Bcl11b transcription factor plays a role in the maintenance of the ameloblast-progenitors in mouse adult maxillary incisors

Katsuragi, Yoshihisa; Anraku, Junko; Nakatomi, Mitsushiro; Ida‐Yonemochi, Hiroko; Obata, Miki; Mishima, Yukio; Sakuraba, Yoshiyuki; Gondo, Yoichi; Kodama, Yasumitsu; Nishikawa, Atsushi; Takagi, Ritsuo; Ohshima, Hayato; Kominami, Ryo

doi:10.1016/j.mod.2013.05.002

Cited by 18 publications

(19 citation statements)

References 34 publications

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“…This finding is consistent with previous ChIP-on-chip analyses identifying Twi cis-regulatory modules in the vicinity of the CG9650 promoter (38, 53) during embryonic mesoderm expression. The mammalian orthologs of CG9650, Bcl11a and Bcl11b, are Kr-like transcription factors that have been associated with the maintenance of lymphoid and ameloblast progenitors, respectively (54,55). Bcl11b also is expressed in murine myogenic progenitors and disappears during differentiation, as is consistent with a possible conserved role in vertebrate muscle progenitor proliferation (56).…”

Section: Discussionmentioning

confidence: 89%

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Dequéant

Fagegaltier

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during RasV12 immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validated in vivo the transcription factor CG9650, the ortholog of mammalian Bcl11a/b, as a regulator of AMP proliferation predicted by our analysis. Our study demonstrates the power of time series synexpression analysis to characterize Drosophila embryonic progenitor lines and identify stem/progenitor cell regulators.

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Section: Discussionmentioning

confidence: 89%

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Dequéant

Fagegaltier

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…While the existence of Bcl11b expression in the early facial mesenchyme was previously known (Chan et al, 2007; Leid et al, 2004), the recent demonstrations of midfacial hypoplasia in Bcl11b −/− mice suggest a later role for this transcription factor in craniofacial development (Golonzhka et al, 2009b; Katsuragi et al, 2013). Indeed, we have found BCL11B expression to be intimately associated with suture formation and osteoblast differentiation during intramembranous craniofacial bone development.…”

Section: Discussionmentioning

confidence: 99%

“…Total and conditional mouse knockouts of Bcl11b have revealed its critical role in the development of thymocytes (Wakabayashi et al, 2003), neurons (Arlotta et al, 2005), skin (Golonzhka et al, 2009a) and teeth (Golonzhka et al, 2009b). While BCL11B expression in early facial mesenchyme has also previously been shown (Chan et al, 2007; Leid et al, 2004), an observation of midfacial hypoplasia in Bcl11b knockout mice has only recently been described (Golonzhka et al, 2009b; Katsuragi et al, 2013), and an understanding of how the known BCL11B expression domains could relate to this phenotype is lacking. Here, we show that BCL11B is widely expressed during suture development in murine embryos, and describe its expression patterns across a comprehensive range of craniofacial sutures that have not previously been shown to express BCL11B.…”

mentioning

confidence: 99%

BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development

Holmes

Bakel

Zhou

et al. 2015

Gene Expression Patterns

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Sutures, where neighboring craniofacial bones are separated by undifferentiated mesenchyme, are major growth sites during craniofacial development. Pathologic fusion of bones within sutures occurs in a wide variety of craniosynostosis conditions and can result in dysmorphic craniofacial growth and secondary neurologic deficits. Our knowledge of the genes involved in suture formation is poor. Here we describe the novel expression pattern of the BCL11B transcription factor protein during murine embryonic craniofacial bone formation. We examined BCL11B protein expression at E14.5, E16.5, and E18.5 in 14 major craniofacial sutures of C57BL/6J mice. We found BCL11B expression to be associated with all intramembranous craniofacial bones examined. The most striking aspects of BCL11B expression were its high levels in suture mesenchyme and increasingly complementary expression with RUNX2 in differentiating osteoblasts during development. BCL11B was also expressed in mesenchyme at the non-sutural edges of intramembranous bones. No expression was seen in osteoblasts involved in endochondral ossification of the cartilaginous cranial base. BCL11B is expressed to potentially regulate the transition of mesenchymal differentiation and suture formation within craniofacial intramembranous bone.

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“…Bcl11b encodes for a transcription factor with roles in embryonic tooth development (Katsuragi et al, 2013) and the development of T lymphocytes (Wakabayashi et al, 2003). It was not identified to be differentially expressed in any of the other tissues previously assayed in this ethanol model (Kaminen-Ahola et al, 2010a;Marjonen et al, 2015;Zhang et al, 2015), nor has it been identified in association with other models of prenatal ethanol exposure.…”

Section: Discussionmentioning

confidence: 87%

“…Many (Ptx3, Epha5, Bcl11b, Dcx, Phlda2, Cdx1, Zscan10, and Ddr2) had a known association with a growth phenotype, though often this was reported postnatally rather than prenatally (Chawengsaksophak et al, 1997;Katsuragi et al, 2013;Kraus et al, 2014;Labrador et al, 2001;Mamiya et al, 2008;Tunster et al, 2014;Werner et al, 2012;Witasp et al, 2014). The presence of genes with known roles in growth in our list provided assurance that the filtering conducted was valid, and that the resultant gene list was likely to be involved in the growth phenotype.…”

Section: Differentially Expressed Genes In Ethanol-exposed Male Embryosmentioning

confidence: 99%

A study of the consequences of early gestational ethanol and/or nicotine exposure in the mouse

Yamada¹

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The in utero environment is a critical determinant of the immediate and future health of the developing fetus. In Australia, two of the most commonly used drugs during pregnancy are alcohol and nicotine. Prolonged periods of gestational exposure to alcohol and nicotine, separately, have been associated with a range of adverse outcomes in the offspring, in both humans and experimental animal models. The consequences of ethanol and nicotine exposure restricted to early gestation on resultant offspring are poorly characterised, and few animal models exist to build greater understanding. Moreover, the immediate molecular changes associated with exposures to ethanol and/or nicotine restricted to early gestation has yet to be described.This thesis utilised mouse models of early gestational ethanol and/or nicotine exposure to explore the impact of each of these exposures on offspring growth, as well as their associated transcriptional and epigenetic changes. In each model, pregnant C57BL/6 dams were provided with ad libitum access to solutions of 10% ethanol and/or 100 µg/ml nicotine from fertilisation (0.5 dpc) to midgestation (8.5 dpc). The exposure window utilised in each of the models is developmentally equivalent to the first three to four weeks of a human pregnancy.Previously, our laboratory identified reduced growth, microcephaly, and gene expression changes in the liver and hippocampus of the postnatal offspring of the model of prenatal ethanol exposure utilised in this thesis. In contrast, at mid-gestation (9.5 dpc), there was no evidence of developmental delay (somite number), reduced growth (crown-rump length), or microcephaly.Further, a genome-wide gene expression microarray (targeting 30 855 genes) and a screen of the expression of 641 microRNAs, identified only one gene (Bcl11b) as differentially expressed in male 9.5 dpc whole embryos following the ethanol exposure. These data suggest that there is a delay between the ethanol exposure and the onset of adverse phenotypes -arguing that the embryo is somehow able to form a memory of the ethanol exposure. The absence of widespread transcriptional changes suggests that changes in embryonic mRNA and microRNA expression are unlikely to be major contributors to the memory of the exposure.A early gestational model of nicotine exposure was newly established in this thesis. This nicotine exposure resulted in an average serum cotinine (metabolite of nicotine) concentration similar to that reported in humans who smoke 10 to 15 cigarettes per day. This exposure was sufficient to induce a reduction in body size both at mid-gestation and postnatally, but not at late gestation. Furthermore, the increased expression of Zscan10 in the whole male 9.5 dpc embryo was identified and validated across multiple cohorts. Zscan10 encodes for a transcription factor expressed throughout development, with a proposed role in the regulation of progenitor cells. The increased expression of Zscan10 in the nicotine-exposed embryos was associated with a reduction in DNA methylation ...

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Bcl11b transcription factor plays a role in the maintenance of the ameloblast-progenitors in mouse adult maxillary incisors

Cited by 18 publications

References 34 publications

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development

A study of the consequences of early gestational ethanol and/or nicotine exposure in the mouse

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