Bcr-Abl is a “Molecular Switch” for the Decision for Growth and Differentiation in Hematopoietic Stem Cells

Era, Takumi

doi:10.1007/bf02982716

Cited by 11 publications

(5 citation statements)

References 93 publications

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“…This growth may be related to an increased level of mRNA for the IL-3R͞common ␤ chain in the CD34 Ϫ cells. The shift from SCF-dependent growth to a requirement for other cytokines such as IL-3 is a plausible early differentiation event, permitting the body to separately regulate the proliferation of different stages of precursor cells (35)(36)(37). The present studies show that this shift is related to a failure of SCF to cause detectable c-Kit tyrosine phosphorylation in the CD34 Ϫ cells.…”

Section: Resultsmentioning

confidence: 50%

Two types of precursor cells in a multipotential hematopoietic cell line

Kluger²,

Zheng³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The biochemistry of early stages of hematopoietic differentiation is difficult to study because only relatively small numbers of precursor cells are available. The murine EML cell line is a multipotential cell line that can be used to model some of these steps. We found that the lineage ؊ EML precursor cells can be separated into two populations based on cell surface markers including CD34. Both populations contain similar levels of stem cell factor (SCF) receptor (c-Kit) but only the CD34 ؉ population shows a growth response when treated with SCF. Conversely, the CD34 ؊ population will grow in the presence of the cytokine IL-3. The human ␤-globin locus control region hypersensitive site 2 plays different roles on ␤-globin transcription in the CD34 ؉ and CD34 ؊ populations. The two populations are present in about equal amounts in culture, and the CD34 ؉ population rapidly regenerates the mixed population when grown in the presence of SCF. We suggest that this system may mimic a normal developmental transition in hematopoiesis.c-Kit ͉ CD34 ͉ EML cells ͉ hypersensitive site 2

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Section: Resultsmentioning

confidence: 50%

Two types of precursor cells in a multipotential hematopoietic cell line

Kluger²,

Zheng³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…In vitro studies done by Era [11] and Buet et al . [12] showed two novel aspects of the differentiation of hematopoietic stem cells.…”

Section: Discussionmentioning

confidence: 99%

Improvement of platelet dysfunction in chronic myelogenous leukemia following treatment with imatinib: a case report

Shimabukuro‐Vornhagen

Rothe

Nogová

et al. 2011

J Med Case Reports

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IntroductionIn patients with chronic myeloid leukemia, tyrosine kinase inhibitors suppress the BCR-ABL+ clone and often induce complete molecular remissions. Megakaryocytes in such patients have been shown to be derived from the BCR-ABL+ clone, and abnormal platelet function is frequent in chronic myeloid leukemia. However, little is known about the influence of modern targeted therapy on chronic myeloid leukemia-associated platelet disorders.Case presentationWe report the case of a massive hemorrhage in a 32-year-old Caucasian man caused by chronic myeloid leukemia-associated platelet dysfunction, which improved after treatment with imatinib.ConclusionThis report demonstrates that platelet dysfunction and bleeding disorder in BCR-ABL+ chronic myeloid leukemia can successfully be treated with imatinib. We suggest the monitoring of platelet function in future studies using imatinib to treat patients with chronic myeloid leukemia.

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“…Recently, the tetracycline‐based conditional gene expression method has been used successfully with the OP9 system (Era & Witte 2000; Era 2002; Kitajima et al . 2002).…”

Section: Introductionmentioning

confidence: 99%

Differentiation status dependent function of FOG‐1

Tanaka

Zheng²,

Kitajima³

et al. 2004

Genes to Cells

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The molecular interactions between transcription factors and cofactors play crucial roles in various biological processes, including haematopoiesis. FOG-1 is a cognate cofactor of GATA-1, and the FOG-1/GATA-1 complex is essential for the haematopoietic differentiation of erythroid cells and megakaryocytes. In order to elucidate the biological functions of FOG-1 in the different contexts of cell differentiation, we analysed the effects of FOG-1 expression on haematopoietic cell differentiation, using a combination of in vitro differentiation of mouse embryonic stem (ES) cells and conditional gene expression. FOG-1 suppressed the proliferation of primitive and definitive erythroid cells in all stages of differentiation. However, FOG-1 inhibited and enhanced megakaryopoiesis in the early and late differentiation stages, respectively, through different molecular mechanisms. In addition, FOG-1 inhibited the proliferation of ES cells, the molecular mechanism of which differs from those of erythroid and megakaryocytic cells. These results suggest that FOG-1 functions in a cell differentiation context-dependent manner.

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Bcr-Abl is a “Molecular Switch” for the Decision for Growth and Differentiation in Hematopoietic Stem Cells

Cited by 11 publications

References 93 publications

Two types of precursor cells in a multipotential hematopoietic cell line

Two types of precursor cells in a multipotential hematopoietic cell line

Improvement of platelet dysfunction in chronic myelogenous leukemia following treatment with imatinib: a case report

Differentiation status dependent function of FOG‐1

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