesis of human influenza.…”

mentioning

confidence: 61%

Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection

Jiang

Zhou

Chen

et al. 2015

Sci Rep

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H1N1 swine influenza A virus (H1N1 SwIV) is one key subtype of influenza viruses with pandemic potential. MicroRNAs (miRNAs) are endogenous small RNA molecules that regulate gene expression. MiRNAs relevant with H1N1 SwIV have rarely been reported. To understand the biological functions of miRNAs during H1N1 SwIV infection, this study profiled differentially expressed (DE) miRNAs in pulmonary alveolar macrophages from piglets during the H1N1 SwIV infection using a deep sequencing approach, which was validated by quantitative real-time PCR. Compared to control group, 70 and 16 DE miRNAs were respectively identified on post-infection day (PID) 4 and PID 7. 56 DE miRNAs were identified between PID 4 and PID 7. Our results suggest that most host miRNAs are down-regulated to defend the H1N1 SwIV infection during the acute phase of swine influenza whereas their expression levels gradually return to normal during the recovery phase to avoid the occurrence of too severe porcine lung damage. In addition, targets of DE miRNAs were also obtained, for which bioinformatics analyses were performed. Our results would be useful for investigating the functions and regulatory mechanisms of miRNAs in human influenza because pig serves as an excellent animal model to study the pathogenesis of human influenza.

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mentioning

confidence: 61%

Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection

Jiang

Zhou

Chen

et al. 2015

Sci Rep

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“…see Jiang et al, 2012). However, the Bcl-G S protein isoform is specific to humans (Giam et al, 2012a).…”

Section: Homologymentioning

confidence: 99%

“…Differences are also apparent between Bcl-G S and Bcl-G L in their patterns of tissue expression (restricted to testes versus ubiquitous), subcellular distribution (cytosolic organelles versus diffuse) and interaction with other Bcl-2 family members (Bcl-G S only shows interaction) (Guo et al, 2001). Pig and mouse BCL2L14 encode only one BclG protein isoform (termed pBcl-G and mBcl-G, respectively), which contains BH2 and BH3 domains, similar to Bcl-G L (Giam et al, 2012a;Jiang et al, 2012). Phylogenetic analysis of Bcl-G protein sequences from 14 different species has revealed the presence of three different clades comprising human/apes, rodents and pigs/cattle, raising the possibility of species differences in Bcl-G action (Jiang et al, 2012).…”

Section: Identitymentioning

confidence: 99%

“…Pig and mouse BCL2L14 encode only one BclG protein isoform (termed pBcl-G and mBcl-G, respectively), which contains BH2 and BH3 domains, similar to Bcl-G L (Giam et al, 2012a;Jiang et al, 2012). Phylogenetic analysis of Bcl-G protein sequences from 14 different species has revealed the presence of three different clades comprising human/apes, rodents and pigs/cattle, raising the possibility of species differences in Bcl-G action (Jiang et al, 2012). The BCL2L14 promoter contains a cAMP responsive element, an interferon-γ-activated site (GAS) and an interferon regulatory factor element (IRF-E); interferon-α and γ co-operatively hyperactivate BCL2L14 expression in hepatoma cells (Zhang et al, 2006).…”

Section: Identitymentioning

confidence: 99%

“…Rather mBclG was capable of binding several novel partners, including transport particle protein (TRAPP) complex proteins, suggesting a potential role for mBclG in vesicle trafficking (Giam et al, 2012b). As regards pig Bcl-G, its ectopic expression in a porcine kidney cell line stimulates apoptosis induced by polyinosinic: polycytidylic acid (synthetic analogue of dsRNA and mimic of viral infection) (Jiang et al, 2012).…”

Section: Functionmentioning

confidence: 99%

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BCL2L14 (BCL2-like 14 (apoptosis facilitator))

Pickard¹,

Williams²

2014

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Deficiency of monoclonal non‐specific suppressor factor beta (MNSFB) promotes pregnancy loss in mice

Zhang

et al. 2015

Molecular Reproduction Devel

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Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of MNSFB using specific antibodies against it lead to failed embryo implantation in mice, the exact role of MNSFB at the fetal-maternal interface remains unclear. The present study generated conditional heterozygous Mnsfb-deficient (Mnsfb(+/) (-) ) mice using the LoxP/Cre system. Western-blot analyses showed that uterine MNSFB protein in Mnsfb(+/-) mice was remarkably down-regulated compared to that in the wild-type (Mnsfb(+/+) ) mice. The litter size of female Mnsfb(+/-) mice was significantly reduced, which corresponded to developmental failure of embryos beyond Day 11 of pregnancy. The expression level of MNSFB protein was also lower in the failing compared to the normal embryos. An aberrant interaction between the embryos of Day-4 pregnant wild-type mice and endometrial stromal cells of female Mnsfb(+/-) mice was observed in vitro. The uterine Day-5 abundance of P53, BAX, and BCL-G in pregnant Mnsfb(+/-) mice was significantly decreased compared to that of wild-type mice, whereas the expression of P27 and tumor necrosis factor alpha (TNFA) was elevated. By comparison, the levels of MNSFB and BAX proteins in human decidual tissues obtained from recurrent spontaneous miscarriage patients were significantly reduced compared to those obtained from legal medial abortion, highlighting the involvement of MNSFB in the pathogenesis of recurrent spontaneous miscarriage. Together, these results demonstrated that a deficiency in MNSFb is associated with pregnancy loss, probably through reduced P53 and/or increased TNFA production at the fetal-maternal interface.

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BCL-G as a new candidate gene for immune responses in pigs: Bioinformatic analysis and functional characterization

Cited by 6 publications

References 17 publications

Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection

Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection

BCL2L14 (BCL2-like 14 (apoptosis facilitator))

Deficiency of monoclonal non‐specific suppressor factor beta (MNSFB) promotes pregnancy loss in mice

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